A5078745988- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- HIV/AIDS drug development and treatment
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
- Long-Term Effects of COVID-19
- Acute Lymphoblastic Leukemia research
- COVID-19 and Mental Health
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Biochemical and Molecular Research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Misinformation and Its Impacts
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Vaccine Coverage and Hesitancy
- COVID-19 epidemiological studies
- T-cell and B-cell Immunology
- Sex and Gender in Healthcare
- Salivary Gland Disorders and Functions
Ludwig-Maximilians-Universität München
2020-2024
German Center for Infection Research
2021-2024
LMU Klinikum
2022
Institute of Medical Microbiology and Hygiene
2021
Charité - Universitätsmedizin Berlin
2014-2017
Disease manifestations in COVID-19 range from mild to severe illness associated with a dysregulated innate immune response. Alterations function and regeneration of dendritic cells (DCs) monocytes may contribute immunopathology influence adaptive responses patients. We analyzed circulating DC monocyte subsets 65 hospitalized patients mild/moderate or disease acute recovery healthy controls. Persisting reduction all subpopulations was accompanied by an expansion proliferating Lineage-HLADR+...
The importance of pre-existing immune responses to seasonal endemic coronaviruses (HCoVs) for the susceptibility SARS-CoV-2 infection and course COVID-19 is subject an ongoing scientific debate. Recent studies postulate that previous HCoV infections can either have a slightly protective or no effect on pathogenesis and, consequently, be neglected risk stratification. Challenging this notion, we provide evidence pre-existing, anti-nucleocapsid antibodies against α-coronaviruses S2...
On November 26, 2021, the World Health Organization classified B.1.1.529 as a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant of concern (VoC), named omicron. Spike-gene dropouts in conventional SARS-CoV-2 PCR systems have been reported over last weeks indirect diagnostic evidence for identification Here, we report combination PCRs specific heavily mutated sites spike gene and nanopore-based full-length genome sequencing rapid sensitive first four COVID-19 patients...
Resting CD4 T cells resist productive HIV-1 infection. The HIV-2/simian immunodeficiency virus protein viral accessory X (Vpx) renders these permissive to infection, presumably by alleviating blocks at cytoplasmic reverse transcription and subsequent nuclear import of reverse-transcription/pre-integration complexes (RTC/PICs). Here, spatial analyses using quantitative imaging techniques reveal that capsids containing RTC/PICs are readily imported into the nucleus, recruit host dependency...
High infection rates among healthcare personnel in an uncontained pandemic can paralyze health systems due to staff shortages. Risk constellations and of seroconversion for workers (HCWs) during the first wave severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are still largely unclear.Healthcare (n = 300) on different organizational units LMU Munich University Hospital were included followed this prospective longitudinal study from 24 March until 7 July 2020. Participants...
Abstract Individuals with hematologic malignancies are at increased risk for severe coronavirus disease 2019 (COVID-19), yet profound analyses of COVID-19 vaccine-induced immunity scarce. Here we present an observational study expanded methodological analysis a longitudinal, primarily BNT162b2 mRNA-vaccinated cohort 60 infection-naive individuals B cell lymphomas and multiple myeloma. We show that many these individuals, despite markedly lower anti-spike IgG titers, rapidly develop potent...
Siglec-1 (sialoadhesin, CD169) is a surface receptor on human cells that mediates trans-enhancement of HIV-1 infection through recognition sialic acid moieties in virus membrane gangliosides. Here, we demonstrate mouse Siglec-1, expressed the primary macrophages an interferon-α-responsive manner, captures murine leukemia (MLV) particles and their transfer to proliferating lymphocytes. The MLV B-cells was markedly more efficient than T-cells. major structural protein particles, Gag,...
Abstract Beim metabolischen Glykoengineering (MGE) werden Zellen und Tiere mit nichtnatürlichen Derivaten von Monosacchariden behandelt. Diese nach ihrer Aufnahme ins Zytosol metabolisiert anschließend auf neusynthetisierten Glykokonjugaten exprimiert. MGE wurde zuerst für sialylierte Glykane realisiert, N‐Acyl‐modifizierten Mannosaminen als Vorstufen nichtnatürliche Sialinsäuren. Voraussetzung ist die Promiskuität der Enzyme des Roseman‐Warren‐Biosyntheseweges. tolerieren spezifische...
Although some COVID-19 patients maintain SARS-CoV-2-specific serum immunoglobulin G (IgG) for more than 6 months postinfection, others eventually lose IgG levels. We assessed the persistence of B cells in 17 patients, 5 whom had lost specific IgGs after 5-8 months. Differentiation blood-derived vitro revealed persistent all whereas IgA were maintained 11. Antibodies derived from cultured blocked binding viral receptor-binding domain (RBD) to cellular receptor ACE-2, neutralizing activity...
The mucosal immunity is crucial for restricting SARS-CoV-2 at its entry site. Intramuscularly applied vaccines against stimulate high levels of neutralizing Abs in serum, but the impact these intramuscular vaccinations on features less clear. Here, we analyzed kinetic and functional properties anti-SARS-CoV-2 saliva after vaccination with BNT162b2. We a total 24 healthy donors longitudinally up to 16 months. found that specific IgG appeared second vaccination, declined thereafter reappeared...
Abstract Purpose To determine risk factors for coronavirus disease 2019 (COVID-19) in healthcare workers (HCWs), characterize symptoms, and evaluate preventive measures against SARS-CoV-2 spread hospitals. Methods In a cross-sectional study conducted between May 27 August 12, 2020, after the first wave of COVID-19 pandemic, we obtained serological, epidemiological, occupational as well COVID-19-related data at quaternary care, multicenter hospital Munich, Germany. Results 7554 HCWs...
Abstract The emergence of more transmissible or aggressive variants SARS‐CoV‐2 requires the development antiviral medication that is quickly adjustable to evolving viral escape mutations. Here we report synthesis chemically stabilized small interfering RNA (siRNA) against SARS‐CoV‐2. siRNA can be further modified with receptor ligands such as peptides using Cu I ‐catalysed click‐chemistry. We demonstrate optimized siRNAs reduce loads and virus‐induced cytotoxicity by up five orders magnitude...
Due to its position at the outermost of glycans, sialic acid is involved in a myriad physiological and pathophysiological cell functions such as host-pathogen interactions, immune regulation, tumor evasion. Inhibitors surface sialylation could be useful tool cancer, immune, antibiotic, or antiviral therapy. In this work, four different C-3 modified N-acetylmannosamine analogs were tested potential inhibitors sialylation. Peracetylated 2-acetylamino-2-deoxy-3-O-methyl-D-mannose decreases...
Abstract Disease manifestations in COVID-19 range from mild to severe illness associated with a dysregulated innate immune response. Alterations function and regeneration of dendritic cells (DC) monocytes may contribute immunopathology influence adaptive responses patients. We analyzed circulating DC monocyte subsets 65 hospitalized patients mild/moderate or disease acute recovery healthy controls. Persisting reduction all subpopulations was accompanied by an expansion proliferating Lineage...
Abstract In resource-limited or point-of-care settings, rapid diagnostic tests (RDTs), that aim to simultaneously detect HIV antibodies and p24 capsid (p24CA) antigen with high sensitivity, can pose important alternatives screen for early infections. We evaluated the performance of antibody components old novel version Determine™ HIV-1/2 Ag/Ab Combo RDTs in parallel quantifications a fourth-generation antigen/antibody immunoassay (4G-EIA), p24CA (p24CA-EIA), immunoblots, nucleic acid...
Synthetically accessible C6-analogs of<italic>N</italic>-acetylmannosamine (ManNAc) were tested as potential inhibitors of the bifunctional UDP-<italic>N</italic>-acetylglucosamine-2-epimerase/<italic>N</italic>-acetylmannosamine kinase (GNE/MNK), key enzyme sialic acid biosynthesis.
SAMHD1 mediates resistance to anti-cancer nucleoside analogues, including cytarabine, decitabine, and nelarabine that are commonly used for the treatment of leukaemia, through cleavage their triphosphorylated forms. Hence, inhibitors promising candidates sensitisation leukaemia cells analogue-based therapy. Here, we investigated effects cytosine analogue CNDAC, which has been proposed be a inhibitor, in context SAMHD1.
Abstract We aimed to develop a simple predictive model that enables health care workers (HCWs) self-assess pandemic-related psychological distress in order assist them find support avert adverse distress-related outcomes. In pilot study, we recruited and followed longitudinally 220 HCWs at the Hospital of Ludwig Maximilian University Munich (H-LMU) during first wave COVID-19 pandemic (March–July 2020). this sample, evaluated whether machine-learning with sociodemographic, epidemiological,...
Abstract Purpose To explore occupational and non-occupational risk protective factors for the coronavirus disease 2019 (COVID-19) in healthcare workers (HCWs). Methods Serum specimens questionnaire data were obtained between October 7 December 16, 2021 from COVID-19-vaccinated HCWs at a quaternary care hospital Munich, Germany, analyzed RisCoin Study. Results Of 3,696 participants evaluated, 6.6% have had COVID-19 least once. Multivariate logistic regression analysis identified working...
Immune cell phenotyping frequently detects lineage-unrelated receptors. Here, we report that surface receptors can be transferred from primary macrophages to CD4 T cells and identify the Fcγ receptor CD32 as driver cargo of this trogocytotic transfer. Filamentous CD32+ nanoprotrusions deposit distinct plasma membrane patches onto target cells. Transferred confer migration adhesion properties, macrophage-derived render resting susceptible infection by serving hotspots for HIV-1 binding....
Abstract Neutralizing antibodies (NAbs), and their concentration in sera of convalescents vaccinees are a correlate protection from COVID-19. The antibody concentrations clinical samples that neutralize SARS-CoV-2 difficult very cumbersome to assess with conventional virus neutralization tests (cVNTs), which require work the infectious biosafety level 3 containment precautions. Alternative (VNTs) currently use mostly surrogate based on direct or competitive enzyme immunoassays viral vectors...