A5002588358- Sphingolipid Metabolism and Signaling
- Pain Mechanisms and Treatments
- Phagocytosis and Immune Regulation
- Cannabis and Cannabinoid Research
- Analytical Methods in Pharmaceuticals
- Inflammatory mediators and NSAID effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Autophagy in Disease and Therapy
- Lipid Membrane Structure and Behavior
- Pesticide Residue Analysis and Safety
- HIV/AIDS drug development and treatment
- Pharmacological Effects of Natural Compounds
- Eicosanoids and Hypertension Pharmacology
- Immune Response and Inflammation
- Endoplasmic Reticulum Stress and Disease
- Neuropeptides and Animal Physiology
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Chromatography
- Asthma and respiratory diseases
- Acute Myeloid Leukemia Research
- Fatty Acid Research and Health
Goethe University Frankfurt
2016-2025
University Hospital Frankfurt
2013-2025
RELX Group (United States)
2017
Endokrinologikum
2016
Goethe Institute
2014
Witten/Herdecke University
2014
University Medical Center Freiburg
2007-2010
Instituto de Tecnología Química
2006-2008
University of the Basque Country
2006-2007
Abstract Herbal mixtures like ‘Spice’ with potentially bioactive ingredients were available in many European countries since 2004 and are still widely used as a substitute for cannabis, although merchandized ‘herbal incense’. After gaining high degree of popularity 2008, big quantities these drugs sold. In December synthetic cannabinoids identified the which not declared ingredients: C 8 homolog non‐classical cannabinoid CP‐47,497 (CP‐47,497‐C8) cannabimimetic aminoalkylindole called...
Genetic and epigenetic mechanisms play important roles in protein expression, although at different levels. variations can alter CpG sites thus influence the regulation of mRNA providing an increasingly recognized mechanism functional consequences genetic polymorphisms. One those effects is association reduced μ-opioid receptor expression with variant N40D (OPRM1 118A>G, rs1799971) that causes amino acid exchange extracellular terminal receptor. We report nucleotide gene position +118...
Vasodilatory prostaglandins play a key role in neurovascular coupling (NVC), the tight link between neuronal activity and local cerebral blood flow, but their precise identity, cellular origin receptors involved remain unclear. Here we show rats that NMDA-induced vasodilation hemodynamic responses evoked by whisker stimulation involve cyclooxygenase-2 (COX-2) activation of prostaglandin E2 (PgE2) EP2 EP4. Using liquid chromatography-electrospray ionization-tandem mass spectrometry,...
Abstract Preclinical studies have demonstrated that the endocannabinoid system (ECS) plays an important role in protection against intestinal inflammation and colorectal cancer (CRC); however, human data are scarce. We determined members of ECS related components ‘endocannabinoidome’ patients with inflammatory bowel disease (IBD) CRC, compared them to control subjects. Anandamide (AEA) oleoylethanolamide (OEA) were increased plasma ulcerative colitis (UC) Crohn’s (CD) while...
Specific somatosensations may be processed by different subsets of primary afferents. C-fibers expressing heat-sensitive TRPV1 channels are proposed, for example, to heat but not mechanical pain detectors. To phenotype in rats the sensory function + afferents, we rapidly and selectively silenced only their activity, introducing membrane-impermeant sodium channel blocker QX-314 into these axons via pore. Using tandem mass spectrometry show that upon activation with capsaicin, accumulates...
// Georgios Grammatikos 1, 2 , Niklas Schoell Nerea Ferreirós 3 Dimitra Bon 4 Eva Herrmann Harald Farnik Verena Köberle Albrecht Piiper Stefan Zeuzem Bernd Kronenberger Oliver Waidmann 2, * Josef Pfeilschifter 1 Goethe University Hospital, Pharmazentrum Frankfurt, Institut für Allgemeine Pharmakologie und Toxikologie, Frankfurt am Main, Germany Medizinische Klinik klinische Pharmakologie, University, Department of Medicine, Institute Biostatistics and Mathematical Modelling,...
Resolution of acute inflammation is an active process coordinated by proresolving lipid mediators (SPMs) such as lipoxins (LXs) and resolvins (Rvs), which are formed the concerted action 2 lipoxygenases (LOs). Because exact molecular mechanisms SPM biosynthesis not completely understood, we aimed to investigate LX D-type Rv formation in human leukocytes HEK293T cells overexpressing leukotriene (LT) pathway enzymes. Activity assays precursor (15-hydroxyeicosatetraenoic acids,...
Based on increasing evidence suggesting that MS pathology involves alterations in bioactive lipid metabolism, the present analysis was aimed at generating a complex serum lipid-biomarker. Using unsupervised machine-learning, implemented as emergent self-organizing maps of neuronal networks, swarm intelligence and Minimum Curvilinear Embedding, cluster structure found input data space comprising concentrations d = 43 different lipid-markers various classes. The coincided largely with clinical...
Background Autotaxin (ATX) and its product lysophosphatidic acid (LPA) are considered to be involved in the development of liver fibrosis elevated levels serum ATX have been found patients with hepatitis C virus associated fibrosis. However, clinical role systemic stages cirrhosis was unknown. Here we investigated relation severity as well prognosis cirrhotic patients. Methods Patients were prospectively enrolled followed until death, transplantation or last contact. Blood samples drawn at...
Bioactive lipids contribute to the pathophysiology of multiple sclerosis. Here, we show that lysophosphatidic acids (LPAs) are dysregulated in sclerosis (MS) and functionally relevant this disease. LPAs autotaxin, major enzyme producing extracellular LPAs, were analyzed serum cerebrospinal fluid a cross-sectional population MS patients compared with respective data from mice experimental autoimmune encephalomyelitis (EAE) model, spontaneous EAE TCR1640 mice, Lpar2 -/- mice. Serum reduced...
Endocannabinoids may modify cancer development, progression and associated pain. We determined whether cancer-evoked dysregulations in this system become manifest altered tissue plasma endocannabinoids.Endocannabinoid changes due to were explored a local metastatic syngeneic mouse melanoma model. Endocannabinoid stratification human was cross-sectionally assessed the of 304 patients (147 men, 157 women, aged 32 - 87 years) suffering from several types at Roman Numeral Staging between I IVc,...