A5079132237- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Mycobacterium research and diagnosis
- Viral Infections and Immunology Research
- COVID-19 Clinical Research Studies
- Parvovirus B19 Infection Studies
- Hepatitis Viruses Studies and Epidemiology
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Pneumonia and Respiratory Infections
- Metallurgy and Material Forming
- Viral-associated cancers and disorders
- Mechanical Engineering and Vibrations Research
- Polyomavirus and related diseases
- Long-Term Effects of COVID-19
- Infectious Encephalopathies and Encephalitis
- Plant Virus Research Studies
- Poxvirus research and outbreaks
- COVID-19 and healthcare impacts
- Gear and Bearing Dynamics Analysis
- Hematopoietic Stem Cell Transplantation
Institute of Medical Microbiology and Hygiene
2019-2024
German Center for Infection Research
2015-2024
Institute of Virology of the Slovak Academy of Sciences
2015-2023
University of Tübingen
2019-2022
Universität Ulm
2021
University Medical Center Freiburg
2021
German Cancer Research Center
2021
University Children's Hospital Tübingen
2019-2021
Medizinische Hochschule Hannover
2011-2021
Heidelberg University
2021
Fast and reliable detection of patients with severe heterogeneous illnesses is a major goal precision medicine1,2. Patients leukaemia can be identified using machine learning on the basis their blood transcriptomes3. However, there an increasing divide between what technically possible allowed, because privacy legislation4,5. Here, to facilitate integration any medical data from owner worldwide without violating laws, we introduce Swarm Learning-a decentralized machine-learning approach that...
<h3>Importance</h3> School and daycare closures were enforced as measures to confine the novel coronavirus disease 2019 (COVID-19) pandemic, based on assumption that young children may play a key role in severe acute respiratory 2 (SARS-CoV-2) spread. Given grave consequences of contact restrictions for children, better understanding their contribution COVID-19 pandemic is great importance. <h3>Objective</h3> To describe rate SARS-CoV-2 infections seroprevalence antibodies aged 1 10 years,...
Abstract Currently, 88 different Human Adenovirus (HAdV) types are grouped into seven HAdV species A to G. Most (57) belong HAdV-D. Recombination between capsid genes (hexon, penton and fiber) is the main factor contributing diversity in Noteworthy, HAdV-C contains so far only five types, although highly prevalent clinically significant immunosuppressed patients. Therefore, evolution of was studied by generating 51 complete genome sequences from circulating strains. Clustering whole...
Advances in next-generation sequencing (NGS) technologies allow comprehensive studies of genetic diversity over the entire genome human cytomegalovirus (HCMV), a significant pathogen for immunocompromised individuals.Next-generation was performed on target enriched sequence libraries prepared directly from variety clinical specimens (blood, urine, breast milk, respiratory samples, biopsies, and vitreous humor) obtained longitudinally or different anatomical compartments 20 HCMV-infected...
Abstract The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total 207 datasets generated in this previous studies using target enrichment high-throughput sequencing analyzed, the process enabling determination genome sequences for 91 strains. Key findings that (i) it is important to monitor quality libraries investigating...
Human adenovirus (HAdV) infection after hematopoietic stem cell transplantation (HSCT) is associated with significant morbidity and mortality in children. The optimal surveillance treatment strategies are under discussion. Here, we present data from 238 consecutive pediatric allogeneic HSCT recipients who underwent a single center were included prospective, weekly HAdV DNAemia monitoring program by quantitative PCR. loads >1000 copies/mL detected 15.5% of all patients. Despite low directly...
A human mastadenovirus D (HAdV-D) isolated from diarrhoeal faeces of an allogeneic haematopoietic stem cell transplant (SCT) recipient was found to be non-typable by sequencing loops 1 and 2 the hexon main neutralization epitope ('imputed serology'). In contrast HAdV-C, HAdV-D infections are rarely observed in SCT patients. Therefore, whole genome this isolate sequenced phylogenetically analysed. addition, microneutralization testing with type-specific antisera performed. complete genomic...
ABSTRACT Objective To examine the efficacy of hyperimmunoglobulin (HIG) treatment in women with a recent primary cytomegalovirus (CMV) infection up to 14 weeks' gestation. Methods This is an ongoing observational study conducted at prenatal medicine departments University Hospitals Tübingen, Bonn, Cologne and Erlangen, Germany, as well Laboratory Prof. Gisela Enders Colleagues Stuttgart, Germany Institute for Medical Virology Germany. Enrolment criteria were presence confirmed CMV first...
Varicella zoster virus (VZV) reactivation is a common infectious disease in neurology and VZV the second most frequent detected encephalitis. This study investigated characteristics of clinical laboratory features patients with infection.Two hundred eighty two that were hospitalized department time from 2005 to 2013 retrospectively evaluated. Results cerebrospinal fluid (CSF) analysis available 85 patients.Trigeminal rash was manifestation, followed by segmental rash, CNS infection, facial...
Although infections with the novel pandemic 2009 influenza A (H1N1) virus (A/H1N1/2009) appeared to be relatively mild during first summer of circulation (‘off season’), there has been significant morbidity and hospitalization several fatal cases. Thus, rapid detection A/H1N1/2009 is crucial for efficient treatment infection control measures. In contrast seasonal influenza, where point-of-care (POC) antigen tests direct fluorescent antibody (DFA) staining ensure detection, diagnosis so far...
Abstract Resolving the role of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission in households with members from different generations is crucial for containing current pandemic. We conducted a large-scale, multicenter, cross-sectional seroepidemiologic household study southwest Germany during May 11–August 1, 2020. included 1,625 participants 405 that each had ≥1 child and 1 reverse transcription PCR–confirmed SARS-CoV-2–infected index case-patient. The overall...
Abstract With viral infections, multiple related strains are often present due to coinfection or within-host evolution. We describe Haploflow, a deBruijn graph-based assembler for de novo genome assembly of from mixed sequence samples using novel flow algorithm. assess Haploflow across benchmark data sets increasing complexity, showing that is faster and more accurate than haplotype assemblers generic metagenome not aiming reconstruct strains. show reconstructs strain genomes patient HCMV...
Background: Blood contamination due to traumatic lumbar puncture presents a diagnostic pitfall in cerebrospinal fluid (CSF) analysis. It is controversially discussed if phagocytosis of erythrocytes which can be found the CSF after subarachnoid hemorrhage also develop vitro presence artificial blood contamination. Furthermore, there no consensus about acceptable amount on protein results. Methods: Two measurement series were performed order investigate role possible development erythrophages...
The aim of this study was to investigate the clinical and laboratory features adults with nervous system infections caused by enteroviruses, special emphasis on cerebrospinal fluid (CSF).The data 46 patients who were PCR-positive for enteroviruses in CSF between 2002 2017 evaluated.Meningitis most common manifestation (89%), followed encephalitis (7%) isolated cranial nerve involvement (4%). Twenty percent reported a sudden onset severe headache that led initial suspected diagnosis...
Significance Respiratory syncytial virus (RSV) remains a major cause of severe respiratory disease worldwide in children, older individuals, and immunosuppressed patients. An increasing understanding this burden together with the absence an effective vaccine limited therapeutic options has resulted many new preclinical clinical studies into novel vaccines, antibodies, antivirals. This requires complementary expansion RSV molecular toolkit to encompass reverse genetics systems based on...
Nonmelanoma skin cancer (NMSC) shows a strongly increased incidence in solid organ transplant recipients (OTRs) and AIDS patients, suggesting an infectious etiology. The role of certain viruses, i.e., cutaneous human papillomaviruses (HPVs), NMSC immunosuppressed patients remains controversial. Merkel cell polyomavirus (MCPyV), which was recently identified using high-throughput sequencing, has been linked to proliferations. Here, we aimed identify novel or known viral sequences at the...
Abstract With the establishment of routine virus load (DNAemia) screening for Human adenovirus (HAdV) and Cytomegalovirus (CMV) in post‐transplant care quality standards quantitative PCR‐assays are increasing. Established real‐time PCR assays were improved with a fully automated DNA‐extraction competitive internal control DNA packaged into lambda phage which serves as an extraction amplification each sample. HAdV CMV detected quantified simultaneously various types diagnostic samples like...