A5029932270- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Herpesvirus Infections and Treatments
- Lymphoma Diagnosis and Treatment
- Histiocytic Disorders and Treatments
- HIV Research and Treatment
- T-cell and Retrovirus Studies
- Polyomavirus and related diseases
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Complement system in diseases
- HIV/AIDS drug development and treatment
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- CNS Lymphoma Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Glycosylation and Glycoproteins Research
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
Medizinische Hochschule Hannover
2016-2025
Institute of Virology of the Slovak Academy of Sciences
2010-2024
German Center for Infection Research
2015-2024
Federal Institute for Risk Assessment
2022-2024
Allogene Therapeutics (United States)
2023
Hospices Civils de Lyon
2017-2023
Université Claude Bernard Lyon 1
2022
Deutsches Zentrum für Luft- und Raumfahrt e. V. (DLR)
2022
Hôpital de la Croix-Rousse
2022
Chongqing Electromechanical Holdings (China)
2022
Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human 8, may be the infectious cause of KS. Its prevalence in general population, on basis detection virus genome, is controversial. To investigate seroprevalence, we measured antibodies to a recombinant capsid-related (lytic cycle) KSHV antigen and latent complex.We selected potentially immunoreactive proteins by expressing them testing western blot assays. We used truncated protein encoded open reading frame 65 (orf 65) develop...
Kaposi's sarcoma (KS)-associated herpesvirus or human 8 (KSHV/HHV8) is the likely cause of KS and primary effusion lymphomas body cavity-based (BCBLs). A latency-associated nuclear immunofluorescence antigen (LANA) (D. H. Kedes, E. Operskalski, M. Busch, R. Kohn, J. Flood, D. Ganem, Nat. Med. 2:918-924, 1996; S. Gao, L. Kingsley, Li, W. Zheng, C. Parravicini, Ziegler, Newton, Rinaldo, A. Saah, Phair, Detels, Y. Chang, P. Moore, 2:925-928, 1996) a 222- to 234-kDa protein (LNA) (S. Hoover, T....
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
Certain infectious agents are recognised causes of cancer and other chronic diseases. To understand the pathological mechanisms underlying such relationships, here we design a Multiplex Serology platform to measure quantitative antibody responses against 45 antigens from 20 including human herpes, hepatitis, polyoma, papilloma, retroviruses, as well Chlamydia trachomatis, Helicobacter pylori Toxoplasma gondii, then assayed random subset 9695 UK Biobank participants. We find seroprevalence...
We studied the seroprevalence and transmission of Kaposi's sarcoma-associated herpesvirus (KSHV/HHV8), among 215 Ugandan children, adolescents young adults. measured antibodies to a latent nuclear antigen (LANA) lytic cycle protein encoded by open reading frame (orf) 65. Infection with KSHV/HHV8 occurred during early childhood reached adult levels (approx. 50%) before age puberty. In children younger than 12 years age, LANA orf65 were independently associated hepatitis B infection (p <...
Located close to the crown of V3 type-specific neutralization loop human immunodeficiency virus type 1 (HIV-1) (IIIB) SU glycoprotein gp120, are several potential sites that should be susceptible proteolytic cleavage by enzymes trypsinlike or chymotrypsinlike specificity, aspartic proteinases. The linkages potentially sensitive chymotryptic/aspartic proteinase retained also within equivalent domain HIV-2 (ROD) gp105. We show thrombin and tryptase cleave HTV-1 gp120 specifically at tryptic...
Human herpesvirus 8 (HHV-8) infection has been linked to the development of Kaposi's sarcoma and rare lymphoproliferative disorders.
A newly identified herpesvirus has been associated with Kaposi's sarcoma. We determined risk factors for Kaposi's-sarcoma-associated herpesvirus/human 8 (KSHV/HHV-8) seropositivity and incidence of infection over time in a cohort Danish homosexual men followed from 1981 to 1996. Antibodies latent nuclear (LANA) structural (orf65) antigen KSHV/HHV-8 were measured by immunofluorescence ELISA/WB respectively. Through linkage the national AIDS registry, all members diagnosed as September 1996...
Background: The finding of antibodies against human herpesvirus 8 (HHV-8) is associated with the occurrence Kaposi's sarcoma in persons infected HIV. However, predictive value HHV-8 for HIV infection unknown. Methods: Amsterdam Cohort Studies on and AIDS started 1984 homosexual men 1985 injecting drug users. Serum samples from 1459 1167 users were tested to recombinant lytic-phase capsid (ORF65) antigen latent-phase nuclear (ORF73) antigen. Individuals retrospectively identified as...
To standardize human herpesvirus 8 (HHV-8) antibody assays for application to asymptomatic infection, a blinded comparison was done of seven immunofluorescence and ELISAs. Five experienced laboratories tested serum panel from 143 subjects in 4 diagnostic groups. Except minor capsid protein ELISA, the other six tests detected HHV-8 antibodies most frequently classic (80%–100%) AIDS-related (67%–91%) Kaposi's sarcoma, followed by immunodeficiency virus-seropositive patients (27%–60%), least...
The impact of community-acquired respiratory virus (CARV) infections on bronchiolitis obliterans syndrome (BOS) and outcome after lung transplantation (LTx) diagnostic techniques were prospectively evaluated.A single-center prospective cohort study was performed in LTx-outpatients between October 31, 2005 April 30, 2006. Symptoms tract recorded nasopharyngeal oropharyngeal swabs obtained. Lower sampling when indicated. Immunofluorescence testing, cultures, polymerase chain reaction for 12...
The latency-associated nuclear antigen (LANA) of Kaposi sarcoma herpesvirus (KSHV) is mainly localized and functions in the nucleus latently infected cells, playing a pivotal role replication maintenance latent viral episomal DNA. In addition, N-terminally truncated cytoplasmic isoforms LANA, resulting from internal translation initiation, have been reported, but their function unknown. Using coimmunoprecipitation MS, we found cGMP-AMP synthase (cGAS), an innate immune DNA sensor, to be...
The inherent risks associated with vector insertion in gene therapy need to be carefully assessed. We analyzed the genome-wide distributions of Sleeping Beauty (SB) and piggyBac (PB) transposon insertions as well MLV retrovirus HIV lentivirus human CD4+ T cells respect a panel 40 chromatin states. distribution SB displayed least deviation from random, while PB showed unexpected parallels across all Both are enriched at transcriptional start sites (TSSs) co-localize BRD4-associated sites....
ABSTRACT Retroviral integrase (IN) proteins catalyze the permanent integration of proviral genomes into host DNA with help cellular cofactors. Lens epithelium-derived growth factor (LEDGF) is a cofactor for lentiviruses, including human immunodeficiency virus type 1 (HIV-1), and targets lentiviral toward active transcription units in genome. In contrast to murine leukemia (MLV), gammaretrovirus, tends integrate near start sites. Here, we show that bromodomain extraterminal domain (BET) BRD2,...