A5042492746- Virus-based gene therapy research
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Bladder and Urothelial Cancer Treatments
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- HIV Research and Treatment
- Cancer Immunotherapy and Biomarkers
- Viral gastroenteritis research and epidemiology
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Lymphoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Parvovirus B19 Infection Studies
- Prostate Cancer Treatment and Research
- Chromosomal and Genetic Variations
- Genetic factors in colorectal cancer
- T-cell and Retrovirus Studies
- Developmental Biology and Gene Regulation
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
University of Surrey
2015-2024
Target (United States)
2016
Vivex Biologics (United States)
2010
Cancer Research UK
2009
St James's University Hospital
2009
Oncolytics Biotech (Canada)
2009
Institute of Cancer Research
1992-2009
Hammersmith Hospital
2004-2006
Imperial College London
2004-2006
University of Liverpool
1996-2002
Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human 8, may be the infectious cause of KS. Its prevalence in general population, on basis detection virus genome, is controversial. To investigate seroprevalence, we measured antibodies to a recombinant capsid-related (lytic cycle) KSHV antigen and latent complex.We selected potentially immunoreactive proteins by expressing them testing western blot assays. We used truncated protein encoded open reading frame 65 (orf 65) develop...
Kaposi's sarcoma (KS)-associated herpesvirus or human 8 (KSHV/HHV8) is the likely cause of KS and primary effusion lymphomas body cavity-based (BCBLs). A latency-associated nuclear immunofluorescence antigen (LANA) (D. H. Kedes, E. Operskalski, M. Busch, R. Kohn, J. Flood, D. Ganem, Nat. Med. 2:918-924, 1996; S. Gao, L. Kingsley, Li, W. Zheng, C. Parravicini, Ziegler, Newton, Rinaldo, A. Saah, Phair, Detels, Y. Chang, P. Moore, 2:925-928, 1996) a 222- to 234-kDa protein (LNA) (S. Hoover, T....
A newly identified herpesvirus has been associated with Kaposi's sarcoma. We determined risk factors for Kaposi's-sarcoma-associated herpesvirus/human 8 (KSHV/HHV-8) seropositivity and incidence of infection over time in a cohort Danish homosexual men followed from 1981 to 1996. Antibodies latent nuclear (LANA) structural (orf65) antigen KSHV/HHV-8 were measured by immunofluorescence ELISA/WB respectively. Through linkage the national AIDS registry, all members diagnosed as September 1996...
Background: The finding of antibodies against human herpesvirus 8 (HHV-8) is associated with the occurrence Kaposi's sarcoma in persons infected HIV. However, predictive value HHV-8 for HIV infection unknown. Methods: Amsterdam Cohort Studies on and AIDS started 1984 homosexual men 1985 injecting drug users. Serum samples from 1459 1167 users were tested to recombinant lytic-phase capsid (ORF65) antigen latent-phase nuclear (ORF73) antigen. Individuals retrospectively identified as...
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ABSTRACT Kaposi’s sarcoma-associated herpesvirus (KSHV/HHV-8) is the likely infectious cause of sarcoma, primary effusion lymphoma, and some cases multicentric Castleman’s disease. Its latent nuclear antigen (LANA) expressed in nuclei latently infected cells may play a role persistence episomal viral DNA dividing cells. Here we report that LANA interacts with RING3, protein member Drosophila fsh (female sterile homeotic) family proteins, which have previously been implicated controlling gene...
Pseudotypes of gibbon ape leukemia virus/simian sarcoma-associated virus (GALV/SSAV) and feline subgroup B (FeLV-B) have been constructed by rescuing a Moloney murine vector genome with wild-type GALV/SSAV or FeLV-B. The resulting recombinant viruses utilized core envelope proteins from the conferred resistance to growth in L-histidinol upon infected cells virtue HisD gene encoded genome. They displayed host range specificity could be neutralized virus-specific antisera. Receptor...
Specific point mutations which affect viral tropism have been identified in both the V3 loop and CD4-binding region of human immunodeficiency virus type 1 surface glycoprotein gp120. Here we report that a single mutation first variable (V1) strain JRCSF is responsible for change tropism.
Abstract Purpose: To test combination treatment schedules of reovirus and cisplatin chemotherapy in human murine melanoma cell lines models to investigate the possible mechanisms synergistic antitumor effects. Experimental Design: The effects ± on vitro cytotoxicity viral replication were assessed using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay plaque assay. Interactions between agents by index analysis. Mode death was Annexin V/propidium...
Reovirus type 3 Dearing (T3D) has demonstrated oncolytic activity in vitro, vivo murine models and early clinical trials. However the true potential of viruses may only be realized fully combination with other modalities such as chemotherapy, targeted therapy radiotherapy. In this study, we examine reovirus T3D chemotherapeutic agents against human prostate cancer cell lines, particular focus on highly metastatic line PC3 agent docetaxel. Docetaxel is standard care for acts by disrupting...
As a clinical setting in which local live biological therapy is already well established, non-muscle invasive bladder cancer (NMIBC) presents intriguing opportunities for oncolytic virotherapy. Coxsackievirus A21 (CVA21) novel intercellular adhesion molecule-1 (ICAM-1)-targeted immunotherapeutic virus. This study investigated CVA21-induced cytotoxicity panel of human cell lines, revealing range sensitivities largely correlating with expression the viral receptor ICAM-1. CVA21 combination low...
The HOX genes are a family of transcription factors that help to determine cell and tissue identity during early development, which also over-expressed in number malignancies where they have been shown promote proliferation survival. purpose this study was evaluate the expression prostate cancer establish whether cells sensitive killing by HXR9, an inhibitor function. function inhibited using HXR9 peptide. gene assessed RNA extraction from or tissues followed quantitative PCR, siRNA used...
We have examined the human mammary tumor cell line T47D and found that these cells produce virus-like particles which band at typical density for retroviral on a sucrose gradient, possess reverse transcriptase activity, package HERV-K10-like sequences. Using this information bacterial expression system to identify long open reading frames, we identified individual clones full-length frames RNase H could encode activity detected in cells.
Abstract We have previously developed an oncolytic herpes simplex virus-1 based on a clinical virus isolate, which was deleted for ICP34.5 to provide tumor selected replication and ICP47 increase antigen presentation as well selective replication. A phase I/II trial using version of this expressing granulocyte macrophage colony-stimulating factor has shown promising results. The work reported here aimed develop in local control further increased through the combined expression highly potent...
The HOX genes are a family of homeodomain-containing transcription factors that determine cellular identity during development and which dys-regulated in some cancers. In this study we examined the expression oncogenic function mesothelioma, cancer arising from pleura or peritoneum is associated with exposure to asbestos.We tested sensitivity mesothelioma-derived lines MSTO-211H, NCI-H28, NCI-H2052, NCI-H226 HXR9, peptide antagonist protein binding its PBX co-factor. Apoptosis was measured...
LANA, the major latency-associated nuclear antigen of Kaposi's sarcoma herpesvirus/human herpesvirus-8 (KSHV/HHV-8), binds RING3 protein, one five human homologues fsh (female sterile homeotic) gene product Drosophila. In KSHV/HHV-8-infected cells LANA and viral episomes accumulate in heterochromatin-associated bodies. Here we show that several KSHV/HHV-8-negative cell lines derived from carcinomas, sarcomas lymphomas, was expressed at low levels, primarily localized to euchromatin,...
Abstract Background Tumour necrosis factor alpha (TNFα) therapy is a promising anti‐cancer treatment when combined with radiotherapy due to its potent radio sensitising effects, but systemic toxicity has limited clinical use. Previously, non‐replicative adenovirus vectors have been used deliver TNFα directly the tumour, including under control of radiation sensitive promoter. Here, we an ICP34.5 deleted, oncolytic herpes simplex virus (HSV) for delivery increase expression levels and spread...
The HOX genes encode a family of transcription factors that have key roles in both development and malignancy. Disrupting the interaction between proteins their binding partner, PBX, has been shown to cause apoptotic cell death range solid tumors. However, despite playing particularly significant role acute myeloid leukemia (AML), relationship gene expression patient survival not evaluated (with exception HOXA9), mechanism by which HOX/PBX inhibition induces this malignancy is well...
Prostate cancers are considered "cold" tumors characterized by minimal T cell infiltrates, absence of a type I interferon (IFN) signature, and the presence immunosuppressive cells. This non-inflamed phenotype is likely responsible for lack sensitivity prostate cancer patients to immune checkpoint blockade (ICB) therapy. Oncolytic virus therapy can potentially overcome this resistance immunotherapy in transforming cold into "hot," cell-infiltrated tumors. We investigated whether combination...
We have isolated a new foamy virus from blood samples taken two apparently healthy orangutans (Pongo pygmaeus). The older orangutan has since died with encephalopathy after brief acute illness, while the younger one, his grandson, remains well. These animals and 12 other had specific antibodies to as measured by immunofluorescence. antisera showed strong neutralization, only weak cross-reaction simian strains. Southern blotting gag env probes of human PCR amplification that virus, designated...