A5024133154- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- Viral-associated cancers and disorders
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Protein Tyrosine Phosphatases
- Salivary Gland Disorders and Functions
- Hepatitis B Virus Studies
- Viral Infections and Vectors
- Toxoplasma gondii Research Studies
- CRISPR and Genetic Engineering
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Evolution and Genetic Dynamics
- Hepatitis C virus research
- Galectins and Cancer Biology
- Poxvirus research and outbreaks
- Inflammasome and immune disorders
- RNA regulation and disease
- Plant Disease Resistance and Genetics
- Gene Regulatory Network Analysis
- Enterobacteriaceae and Cronobacter Research
- Rabies epidemiology and control
Institute for Respiratory Health
2019-2024
The University of Western Australia
2019-2024
Helmholtz Centre for Infection Research
2015-2020
The latency-associated nuclear antigen (LANA) of Kaposi sarcoma herpesvirus (KSHV) is mainly localized and functions in the nucleus latently infected cells, playing a pivotal role replication maintenance latent viral episomal DNA. In addition, N-terminally truncated cytoplasmic isoforms LANA, resulting from internal translation initiation, have been reported, but their function unknown. Using coimmunoprecipitation MS, we found cGMP-AMP synthase (cGAS), an innate immune DNA sensor, to be...
Article29 January 2019Open Access Source DataTransparent process The herpesviral antagonist m152 reveals differential activation of STING-dependent IRF and NF-κB signaling STING's dual role during MCMV infection Markus Stempel orcid.org/0000-0002-9240-3987 Viral Immune Modulation Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany Search more papers by this author Baca Chan Vanda Juranić Lisnić Center Proteomics, Faculty Medicine, University Rijeka, Croatia Astrid...
The type I interferon (IFN) response is imperative for the establishment of early antiviral immune response. Here we report identification first IFN antagonist encoded by murine cytomegalovirus (MCMV) that shuts down signaling following pattern recognition receptor (PRR) sensing. Screening an MCMV open reading frame (ORF) library identified M35 as a novel and strong negative modulator IFNβ promoter induction activation both RNA DNA cytoplasmic PRR. Additionally, inhibits proinflammatory...
The rapid activation of pattern recognition receptor (PRR)-mediated type I interferon (IFN) signaling is crucial for the host response to infection. In turn, human cytomegalovirus (HCMV) must evade this potent establish life-long Here, we reveal that HCMV tegument protein UL35 antagonizes IFN transcription downstream DNA and RNA sensors cGAS RIG-I, respectively. We show ectopic expression diminishes response, while infection with a recombinant lacking induces an elevated compared wildtype...
It is becoming increasingly clear that many diseases are the result of infection from multiple genetically distinct strains a pathogen. Such multi-strain infections have capacity to alter both disease and pathogen dynamics. Infection with human cytomegalovirus (HCMV) common has been linked enhanced disease. Suggestions enhancement in infected patients due complementation supported by trans-complementation studies mice during co-infection wild type gene knockout murine CMV (MCMV)....
Murine gammaherpesvirus 68 (MHV68) is a small-animal model suitable for study of the human pathogens Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. Here, we have characterized roles endosomal Toll-like receptor (TLR) escort protein UNC93B, TLR7, -9, -13, cell surface TLR2 in MHV68 detection. We found that alpha interferon (IFN-α) response plasmacytoid dendritic cells (pDC) to was reduced
Distinct cytomegaloviruses (CMVs) are widely distributed across their mammalian hosts in a highly host species-restricted pattern. To date, evidence demonstrating this has been limited largely to PCR-based approaches targeting small, conserved genomic regions, and only few complete genomes of isolated viruses representing distinct CMV species have sequenced. We now combined direct isolation infectious from tissues with genome sequencing provide view diversity wild animal population. targeted...
Spillover of infectious diseases from wild animal populations constitutes a long-standing threat to human health for which few globally viable solutions have been developed. The use oral baits laden with conventional vaccines distributed en masse represents one success story but is costly and practicable primarily rabies risk reduction in North American European carnivores. Efforts expand vaccination control pathogens within less accessible wildlife raised interest new kind vaccine capable...
Abstract Diverse applications rely on engineering microbes to carry and express foreign transgenes. This engineered baggage rarely benefits the microbe is thus prone rapid evolutionary loss when propagated. For where a transgene must be maintained for extended periods of growth, slowing rate evolution critical can achieved by reducing either mutation or strength selection. Because realized changing these quantities will not usually equal, it important know which yield greatest improvement...
The cloning of herpesviruses as bacterial artificial chromosomes (BACs) has revolutionized the study herpesvirus biology, allowing rapid and precise manipulation viral genomes. Several clinical strains human cytomegalovirus (HCMV) have been cloned BACs; however, no low-passage murine CMV (MCMV), which provide a model mimicking these isolates, cloned. Here, G4 strain was BAC carries an m157 gene that does not ligate natural killer (NK) cell-activating receptor, Ly49H, meaning unlike...
Protein tyrosine phosphatase 1B (PTP1B, also known as PTPN1) is a negative regulator of the leptin and insulin signalling pathways. This great interest PTP1B-knockout mice are protected against development obesity diabetes. Here, we provide evidence for novel function PTP1B that independent its activity, but requires localisation to membrane endoplasmic reticulum. Upon activation pattern recognition receptors, macrophages plasmacytoid dendritic cells from secrete lower amounts type I...
As the largest herpesviruses, 230 kb genomes of cytomegaloviruses (CMVs) have increased our understanding host immunity and viral escape mechanisms, although many annotated genes remain as yet uncharacterised. Here we identify m15 locus murine CMV (MCMV) a modulator natural killer (NK) cell immunity. We show that, rather than discrete transcripts from m14, m16 annotated, there are five 3′-coterminal expressed over this region, all utilising consensus polyA tail at end gene. Functional...
The potential infectivity of 1129 randomly selected plasma specimens was directly assayed by hepatitis B virus (HBV) DNA dot-hybridization. Presence or absence HBV then correlated with a biochemical profile 20 common analytes obtained on these same specimens. We found that potentially infectious could not be identified the basis any combination simple tests; indeed, were more "normal" in some tests liver function than non-infectious