A5019787454- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Molecular Sensors and Ion Detection
- Genomics and Phylogenetic Studies
- Crystallography and molecular interactions
- Cytomegalovirus and herpesvirus research
- Protease and Inhibitor Mechanisms
- Luminescence and Fluorescent Materials
- RNA and protein synthesis mechanisms
- Porphyrin and Phthalocyanine Chemistry
- Crystal structures of chemical compounds
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Herpesvirus Infections and Treatments
- RNA modifications and cancer
- Supramolecular Chemistry and Complexes
- Blood properties and coagulation
- Mycobacterium research and diagnosis
- Plant Gene Expression Analysis
- Viral-associated cancers and disorders
- Plant biochemistry and biosynthesis
- Gene expression and cancer classification
- Cell Adhesion Molecules Research
- Enzyme Production and Characterization
- Single-cell and spatial transcriptomics
- Metal complexes synthesis and properties
Biospyder (United States)
2023-2024
MRC University of Glasgow Centre for Virus Research
2018-2023
Medical Research Council
2019-2023
University of Sassari
2009-2019
The University of Texas at Austin
2003-2008
University of Southampton
2000-2003
Roswell Park Comprehensive Cancer Center
1981-2000
New York State Department of Health
1980-1987
Kaleida Health
1981
SummaryIn the presence of porcine tissue plasminogen activator, fibrinolysis is greatly enhanced in comparison with fibrinogenolysis. In contrast, other activators (UK, SK-activator) and plasmin showed no, or moderate, differences between lysis fibrinogen fibrin. The activator effect related to activation plasminogen. observations add a specific mechanism enhancement by that caused influence inhibitors. process may help enhance localized following injury without affecting fibrinogen.
Two new pyrrole 2,5-diamide clefts have been synthesized containing 4-nitrophenyl or 3,5-dinitrophenyl groups appended to the amide positions. The derivative has shown deprotonate in presence of fluoride, which acetonitrile solution, gives rise a deep blue colour.
Abstract The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total 207 datasets generated in this previous studies using target enrichment high-throughput sequencing analyzed, the process enabling determination genome sequences for 91 strains. Key findings that (i) it is important to monitor quality libraries investigating...
ABSTRACT oriP is a 1.7-kb region of the Epstein-Barr virus (EBV) chromosome that supports replication and stable maintenance plasmids in human cells. contains two essential components, called DS FR, both which contain multiple binding sites for EBV-encoded protein, EBNA-1. The appears to function as replicator , while FR acts conjunction with EBNA-1 prevent loss from proliferating Because EBNA-1's role stabilizing through it has not been entirely clear what extent might be required per se,...
Plasminogen activator content was determined quantitatively in extracts of 23 pairs surgically removed colon tumors and adjacent normal mucosa specimens. The averaged 4.4 times higher the tumor samples than corresponding tissue. Polyps with adenocarcinomas gave values intermediate between those for mucosae. enzyme group that showed invasive propagation or metastatic spread significantly (P<0.05) not manifesting these conditions. Activator activity completely inhibited by rabbit antibody...
3,4-Dichoro-1H-pyrrole-2,5-dicarboxylic acid bis-phenylamide 3 and 3,4-dichoro-1H-pyrrole-2,5-dicarboxylic bis-butylamide 4 have been prepared shown to deprotonate in the presence of basic anions: X-ray crystal structure tetrabutylammonium salt 3-H+ reveals formation a dimer solid state.
Two molecules containing two 2,5-diamido,3,4-dichloropyrrole units linked via 1,3- or 1,4-substituted benzene rings have been synthesized. The pyrrole groups in these compounds deprotonate the presence of tetrabutylammonium fluoride and form anion dimers NH···N- hydrogen bonds. This dimerization process results formation polyanionic chains an unusual "orthogonal" hydrogen-bonding array.
A new "super-extended cavity" tetraacetylcalix[4]pyrrole derivative was synthesized and characterized, X-ray crystal structures of complexes bound to fluoride acetonitrile were obtained. The binding behavior this receptor investigated by NMR titration, the complex found exclusively bind ions in DMSO-d6. This unusual Monte Carlo free energy perturbation simulations Poisson calculations, ion specificity seen result from favorable electrostatic interactions that gains sitting lower phenolic...
Extended cavity ester (4) and amide (5) calix[4]pyrrole macrocycles have been synthesised shown to bind fluoride exclusively in deuteriated DMSO solution.
Background Congenital cytomegalovirus disease (cCMV) can be severe but is uncommon. Investigations of the role genome sequence variation in causative virus (human cytomegalovirus, HCMV) clinical outcome have to date depended on small sample numbers derived from fresh tissues. Extensive formalin-fixed, paraffin-embedded (FFPE) cCMV biorepositories established worldwide potentially provide much larger for future investigations. However, there are no published reports sequencing whole HCMV...
Four new 2-amidopyrroles and 2,5-diamidopyrroles have been synthesized their anion complexation properties investigated. The crystal structures of these receptors elucidated reveal hydrogen bonding in the solid state leading to dimer network formation. Selectivity for oxo-anions has demonstrated by (1)H NMR titration techniques.
Abstract The plasminogen activator content of the extracts excised prostate cancers (25 specimens) was determined with an azocasein assay and found to be on average 1.7 times higher than that benign hyperplasias (29 specimens). Both groups contained same percentage human urokinase type (∼45%) as by inhibition activity when anti‐human antibody included in system. two types activators were partially purified have distinctly different properties. most striking difference large augmentation...
Quantitation of plasminogen activators present in tissue may depend to a large extent on the extraction procedure used solubilize enzymes. Potassium thiocyanate solution is known be an efficient solubilizer, but it can inhibit assay systems other than fibrin plates. An equally effective acetate-detergent extractant reported here which with highly sensitive azocaseinolytic procedure. The results indicate that three-fold increase activator activity extracted from selected tissues relative...
The codon composition of coding sequences plays an important role in the regulation gene expression. Herein, we report systematic differences usage synonymous codons among Arabidopsis thaliana genes that are expressed specifically distinct tissues. Although observed both regionally and transcriptionally associated mutational biases were significantly with bias, they could not explain fully. Similarly, given transcript abundances did account for usage, it is unlikely selection translational...
In developed countries, human cytomegalovirus (HCMV) is a major pathogen in congenitally infected and immunocompromised individuals, where multiple-strain infection appears linked to disease severity. The situation less documented developing countries. Zambia, breast milk key route for transmitting HCMV carries higher viral loads immunodeficiency virus (HIV)-infected women. We investigated strain diversity.High-throughput sequence datasets were generated from 28 HCMV-positive samples donated...
Codon bias is the non-random use of synonymous codons, a phenomenon that has been observed in species as diverse bacteria, plants and mammals. The preferential particular codons may reflect neutral mechanisms (e.g. mutational bias, G|C-biased gene conversion, genetic drift) and/or selection for mRNA stability, translational efficiency accuracy. extent to which these different factors influence codon usage unknown, so we dissected contribution towards genes from 15 eudicots, 4 monocots 2...
A molecular approach was applied to the study of carotenoid biosynthetic pathway Rhodotorula mucilaginosa. At first, functional annotation genome R. mucilaginosa C2.5t1 carried out and gene ontology categories were assigned 4033 predicted proteins. Then, a set genes involved in different steps carotenogenesis identified those coding for phytoene desaturase, synthase/lycopene cyclase dioxygenase (CAR genes) proved be clustered within region ~10 kb. Quantitative PCR biosynthesis showed that...