A5012568958- Platelet Disorders and Treatments
- Blood groups and transfusion
- Blood disorders and treatments
- Heparin-Induced Thrombocytopenia and Thrombosis
- Cell Adhesion Molecules Research
- Blood transfusion and management
- Immunodeficiency and Autoimmune Disorders
- Hemophilia Treatment and Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Venous Thromboembolism Diagnosis and Management
- Erythrocyte Function and Pathophysiology
- Intramuscular injections and effects
- Blood Coagulation and Thrombosis Mechanisms
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Monoclonal and Polyclonal Antibodies Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood properties and coagulation
- Hemoglobinopathies and Related Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Chronic Myeloid Leukemia Treatments
- Blood donation and transfusion practices
- Atrial Fibrillation Management and Outcomes
- Systemic Lupus Erythematosus Research
- Neutropenia and Cancer Infections
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
University of Giessen
2016-2025
Universitätsklinikum Gießen und Marburg
2014-2025
Immungenetics (Germany)
2025
Universities of Giessen and Marburg Lung Center
2013-2024
Leiden University Medical Center
2024
Institute of Immunology
2021
Philipps University of Marburg
2013-2019
University of Freiburg
2017
Universitas Gadjah Mada
2016
University of Rostock
2000-2016
The recently described junctional adhesion molecules (JAMs) in man and mice are involved homotypic heterotypic intercellular interactions. Here, a third member of this family, human JAM-3, was identified as novel counterreceptor on platelets for the leukocyte β2-integrin Mac-1 (αMβ2, CD11b/CD18). With help two monoclonal antibodies, Gi11 Gi13, against 43-kD surface glycoprotein platelets, full-length cDNA encoding JAM-3 identified. is type I transmembrane containing Ig-like domains. Although...
The COVID-19 pandemic has resulted in significant morbidity and mortality worldwide. To prevent severe infection, mass vaccination campaigns with several vaccine types are currently underway. We report pathological immunological findings 8 patients who developed vaccine-induced immune thrombotic thrombocytopenia (VITT) after administration of SARS-CoV-2 ChAdOx1 nCoV-19. analyzed patient material using enzyme assays, flow cytometry heparin-induced platelet aggregation assay performed...
In early-onset severe hemolytic disease of the fetus and newborn (HDFN), transplacental transfer maternal antierythrocyte IgG alloantibodies causes fetal anemia that leads to use high-risk intrauterine transfusions in order avoid hydrops death. Nipocalimab, an anti-neonatal Fc receptor blocker, inhibits lowers levels.
Human neutrophil-specific CD177 (NB1 and PRV-1) has been reported to be up-regulated in a number of inflammatory settings, including bacterial infection granulocyte-colony-stimulating factor application. Little is known about its function. By flow cytometry immunoprecipitation studies, we identified platelet endothelial cell adhesion molecule-1 (PECAM-1) as binding partner CD177. Real-time protein-protein analysis using surface plasmon resonance confirmed cation-dependent, specific...
The third member of the family junctional adhesion molecules (JAMs), JAM-3, also called JAM-C, was recently shown to be a novel counter-receptor on platelets for leukocyte beta(2)-integrin Mac-1 (alphaMbeta(2), CD11b/CD18). Here, new functional aspects role endothelial cell JAM-C were investigated. Endothelial cells express which is predominantly localized within junctions at interendothelial contacts, since it codistributes with tight junction component, zonula occludens-1. Whereas does not...
It is commonly accepted that antibody-mediated removal of platelets represents a major mechanism platelet destruction in immune thrombocytopenic purpura (ITP). Although complement activation may participate clearance, frequency and specificity have not yet been studied systematically ITP.We examined blood samples from 240 patients with ITP. Samples were assessed for the presence free bound autoantibodies by standard glycoprotein-specific assay (monoclonal antibody-specific immobilization...
Objective— Fetal/neonatal alloimmune thrombocytopenia is a severe bleeding disorder, which can result in intracranial hemorrhage (ICH), leading to death or neurological sequelae. In whites, maternal anti–human platelet antigen-1a (HPA-1a) antibodies are responsible for the majority of cases. No predictive factors ICH available guide prophylactic treatment during pregnancy. this study, we investigated from mothers with ICH-positive fetal/neonatal and ICH-negative identify serological...
Summary The neonatal Fc receptor, FcRn, plays a central role in immunoglobulin G (IgG) transport across placental barriers. Genetic variations of FcRn‐dependent the placenta may influence antibody‐mediated pathologies fetus and newborn. Sequencing analysis 20 unrelated individuals demonstrated no missense mutation within five exons FcRn gene. However, variable number tandem repeats (VNTR) region promoter was observed, consisting different alleles (VNTR1–VNTR5). Alleles with two (VNTR2) three...
BACKGROUND: Bacterial and fungal infections in profound neutropenia after chemotherapy are associated with high mortality despite appropriate antibacterial antifungal treatment. Granulocyte transfusions used as a therapeutic addendum, but concern regarding pulmonary reactions often results delayed use clinical practice. Accordingly, many patients already at advanced stages of their infectious disease once granulocytes transfused. Thus, prospective Phase II trial was conducted to test the...
The human neutrophil-specific adhesion molecule CD177 (also known as the NB1 alloantigen) becomes upregulated on cell surface in a number of inflammatory settings. We recently showed that functions novel heterophilic counterreceptor for endothelial junctional protein PECAM-1 (CD31), an interaction is mediated by membrane-proximal IgD 6, which to harbor S(536)N single nucleotide polymorphism two major isoforms V(98)N(536)G(643) and L(98)S(536)R(643) yet-to-be-determined region CD177. In vitro...
Antibodies against human neutrophil antigen-3a (HNA-3a) located on choline transporter-like protein 2 induce severe transfusion-related acute lung injury (TRALI). This study aims to identify the mechanism implicated in anti-HNA-3a-mediated TRALI.Our analysis shows that anti-HNA-3a recognizes isoforms (P1 and P2) microvascular endothelial cells from blood vessels but reacts only with P1 isoform neutrophils. Direct treatment of HNA-3a-positive anti-HNA-3a, not anti-HNA-3b, leads reactive...