A5066969008- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Mechanisms of cancer metastasis
- Polyamine Metabolism and Applications
- Pluripotent Stem Cells Research
- Protease and Inhibitor Mechanisms
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Protein Kinase Regulation and GTPase Signaling
- Endoplasmic Reticulum Stress and Disease
- 3D Printing in Biomedical Research
- Enzyme function and inhibition
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Tissue Engineering and Regenerative Medicine
- Phagocytosis and Immune Regulation
- Plant Reproductive Biology
- Ion channel regulation and function
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Calcium signaling and nucleotide metabolism
Institut Curie
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Sorbonne Université
2010-2025
Université Paris Sciences et Lettres
2015-2024
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2021-2024
Cytoskeleton (United States)
2013-2023
Université Paris Cité
2005-2021
Dynamique du noyau
2010-2020
Laboratoire de Biologie et Modélisation de la Cellule
2020
University of Birmingham
2013
A set of 11 clones encoding putative GTP binding proteins highly homologous to the yeast YPT1/SEC4 gene products have been isolated from an MDCK cell cDNA library. We localized three corresponding in mammalian cells by using affinity-purified antibodies immunofluorescence and immunoelectron microscopy studies. One, homolog rab2, is associated with a structure having characteristics intermediate compartment between endoplasmic reticulum Golgi apparatus. The second, rab5, located at...
Using an original microfabrication-based technique, we experimentally study situations in which a virgin surface is presented to confluent epithelium with no damage made the cells. Although inspired by wound-healing experiments, situation markedly different from classical scratch wounding because it focuses on influence of free and uncouples other possible contributions such as cell and/or permeabilization. Dealing Madin-Darby canine kidney cells various surfaces, found that sudden release...
We measure dynamic traction forces exerted by epithelial cells on a substrate. The force sensor is high-density array of elastomeric microfabricated pillars that support the cells. Traction induced cell migration are deduced from measurement bending these and correlated with actin localization fluorescence microscopy. use multiple-particle tracking method to estimate mechanical activity in real time high-spatial resolution (down 2 μm) imposed periodicity post array. For experiments, we...
The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from melanogaster , most them orthologous cancer-related and/or signaling proteins, against high-complexity fly cDNA libraries. More than 2300 protein-protein interactions (PPI) were identified, which 710 are high confidence. computation reliability score for...
Cancer cells use different modes of migration, including integrin-dependent mesenchymal migration elongated along elements the 3D matrix as opposed to low-adhesion-, contraction-based amoeboid motility rounded cells. We report that MDA-MB-231 human breast adenocarcinoma invade Matrigel with a characteristic morphology and F-actin myosin-IIa accumulating at cell rear in uropod-like structure. display neither lamellipodia nor bleb extensions leading edge do not require Arp2/3 complex activity...
Invadopodia are actin-based membrane protrusions formed at contact sites between invasive tumor cells and the extracellular matrix with proteolytic activity. Actin regulatory proteins participate in invadopodia formation, whereas degradation requires metalloproteinases (MMPs) targeted to invadopodia. In this study, we show that vesicle-tethering exocyst complex is required for proteolysis invasion of breast carcinoma cells. We demonstrate subunits Sec3 Sec8 interact polarity protein IQGAP1...
Ewing sarcoma is characterized by the expression of chimeric EWSR1-FLI1 transcription factor. Proteomic analyses indicate that decrease leads to major changes in effectors dynamics actin cytoskeleton and adhesion processes with a shift from cell-to-cell cell-matrix adhesion. These are associated dramatic increase vivo cell migration invasion potential. Importantly, expression, evaluated single-cell RT-ddPCR/immunofluorescence analyses, activity, assessed downstream targets, heterogeneous...
ABSTRACT Rab GTPases belong to the Ras GTPase superfamily and are key regulators of membrane traffic. Among them, rab7 has been localized on late endosomes NRK cells but its function remains unknown. In order investigate role, we generated stable HeLa cell lines that express either wild type or a GTPase-defective mutant in an inducible manner. A morphological analysis intracellular localization these proteins was performed by confocal laser microscopy. Here show that, cells, is present...
The Rho GTPases play a critical role in initiating actin polymerization during phagocytosis. In contrast, the factors directing disassembly of F-actin required for fission phagocytic vacuole are ill defined. We used fluorescent chimeric proteins to monitor dynamics association and active Cdc42 Rac1 with forming phagosome. Although was found disappear from base phagosome before sealing complete, Rac1/Cdc42 activity persisted, suggesting that termination GTPase is not main determinant...
The small guanosine triphosphate (GTP)-binding protein ADP-ribosylation factor (ARF) 6 regulates membrane recycling to regions of plasma remodeling via the endocytic pathway. Here, we show that GTP-bound ARF6 interacts with Sec10, a subunit exocyst complex involved in docking vesicles membrane. We found Sec10 localization perinuclear region is not restricted trans-Golgi network, but extends endosomes. In addition, report depletion Sec5 or dominant inhibition affects function and morphology...
Molecular analysis of Saccharomyces cerevisiae secretion mutants has led to the identification two Ras-like GTP-binding proteins, Ypt1p and Sec4p, which are essential for transport along exocytic route. To study regulation membrane traffic in epithelial cells, a set 11 clones encoding proteins similar YPT1/SEC4 products were isolated from an MDCK (Madin-Darby canine kidney) cell cDNA library. Four these Rab8, -9, -10, -11, novel members this subfamily them closely related Sec4p. The ratio...