A5065550629- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
- Cellular Mechanics and Interactions
- Neurogenesis and neuroplasticity mechanisms
- Cellular transport and secretion
- Nerve injury and regeneration
- Axon Guidance and Neuronal Signaling
- Neurological disorders and treatments
- Parkinson's Disease Mechanisms and Treatments
- Hereditary Neurological Disorders
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Protein Tyrosine Phosphatases
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Signaling Pathways in Disease
- Cannabis and Cannabinoid Research
- Adipose Tissue and Metabolism
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
Institut du Fer à Moulin
2016-2025
Inserm
2016-2025
Sorbonne Université
2016-2025
Centre National de la Recherche Scientifique
2003-2025
Institut du Cerveau
2025
Institut National de Recherche en Santé Publique
2005-2023
Sorbonne University Abu Dhabi
2019-2022
Sorbonne Paris Cité
2020
Délégation Paris 6
2020
Université Sorbonne Nouvelle
2019
Many drugs of abuse exert their addictive effects by increasing extracellular dopamine in the nucleus accumbens, where they likely alter plasticity corticostriatal glutamatergic transmission. This mechanism implies key molecular alterations neurons which both and glutamate inputs are activated. Extracellular signal-regulated kinase (ERK), an enzyme important for long-term synaptic plasticity, is a good candidate playing such role. Here, we show mouse that d -amphetamine activates ERK subset...
Psychostimulants and other drugs of abuse activate extracellular signal-regulated kinase (ERK) in the striatum, through combined stimulation dopamine D 1 receptors (D1Rs) glutamate NMDA receptors. Antipsychotic similar signaling proteins striatum by blocking 2 (D2Rs). However, neurons which these pathways are activated psychotropic not precisely identified. We used transgenic mice, enhanced green fluorescent protein (EGFP) expression was driven D1R promoter ( drd1a -EGFP) or D2R drd2 -EGFP)....
The Drosophila (fruit fly) model system has been instrumental in our current understanding of human biology, development, and diseases. Here, we used a high-throughput yeast two-hybrid (Y2H)-based technology to screen 102 bait proteins from melanogaster , most them orthologous cancer-related and/or signaling proteins, against high-complexity fly cDNA libraries. More than 2300 protein-protein interactions (PPI) were identified, which 710 are high confidence. computation reliability score for...
Dopaminergic neurons exert a major modulatory effect on the forebrain. Dopamine and adenosine 3′,5′-monophosphate–regulated phosphoprotein (32 kilodaltons) (DARPP-32), which is enriched in all that receive dopaminergic input, converted response to dopamine into potent protein phosphatase inhibitor. Mice generated contain targeted disruption of DARPP-32 gene showed profound deficits their molecular, electrophysiological, behavioral responses dopamine, drugs abuse, antipsychotic medication....
Abstract A major goal of research on addiction is to identify the molecular mechanisms long‐lasting behavioural alterations induced by drugs abuse. Cocaine and delta‐9‐tetrahydrocannabinol (THC) activate extracellular signal‐regulated kinase (ERK) in striatum blockade ERK pathway prevents establishment conditioned place preference these drugs. However, it not known whether activation specific for two and/or this brain region. We studied appearance phospho‐ERK immunoreactive neurons CD−1...
The molecular basis of l-3,4-dihydroxyphenylalanine (l-DOPA)-induced dyskinesia (LID), one the major hindrances in current therapy for Parkinson9s disease, is still unclear. We show that attenuation cAMP signaling medium spiny neurons striatum, achieved by genetic inactivation dopamine and cAMP-regulated phosphoprotein 32 kDa (DARPP-32), reduces LID. also that, dyskinetic mice, sensitized cAMP/cAMP-dependent protein kinase/DARPP-32 leads to phosphorylation/activation extracellular...
Alcohol consumption is a moderately heritable trait, but the genetic basis in humans largely unknown, despite its clinical and societal importance. We report genome-wide association study meta-analysis of ∼2.5 million directly genotyped or imputed SNPs with alcohol (gram per day kilogram body weight) among 12 population-based samples European ancestry, comprising 26,316 individuals, replication genotyping an additional 21,185 individuals. SNP rs6943555 autism susceptibility candidate 2 gene...
It has been well documented that neurotrophins, including brain-derived neurotrophic factor (BDNF), are severely affected in Alzheimer9s disease (AD), but their administration faces a myriad of technical challenges. Here we took advantage the early astrogliosis observed an amyloid mouse model AD (5xFAD) and used it as internal sensor to administer BDNF conditionally locally. We first demonstrate relevance release from astrocytes by evaluating effects coculturing WT neurons BDNF-deficient...
Endocannabinoids form a novel class of intercellular messengers, the functions which include retrograde signaling in brain and mediation or modulation several types synaptic plasticity. Yet, mechanisms long-term effects stimulation CB1 cannabinoid receptors (CB1-R) are poorly understood. We show that anandamide, 2-arachidonoyl-glycerol, Δ9-tetrahydrocannabinol (THC) activated extracellular signal-regulated kinase (ERK) hippocampal slices. In living mice, THC ERK neurons induced its...
Repeated association of drugs abuse with context leads to long-lasting behavioral responses that reflect reward-controlled learning and participate in the establishment addiction. Reactivation consolidated memories is known produce a reconsolidation process during which undergo labile state. We investigated whether reexposure had similar effects. Cocaine administration activates extracellular signal-regulated kinase (ERK) striatum, ERK activation required for acquisition cocaine-induced...
Although the induction of persistent behavioral alterations by drugs abuse requires regulation gene transcription, precise intracellular signaling pathways that are involved remain mainly unknown. Extracellular signal-regulated kinase (ERK) is critical for expression immediate-early genes in striatum response to cocaine and Δ9-tetrahydrocannabinol rewarding properties these drugs. Here we show mice a single injection (10 mg/kg) activates mitogen- stress-activated protein 1 (MSK1) dorsal...
Mice incapable of synthesizing the abundant galactolipids myelin exhibit disrupted paranodal axo-glial interactions in central and peripheral nervous systems. Using these mutants, we have analyzed role that play establishment axonal protein distribution region node Ranvier. Whereas clustering nodal proteins, sodium channels, ankyrinG, neurofascin was only slightly affected, potassium channels paranodin, proteins are normally concentrated regions juxtaposed to node, dramatically altered. The...
Using specific antibodies and cDNA probes, we have investigated, in rat basal ganglia, the distribution regulation of expression alpha subunits Gs G(olf), two GTP-binding proteins (G-proteins) that stimulate adenylyl cyclase. We confirmed G(olf) is highly expressed caudate-putamen, nucleus accumbens, olfactory tubercle, whereas less abundant these areas than other brain regions. Intrastriatal injections quinolinic acid decreased dramatically levels protein striatum substantia nigra, those...
Precise identification of neuronal populations is a major challenge in neuroscience. In the striatum, more than 95% neurons are GABAergic medium-sized spiny (MSNs), which form two intermingled distinguished by their projections and protein content. Those expressing dopamine D(1)-receptors (D1Rs) project preferentially to substantia nigra pars reticulata (SNr), whereas those D(2)- receptors (D2Rs) lateral part globus pallidus (LGP). The degree segregation these has been continuous subject...