A5033885078- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Genetic Neurodegenerative Diseases
- Cell Adhesion Molecules Research
- Nuclear Receptors and Signaling
- Genetics and Neurodevelopmental Disorders
- Cellular Mechanics and Interactions
- Protein Kinase Regulation and GTPase Signaling
- Neurogenesis and neuroplasticity mechanisms
- Microtubule and mitosis dynamics
- Histone Deacetylase Inhibitors Research
- Adipose Tissue and Metabolism
- Biochemical and Structural Characterization
- Retinal Development and Disorders
- Epigenetics and DNA Methylation
- Botulinum Toxin and Related Neurological Disorders
- Cellular transport and secretion
- Congenital heart defects research
- Laser Applications in Dentistry and Medicine
- Apelin-related biomedical research
- Dermatologic Treatments and Research
- Neural dynamics and brain function
- Connective tissue disorders research
- Neuroendocrine regulation and behavior
AbbVie (United States)
2022
Allergan (United States)
2022
University of California, Irvine
2014-2020
Sorbonne Université
2006-2020
Inserm
2007-2020
Institut du Fer à Moulin
2006-2020
Centre National de la Recherche Scientifique
2002-2009
Unit of Functional and Adaptive Biology
2006
Although the induction of persistent behavioral alterations by drugs abuse requires regulation gene transcription, precise intracellular signaling pathways that are involved remain mainly unknown. Extracellular signal-regulated kinase (ERK) is critical for expression immediate-early genes in striatum response to cocaine and Δ9-tetrahydrocannabinol rewarding properties these drugs. Here we show mice a single injection (10 mg/kg) activates mitogen- stress-activated protein 1 (MSK1) dorsal...
Akt is classically described as a prosurvival serine/threonine kinase activated in response to trophic factors. After activation by phosphoinositide 3-kinase (PI3-kinase), it can translocate the nucleus where promotes specific genetic programs catalyzing phosphorylation of transcription We report here that both dopamine (DA) D1 (SKF38393) and D2 (quinpirole) agonist treatments rapidly increase, primary striatal neurons culture, levels on Thr(308), residue critically involved its activity....
The extracellular signal-regulated kinases (ERKs) 1/2 pathway is stimulated by drugs of abuse in striatal neurons through coincident activation dopamine D 1 and glutamate NMDA receptors critical for long-lasting behavioral effects these drugs. Although regulation transcription a major target ERK, the precise mechanisms which it contributes to alterations not known. We examined role Zif268 , an immediate-early gene induced under control responses cocaine using knock-in mutant mice was...
Huntington's disease (HD) is a neurodegenerative disorder due to an abnormal polyglutamine expansion in the N-terminal region of huntingtin protein (Exp-Htt). This causes aggregation and neuronal dysfunction death. Transcriptional dysregulation Exp-Htt participates death HD. Here, using R6/2 transgenic mouse model HD, we identified new molecular alteration that could account for gene these mice. Despite nuclear activation mitogen-activated kinase/extracellular regulated kinase (ERK) along...
The dopamine D2 receptor (D2R) is a major component of the system. D2R-mediated signaling in neurons involved presynaptic regulation levels. Postsynaptically, i.e., striatal neurons, D2R controls complex functions such as motor activity through cell firing and heterologous neurotransmitter release. presence two isoforms, D2L D2S, which are generated by mechanism alternative splicing Drd2 gene, raises question whether both isoforms may equally control postsynaptic events. Here, we addressed...
Abstract Chromatin remodelling is thought to play a key role in gene regulation that underlies long‐term synaptic plasticity and memory formation. The dynamic process of chromatin requires post‐translational modifications histones, group highly basic proteins are tightly linked DNA. In the present study, we investigated histone H3 response glutamate stimulation leading c‐Fos c‐Jun induction an vitro model system striatal neurons culture. Intracellular signalling pathways implicated these...
Abstract Substance abuse disorders are linked to alteration of circadian rhythms, although the molecular and neuronal pathways implicated have not been fully elucidated. Addictive drugs, such as cocaine, induce a rapid increase dopamine levels in brain. Here, we show that acute administration cocaine triggers reprogramming gene expression striatum, an area involved psychomotor rewarding effects drugs. This process involves activation peroxisome protein activator receptor gamma (PPARγ),...
Abstract The botulinum neurotoxin serotype A (BoNT/A) cuts a single peptide bond in SNAP25, an activity used to treat wide range of diseases. Reengineering the substrate specificity BoNT/A’s protease domain (LC/A) could expand its therapeutic applications; however, LC/A’s extended recognition (≈ 60 residues) challenges conventional approaches. We report directed evolution method for retargeting LC/A and retaining exquisite specificity. resultant eight-mutation (omLC/A) has improved cleavage...
Permanent tagging of neuronal ensembles activated in specific experimental situations is an important objective to study their properties and adaptations. In the context learning memory, these neurons are referred as engram neurons. Here, we describe characterize a novel mouse line, Egr1-CreERT2 , which carries transgene promoter immediate early gene Egr1 drives expression CreERT2 recombinase that only active presence tamoxifen metabolite, 4-hydroxy-tamoxifen (4-OHT). mice were crossed with...
Excess sebum (seborrhea) results in oily skin and is associated with large pore size acne. Studies healthy, seborrheic volunteers have reported that intradermal injection of commercial preparations botulinum neurotoxin type A (BoNT/A) (onabotulinumtoxinA, abobotulinumtoxinA, incobotulinumtoxinA) reduced production, thus, oiliness size. The mechanism for these effects has not been fully elucidated; however, several theories involving direct or indirect BoNT/A on neuronal and/or dermal cells...
Abstract Permanent tagging of neuronal ensembles activated in specific experimental situations is an important objective to study their properties and adaptations. In the context learning memory these neurons are referred as engram neurons. Here we describe characterize a novel mouse line, Egr1-CreER T2 , which carries transgene promoter immediate early gene Egr1 drives expression CreER recombinase that only active presence tamoxifen metabolite, 4-hydroxy-tamoxifen (4-OHT). mice were crossed...
<h3></h3> Transcriptional dysregulation has emerged as a pathogenic process that appears early in Huntington’s disease (HD) progression and been recapitulated across multiple HD models. We have studying the involvement of Mitogen Stress-activated Kinase-1 (MSK-1) transcriptional HD. This striatum-enriched protein kinase acts on chromatin remodelling through histone H3 phosphorylation leading to activation. MSK-1 plays dual role gene transcription since it also activates factor...