A5014206766- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Retinoids in leukemia and cellular processes
- RNA regulation and disease
- Virus-based gene therapy research
- Effects and risks of endocrine disrupting chemicals
- Signaling Pathways in Disease
- Nuclear Structure and Function
- Sarcoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Health, Environment, Cognitive Aging
- Genetics and Neurodevelopmental Disorders
- interferon and immune responses
- Calpain Protease Function and Regulation
- Advanced biosensing and bioanalysis techniques
Institut de Recherche en Santé, Environnement et Travail
2021-2023
École des Hautes Études en Santé Publique
2021-2023
Inserm
2007-2023
Centre National de la Recherche Scientifique
1999-2023
Université de Rennes
2008-2023
Institut de génétique et de développement de Rennes
2012-2023
Genetique Reproduction and Developpement
2010
Université de Montpellier
2008
CEA Valduc
2008
Université Paul-Valéry Montpellier
2007
The transcription factor E2F plays a major role in cell cycle control mammalian cells. binding sites, which are present the promoters of variety genes required for S phase, shift from negative to positive at commitment point, crucial point G1 that precedes G1/S transition. Before activity is repressed by members pocket proteins family. This repression believed be proper growth. We have previously shown Rb, founding member family, represses E2F1 recruiting histone deacetylase HDAC1. Here, we...
The retinoblastoma susceptibility gene product, the Rb protein, is a key regulator of mammalian cell proliferation. One major targets S phase inducing E2F transcription factor. Once bound to E2F, represses expression E2F-regulated genes. Transcriptional repression by believed be crucial for proper control growth. Recently, we and others showed that through recruitment histone deacetylase. Interestingly, show here Rb-associated deacetylase complex could deacetylate polynucleosomal substrates,...
We describe here the role of histone deacetylase 3 (HDAC3) in sister chromatid cohesion and deacetylation H3 Lys 4 (H3K4) at centromere. HDAC3 knockdown induced spindle assembly checkpoint activation dissociation. The depletion Polo-like kinase 1 (Plk1) or Aurora B restored HDAC3-depleted cells. was also required for Shugoshin localization centromeres. Finally, we show that results acetylation centromeric H3K4, correlated with a loss dimethylation same position. These findings provide...
Centromeres are specialized chromosome domains that control segregation during mitosis, but little is known about the mechanisms underlying maintenance of their integrity. Centromeric ultrafine anaphase bridges physiological DNA structures thought to contain unresolved catenations between centromeres separating anaphase. BLM and PICH helicases colocalize at these promote resolution. As detectable from prometaphase onwards, we hypothesized might also be located two proteins cooperate resolve...
Abstract Histone deacetylase inhibitors (HDACI) are powerful antiproliferative drugs, and currently undergoing clinical trials as antitumor agents. It would be valuable for both cancer therapy our knowledge of basic cellular processes to understand the mechanisms by which HDACIs block cell proliferation. Most current models postulate that allow reexpression tumor suppressor genes silenced in cells. However, other mechanisms, distinct from transcription regulation, may participate HDACI...
The c-Mos proto-oncogene product plays an essential role during meiotic divisions in vertebrate eggs. In Xenopus, it is required for progression of oocyte maturation and arrest unfertilized Its degradation after fertilization to early embryogenesis. this study we investigated the mechanisms involved degradation. We present vivo evidence ubiquitin-dependent activated found that not directly dependent on anaphase-promoting factor activator Fizzy/cdc20 but requires cyclin demonstrate B/cdc2...
EWS-FLI-1 is a chimeric protein produced in most Ewing's sarcomas. It results from the fusion of N-terminal-encoding region EWS gene to C-terminal DNA-binding domain (the ETS domain) encoded by FLI-1 ets family gene. Both and proteins function as transcription factors that bind specifically sequences boxes) present promoter elements. EWS- powerful transforming protein, whereas not. In search for potential DNA binding sites these two proteins, we have tested their ability recognize serum...
Sustained spindle tension applied to sister centromeres during mitosis eventually leads uncoordinated loss of chromatid cohesion, a phenomenon known as "cohesion fatigue." We report that Aurora A-dependent phosphorylation serine 7 the centromere histone variant CENP-A (p-CENP-AS7) protects bioriented chromosomes against cohesion fatigue. Expression non-phosphorylatable version (CENP-AS7A) weakens only when are under tension, providing first evidence regulated mechanism involved in protection...
Abstract Objective To assess the genetic and epigenetic effects promoted by Bisphenol A (BPA) exposure in adolescent males from Spanish INMA-Granada birth cohort, human cells. Methods DNA methylation was analysed using MEDIP. Repeat number variation genomic evaluated, along with analysis of H3K4me3 chromatin immunoprecipitation followed high-throughput sequencing (ChIP-seq). Analyses were performed material extracted whole blood adolescents, complemented vitro assessments (HeLa) cells...
Sister chromatid cohesion is a multi-step process implemented throughout the cell cycle to ensure correct transmission of chromosomes daughter cells. While establishment and mitotic dissolution have been extensively explored, regulation cohesin loading still poorly understood. Here, we report that methyltransferase NSD3 essential for sister before mitosis entry. interacts with loader complex kollerin (NIPBL/MAU2) promotes chromatin recruitment MAU2 at exit. We also show associates in early...
We have analysed the expression of genes encoding transcription factors involved in c ‐ fos transcriptional regulation, i.e. serum response factor (SRF) and ETS‐related proteins ELK‐1 SAP‐1, a variety human cell lines. RNA was determined by Northern blot analysis, were detected on Western blots: two analyses gave essentially identical results. SRF expressed at similar levels all lines tested. In contrast, SAP‐1 varied from one line to another. Interestingly, any given line, high protein...