A5053099287- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Telomeres, Telomerase, and Senescence
- Lung Cancer Treatments and Mutations
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Cancer, Hypoxia, and Metabolism
- DNA and Nucleic Acid Chemistry
- Wnt/β-catenin signaling in development and cancer
- Cancer Research and Treatments
- Lung Cancer Research Studies
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Colorectal Cancer Treatments and Studies
- Cellular Mechanics and Interactions
- Mechanisms of cancer metastasis
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- Cell death mechanisms and regulation
- Heat shock proteins research
- Hippo pathway signaling and YAP/TAZ
- Cancer, Lipids, and Metabolism
- Gastric Cancer Management and Outcomes
- Cancer Cells and Metastasis
Japanese Foundation For Cancer Research
2016-2025
The University of Tokyo
2014-2024
Meiji Pharmaceutical University
2014-2024
Saitama Cancer Center
2023
Yokohama City University
2020
Chemotherapy Foundation
2008-2020
Koto Hospital
2012-2020
Japan Society of Chemotherapy
2017-2018
Pharmaceuticals and Medical Devices Agency
2016
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2012
Abstract Enhanced glucose and lipid metabolism is one of the most common properties malignant cells. ATP citrate lyase (ACLY) a key enzyme de novo fatty acid synthesis responsible for generating cytosolic acetyl-CoA oxaloacetate. To evaluate its role in lung cancer progression, we here analyzed ACLY expression subset human adenocarcinoma cell lines showed relationship with phosphatidyl-inositol-3 kinase–Akt pathway. The introduction constitutively active Akt into cells enhanced...
Upon treatment with various anticancer drugs, myeloid leukemia U937 cells undergo apoptosis. In this study, we found that either etoposide (VP-16) or camptothecin (CPT) activated c-Jun N-terminal kinase 1/stress-activated protein (JNK1/SAPK), transient c-jun expression, and ICE (interleukin-1beta converting enzyme)/CED-3-like proteases in cells. Phorbol ester-resistant variant, UT16 cells, displayed a decreased susceptibility to apoptosis induced by these drugs. The drugs did not cause JNK1...
Abstract Purpose: Glioma stem cells (GSC) are a critical therapeutic target of glioblastoma multiforme (GBM). Experimental Design: The effects G-quadruplex ligand, telomestatin, were evaluated using patient-derived GSCs, non-stem tumor (non-GSC), and normal fetal neural precursors in vitro vivo. molecular targets telomestatin determined by immunofluorescence situ hybridization (iFISH) cDNA microarray. data then validated vivo functional assays, as well immunohistochemistry against 90...
Tankyrase 1, a human telomeric poly(ADP-ribose) polymerase, was originally identified through its interaction with TRF1, negative regulator of telomere length. 1 ADP-ribosylates TRF1 in vitro, and overexpression induces elongation cancer cells. In addition to localization, tankyrase resides at multiple subcellular sites, suggesting additional functions for this protein. Here we identify TAB182, novel 1-binding protein 182 kDa. TAB182 displays complex pattern localization. localizes the...
In most colorectal cancers, Wnt/β-catenin signaling is activated by loss-of-function mutations in the adenomatous polyposis coli (APC) gene and plays a critical role tumorigenesis. Tankyrases poly(ADP-ribosyl)ate destabilize Axins, negative regulator of β-catenin, upregulate β-catenin signaling. Tankyrase inhibitors downregulate are expected to be promising therapeutics for cancer. However, cancer cells not always sensitive tankyrase inhibitors, predictive biomarkers drug sensitivity remain...
Telomere erosion causes cell mortality, suggesting that longer telomeres enable more divisions. In telomerase-positive human cancer cells, however, are often kept shorter than those of surrounding normal tissues. Recently, we showed telomere elongation represses innate immune genes and promotes their differentiation in vivo. This implies short contribute to malignancy, but it is unclear how such genetic repression caused by elongated telomeres. Here, report telomeric repeat-containing RNA...
Aberrant activation of Wnt/β‐catenin signaling causes tumorigenesis and promotes the proliferation colorectal cancer cells. Porcupine inhibitors, which block secretion Wnt ligands, may have only limited clinical impact for treatment cancer, because most is caused by loss‐of‐function mutations tumor suppressor adenomatous polyposis coli ( APC ) downstream ligands. Tankyrase poly( ADP ‐ribosyl)ates PAR ylates) Axin, a negative regulator β‐catenin. This post‐translational modification...
Significance During the aging process, senescent cells secrete inflammatory factors, causing various age-related pathologies. Thus, controlling senescence-associated secretory phenotype (SASP) can tremendously benefit human health. Although SASP seems to be induced by alteration of chromosomal organization, its underlying mechanism remains unclear. Here, it has been revealed that noncoding RNA (ncRNA) transcribed from pericentromeric repetitive elements impairs DNA binding CCCTC-binding...
Previous study in our laboratory demonstrated suppression of the gene for protein tyrosine phosphatase receptor-type O ( PTPRO ) primary and established rat hepatomas. The present showed methylation-mediated silencing this human lung tumors several cancer cell lines, one characteristics many tumor-suppressor genes. reduced expression correlated with methylation status its CpG island. Demethylation by deoxy-5-azacytidine treatment led to reactivation a line (A549). Overexpression A549 cells...
Lipid metabolism is often elevated in cancer cells and plays an important role their growth malignancy. Acyl-CoA synthetase (ACS), which converts long-chain fatty acids to acyl-CoA, overexpressed various types of cancer. However, the ACS remains unknown. Here, we found that enzyme activity required for cell survival. Namely, inhibitor Triacsin c induced massive apoptosis glioma while this death was completely suppressed by overexpression ACSL5, c-resistant isozyme, but not a catalytically...
Werner syndrome (WS) is a premature aging disorder characterized by chromosomal instability and cancer predisposition. Mutations in WRN are responsible for the disease cause telomere dysfunction, resulting accelerated aging. Recent studies have revealed that cells from WS patients can be successfully reprogrammed into induced pluripotent stem (iPSCs). In present study, we describe effects of long-term culture on iPSCs, which acquired maintained infinite proliferative potential self-renewal...
The canonical WNT pathway plays an important role in cancer pathogenesis. Inhibition of poly(ADP-ribose) polymerase catalytic activity the tankyrases (TNKS/TNKS2) has been reported to reduce Wnt/β-catenin signal by preventing poly ADP-ribosylation-dependent degradation AXIN, a negative regulator signaling. With goal investigating effects tankyrase and Wnt inhibition on tumor growth, we set out find small-molecule inhibitors TNKS/TNKS2 with suitable drug-like properties. Starting from 1a,...