A5086884615- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Plant Genetic and Mutation Studies
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- Cancer-related Molecular Pathways
- Cellular transport and secretion
- X-ray Spectroscopy and Fluorescence Analysis
- Developmental Biology and Gene Regulation
- Plant Molecular Biology Research
- Enzyme Structure and Function
- Electron and X-Ray Spectroscopy Techniques
Inserm
2007-2024
Centre National de la Recherche Scientifique
2008-2024
Université de Rennes
2019-2024
Institut de génétique et de développement de Rennes
2018-2024
Institut Curie
2007-2021
Université Paris Sciences et Lettres
2021
CEA Valduc
2008
Université de Montpellier
2008
Université Paul-Valéry Montpellier
2007
Clustered DNA lesions, possibly induced by ionizing radiation, constitute a trial for repair processes. Indeed, recent studies suggest that of such lesions may be compromised, potentially leading to the formation lethal double-strand breaks (DSBs). A complex multiply damaged site (MDS) composed 8-oxoguanine and 8-oxoadenine on one strand, 5-hydroxyuracil, 5-formyluracil 1 nt gap other within 17 bp was built used challenge several steps base excision (BER) pathway with human whole-cell...
We describe here the role of histone deacetylase 3 (HDAC3) in sister chromatid cohesion and deacetylation H3 Lys 4 (H3K4) at centromere. HDAC3 knockdown induced spindle assembly checkpoint activation dissociation. The depletion Polo-like kinase 1 (Plk1) or Aurora B restored HDAC3-depleted cells. was also required for Shugoshin localization centromeres. Finally, we show that results acetylation centromeric H3K4, correlated with a loss dimethylation same position. These findings provide...
Evidence has emerged that repair of clustered DNA lesions may be compromised, possibly leading to the formation double-strand breaks (DSB) and, thus, deleterious events. The first event occurring at a multiply damaged site (MDS) is major importance and will largely contribute hazardousness MDS. Here, using protein extracts from wild type or hOGG1-overexpressing Chinese hamster ovary cells, we investigated initial incision rate base damage intermediates in various complex These MDS comprise 1...
Abstract Histone deacetylase inhibitors (HDACI) are powerful antiproliferative drugs, and currently undergoing clinical trials as antitumor agents. It would be valuable for both cancer therapy our knowledge of basic cellular processes to understand the mechanisms by which HDACIs block cell proliferation. Most current models postulate that allow reexpression tumor suppressor genes silenced in cells. However, other mechanisms, distinct from transcription regulation, may participate HDACI...
Sustained spindle tension applied to sister centromeres during mitosis eventually leads uncoordinated loss of chromatid cohesion, a phenomenon known as "cohesion fatigue." We report that Aurora A-dependent phosphorylation serine 7 the centromere histone variant CENP-A (p-CENP-AS7) protects bioriented chromosomes against cohesion fatigue. Expression non-phosphorylatable version (CENP-AS7A) weakens only when are under tension, providing first evidence regulated mechanism involved in protection...
Bipolar spindle formation is essential for faithful chromosome segregation at mitosis. Because centrosomes define poles, abnormal number and structural organization of can lead to loss bipolarity genetic integrity. ASAP (aster-associated protein or MAP9) a centrosome- spindle-associated protein, the deregulation which induces severe mitotic defects. Its phosphorylation by Aurora A required assembly mitosis progression. Here, we show that localized poles Polo-like kinase 1 (Plk1) (a plays an...
Purpose: To study the effect of hydration level and plasmid packing on strand break induction in DNA by ultrasoft X-ray.Materials methods: Bluescript (pBS, tight packing) pSP189 (pSP, loose plasmids were irradiated 250, 380, 760 eV X-rays at Laboratoire pour l'Utilisation du Rayonnement Electromagnétique synchrotron facility (Orsay, France). Single double breaks (SSB DSB) quantified gel electrophoresis.Results: The number DSB per Gray Dalton pBS (5.6 ± 0.1), (6.3 0.1) (8.5 0.4)×10−12 380 eV,...
Sister chromatid cohesion is a multi-step process implemented throughout the cell cycle to ensure correct transmission of chromosomes daughter cells. While establishment and mitotic dissolution have been extensively explored, regulation cohesin loading still poorly understood. Here, we report that methyltransferase NSD3 essential for sister before mitosis entry. interacts with loader complex kollerin (NIPBL/MAU2) promotes chromatin recruitment MAU2 at exit. We also show associates in early...
Clusters of DNA damage, also called multiply damaged sites (MDS), are a signature ionizing radiation exposure. They defined as two or more lesions within one helix turns, which created by the passage single track. It has been shown that clustering damage compromises their repair. Unresolved repair may lead to formation double-strand breaks (DSB) induction mutation. We engineered three complex MDS, comprised oxidatively bases and one-nucleotide (1 nt) gap (or not), in order investigate...
Abstract Sister chromatid cohesion guarantees the correct transmission of chromosomes to daughter cells, and this multi-step process occurs throughout cell cycle. Loading core cohesin complex onto chromatin takes place during mitotic exit, establishment happens DNA replication, timely removal mitosis. While dissolution have already been explored, regulation loading is not as well understood. Here, we report that histone-lysine N-methyltransferase NSD3 an essential factor in sister...