Exposure to solar UV radiation gives rise mutations that may lead skin cancer. UVA (320-340 nm) constitutes the large majority of but is less effective than UVB (290-320 at damaging DNA. Although has been implicated in photocarcinogenesis, its contribution sunlight mutagenesis not elucidated, and DNA damage produced by remains poorly characterized. We employed HPLC-MS/MS alkaline agarose gel electrophoresis conjunction with use specific repair proteins determine distribution various classes...

In order to better understand the relative contribution of different UV components sunlight solar mutagenesis, distribution bipyrimidine photolesions, cyclobutane pyrimidine dimers (CPD), (6-4) photoproducts ((6–4)PP), and their Dewar valence photoisomers (DewarPP) was examined in Chinese hamster ovary cells irradiated with UVC, UVB, or UVA radiation simulated sunlight. The absolute amount each type photoproduct measured by using a calibrated sensitive immuno-dot-blot assay. As already...

Guanine residues in nucleic acids can be modified by treatment with N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative an vitro nonenzymatic reaction. The (ribo or deoxyribo, single double stranded) are recognized specific antibodies. They immunoprecipitated used as probes hybridization experiments detected immunochemical techniques.

UVA (320-400 nm) radiation constitutes >90% of the environmentally relevant solar UV radiation, and it has been proposed to have a role in skin cancer aging. Because popularity tanning beds prolonged periods sunbathing, potential deleterious effect emerged as source concern for public health. Although generally accepted, impact DNA damage on cytotoxic, mutagenic, carcinogenic remains unclear. In present study, we investigated sensitivity panel yeast mutants affected processing lethal...

Poly(dG-dC) . poly(dG-dC) was modified by reaction with N-acetoxy-N-acetyl-2-aminofluorene (N-AcO-AAF). Two samples 6.6% and 8.5% bases were prepared. The are randomly distributed along the polymer chain, as deduced from competition experiments between antibodies against N-2-(guanosin-8-yl)-acetylaminofluorene, poly(dG-dC), DNAs. Circular dichroism studies show that N-AcO-AAF is much more sensitive to addition of alcohol than poly(dG-dC). In about 50% (vol/vol) alcohol, both polynucleotides...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSequence context effects on 8-methoxypsoralen photobinding to defined DNA fragmentsEvelyne Sage and Ethel MoustacchiCite this: Biochemistry 1987, 26, 12, 3307–3314Publication Date (Print):June 16, 1987Publication History Published online1 May 2002Published inissue 16 June 1987https://pubs.acs.org/doi/10.1021/bi00386a010https://doi.org/10.1021/bi00386a010research-articleACS PublicationsRequest reuse permissionsArticle...

Abstract. The formation of cyclobutane pyrimidine dimers (CPD) and 8‐oxo‐7,8‐dihydro‐2′‐deoxyguanosine (8‐oxodGuo) was investigated in Chinese hamster ovary cells upon exposure to either UVC, UVB, UVA or simulated sunlight (SSL). Two cell lines were used, namely AT3‐2 UVL9, the latter being deficient nucleotide excision repair consequently UV sensitive. For all types radiation, including UVA, CPD found be predominant lesions quantitatively. At biologically relevant doses UVB SSL irradiation...

To investigate the role of nucleotide excision repair (NER) in cellular processing carcinogenic DNA photoproducts induced by defined, environmentally relevant portions solar wavelength spectrum, we have determined mutagenic specificity simulated sunlight (310-1100 nm), UVA (350-400 and UVB (290-320 as well "nonsolar" model mutagen 254-nm UVC, at adenine phosphoribosyltransferase (aprt) locus NER-deficient (ERCC1) Chinese hamster ovary (CHO) cells. The frequency distributions mutational...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSequence specificity in photoreaction of various psoralen derivatives with DNA: role biological activityVeronique Boyer, Ethel Moustacchi, and Evelyne SageCite this: Biochemistry 1988, 27, 8, 3011–3018Publication Date (Print):April 19, 1988Publication History Published online1 May 2002Published inissue 19 April 1988https://pubs.acs.org/doi/10.1021/bi00408a052https://doi.org/10.1021/bi00408a052research-articleACS PublicationsRequest reuse...

Poly (dG-dC) . poly(dG-dC) was modified by the reaction with N-acetoxy-N-acetyl-2-aminofluorene. The conformations of and poly d(G-C)AAF were studied circular dichroism under various experimental conditions. In 95% ethanol, two polynucleotides adopt A-form. 3.9 M LiCl, transition B-form-C-form is observed but not d(G-C)AAF. 1 mM phosphate buffer, behaves as a mixture B- Z-form, relative percentages depending upon amounts bases. percentage Z-form decreased addition EDTA increased Mg++....

Purpose: This work investigates whether a synergy in cell death induction exists combining atomic ions irradiation and addition of platinum salts. Such could be interest view new cancer therapy protocol based on – hadrontherapy with the radiosensitizing agents containing high-Z atoms. The experiment consists irradiating by fast cultured cells previously exposed to dichloroterpyridine Platinum (PtTC) analyzing survival colony-forming assay.Materials methods: Chinese Hamster Ovary (CHO) were...

It has been stipulated that repair of clustered DNA lesions may be compromised, possibly leading to the formation double-strand breaks (DSB) and, thus, deleterious events. Using a variety model multiply damaged sites (MDS), we investigated parameters govern DSB during processing MDS. Duplexes carrying MDS were inserted into replicative or integrative vectors, and used transform yeast Saccharomyces cerevisiae. Formation was assessed by relevant plasmid survival assay. Kinetics...

UVA has long been known for generating an oxidative stress in cells. In this paper we review the different types of DNA damage induced by UVA, i.e. strand breaks, bipyrimidine photoproducts, and oxidatively damaged bases. Emphasis is given to mechanism formation that further illustrated presentation new vitro data. Examples oxidation proteins involved metabolism are also given.

Sunlight causes lesions in DNA that if unrepaired and inaccurately replicated by polymerases yield mutations result skin cancer humans. Two enzymes involved translesion synthesis (TLS) of UV-induced photolesions are polymerase eta (Poleta) zeta (Polzeta), encoded the RAD30A REV3 genes, respectively. Previous studies have investigated TLS roles these human yeast cells irradiated with monochromatic, short wavelength UVC radiation (254 nm). However, less is known about cellular responses to...

The abbreviations used are: anti-BPDE, (+)-trans-7/3,8n-dihydroxy-9n,l0~-epoxy-7,8,9,lO-tetrahydrobenzo(a)pyrene; BPDE-N2deoxyguanosine, deoxyguanosine modified on N2 by