A5073569847- DNA Repair Mechanisms
- DNA and Nucleic Acid Chemistry
- Carcinogens and Genotoxicity Assessment
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Plant Genetic and Mutation Studies
- Advanced biosensing and bioanalysis techniques
- Synthesis and Biological Evaluation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Metal complexes synthesis and properties
- Fungal and yeast genetics research
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Musicology and Musical Analysis
- Bacteriophages and microbial interactions
- Historical, Religious, and Philosophical Studies
- Antibiotic Resistance in Bacteria
- Plant Disease Resistance and Genetics
- RNA Interference and Gene Delivery
- History of Medicine Studies
Paris Biotech Santé
2024
TH Köln - University of Applied Sciences
2022-2024
Génétique Médicale & Génomique Fonctionelle
2020-2023
Centre de Génétique Médicale de Marseille
2020-2023
Harvard University
2021-2023
Inserm
1988-2021
Aix-Marseille Université
2011-2021
Centre National de la Recherche Scientifique
2008-2019
Centre de Recherche en Cancérologie de Marseille
2009-2019
Institut Paoli-Calmettes
2014-2019
Numerous agents attack DNA, forming lesions that impair normal replication. Specialized DNA polymerases transiently replace the replicative polymerase and copy past lesions, thus generating mutations, major initiating cause of cancer. We monitored, in Escherichia coli, kinetics replication both strands molecules containing a single block either leading or lagging strand. Despite strand, lagging-strand synthesis proceeded further, implying transient uncoupling concurrent strand synthesis....
Guanine residues in nucleic acids can be modified by treatment with N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative an vitro nonenzymatic reaction. The (ribo or deoxyribo, single double stranded) are recognized specific antibodies. They immunoprecipitated used as probes hybridization experiments detected immunochemical techniques.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhysical studies on deoxyribonucleic acid after covalent binding of a carcinogenRobert Fuchs and Michel DauneCite this: Biochemistry 1972, 11, 14, 2659–2666Publication Date (Print):July 1, 1972Publication History Published online1 May 2002Published inissue 1 July 1972https://pubs.acs.org/doi/10.1021/bi00764a017https://doi.org/10.1021/bi00764a017research-articleACS PublicationsRequest reuse permissionsArticle Views70Altmetric-Citations122LEARN ABOUT...
Xeroderma pigmentosum variant (XP-V) cells are deficient in their ability to synthesize intact daughter DNA strands after UV irradiation. This deficiency results from mutations the gene encoding polymerase η, which is required for effecting translesion synthesis (TLS) past photoproducts. We have developed a simple cellular procedure identify XP-V cell strains, and subsequently analyzed 21 patients with XP-V. The 16 that we identified fall into three categories. Many of them result severe...
2-Acetylaminofluorene (AAF), a potent rat liver carcinogen that binds covalently to the C-8 position of guanine residues in DNA, is an effective frameshift mutagen. The mutations are distributed nonrandomly, most located at few specific DNA sequences (i.e., mutation hot spots). Among these spots, Nar I sequence (GGCGCC) especially susceptible induction -2 (GGCGCC----GGCC). Due nature sequence, G1G2CG3CC, three different molecular events, each involving deletion two contiguous base pairs G2C,...
Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro 7-iodo derivatives. Different ways of purification the fluorene modified samples were checked in order ot obtain a nucleic acid free from all noncovalently bound residues. The decrease melting temperature by N-AcO-AAF(DNA-AAF) carefully reinvestigated. From these experiments, we conclude that is equal to 1.15 degree C per percent bases, DNA-AAF samples. Electric dichroism measurements on...
Exposure of Escherichia coli to UV light increases expression NrdAB, the major ribonucleotide reductase leading a moderate increase in dNTP levels. The role elevated levels during translesion synthesis (TLS) across specific replication-blocking lesions was investigated. Here we show that although specialized DNA polymerase PolV is necessary for replication UV-lesions, such as cyclobutane pyrimidine dimers or pyrimidine(6-4)pyrimidone photoproduct, Pol V per se not sufficient. Indeed,...
ADVERTISEMENT RETURN TO ISSUEPerspectiveNEXTMechanisms of Frameshift Mutations: Insight from Aromatic AminesGeorge R. Hoffmann and Robert P. FuchsView Author Information Cancérogenèse et Mutagenèse Moléculaire Structurale, UPR 9003, Centre National de la Recherche Scientifique, 67400 Illkirch, FranceCite this: Chem. Res. Toxicol. 1997, 10, 4, 347–359Publication Date (Web):April 16, 1997Publication History Received30 July 1996Published online16 April 1997Published inissue 1...
Models of DNA replication in Escherichia coli involve an asymmetric polymerase complex that replicates concurrently the leading and lagging strands double-stranded DNA. The effect asymmetry on mutagenesis was tested with pairs plasmids containing unidirectional ColE1 origin a single lesion located or strand. used covalent adduct chemical carcinogen N -2-acetylaminofluorene (AAF) forms C-8 position guanine. Whether SOS induced not, mutations arose at about 20-fold higher frequency when AAF strand than