A5090927893- Calpain Protease Function and Regulation
- Neuroscience and Neuropharmacology Research
- Muscle Physiology and Disorders
- Receptor Mechanisms and Signaling
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Fatty Acid Research and Health
- Cardiomyopathy and Myosin Studies
- Signaling Pathways in Disease
- Nerve injury and regeneration
- Autism Spectrum Disorder Research
- Microtubule and mitosis dynamics
- Edible Oils Quality and Analysis
- Cardiovascular Effects of Exercise
- Cellular Mechanics and Interactions
- Hereditary Neurological Disorders
- Dialysis and Renal Disease Management
- Cardiovascular Function and Risk Factors
- Physiological and biochemical adaptations
- Nutrition and Health in Aging
- Adipose Tissue and Metabolism
- Respiratory Support and Mechanisms
- Force Microscopy Techniques and Applications
- Ion channel regulation and function
Inserm
2013-2025
Centre National de la Recherche Scientifique
2012-2025
Université de Montpellier
2013-2025
Physiologie et Médecine Expérimentale du Coeur et des Muscles
2018-2025
Institut de Génomique Fonctionnelle
2010-2022
Centre Hospitalier Universitaire de Montpellier
2021
Laboratory of Pathogens and Host Immunity
2003-2010
Royal Holloway University of London
2008
Universidad de Londres
2008
Universidad de Guanajuato
2008
Long-term controlled mechanical ventilation (CMV) in intensive care unit (ICU) induces ventilatory-induced-diaphragm-dysfunction (VIDD). The transition from CMV to assisted is a challenge that requires clinicians balance over-assistance and under-assistance. While the effects of on diaphragm are well known, we aimed assess impact under-assistance function structure piglet model with pre-existing VIDD (after long-term CMV) or without (short-term CMV). Twenty-two Large-White female piglets...
Functional interplay between ionotropic and metabotropic receptors frequently involves complex intracellular signaling cascades. The group I glutamate receptor mGlu5a co-clusters with the N-methyl-d-aspartate (NMDA) in hippocampal neurons. In this study, we report that a more direct cross-talk can exist these types of receptors. Using bioluminescence resonance energy transfer living HEK293 cells, demonstrate NMDA clustering reflects existence physical interactions. Consequently, decreased...
Scaffolding proteins interact with membrane receptors to control signaling pathways and cellular functions. However, the dynamics specific roles of interactions between different components scaffold complexes are poorly understood because dearth methods available monitor binding interactions. Using a unique combination single-cell bioluminescence resonance energy transfer imaging in living neurons electrophysiological recordings, this paper, we depict role glutamate receptor complex...
Synaptic long-term potentiation (LTP) is a key mechanism involved in learning and memory, its alteration associated with mental disorders. Shank3 major postsynaptic scaffolding protein that orchestrates dendritic spine morphogenesis, mutations of this lead to retardation autism spectrum In the present study we investigated role new Shank3-associated LTP. We identified Rho-GAP interacting CIP4 homolog 2 (Rich2) as partner by proteomic screen. Using single-cell bioluminescence resonance energy...
Calpain 1, a ubiquitous calcium‐dependent intracellular protease, was recently found in tight association with myofibrils skeletal muscle tissue [Delgado EF, Geesink GH, Marchello JA, Goll DE & Koohmaraie M (2001) J Anim Sci 79 , 2097–2107). Our immunofluorescence and immunoelectron microscopy investigations restrain the protease location at periphery of Z‐band midpoint I‐band. Furthermore, calpain 1 is to localize myofibril fractures, described as proteolysis sites, postmortem bovine...
Inter-individual variability in muscle responses to mechanical stress during exercise is poorly understood. Therefore, new cell culture scaffolds are needed gain deeper insights into the cellular mechanisms underlying influence of on human myogenic progenitor cells behavior. To this end, we propose first vitro model involving uniaxial applied aligned primary muscle-derived cells, employing a biocompatible organic-inorganic photostructurable hybrid material (OIPHM) covalently attached...
Abstract Autosomal recessive polycystic kidney disease (ARPKD) is a congenital hepatorenal fibrocystic pathology and one of the most significant childhood nephropathies leading to chronic (CKD). While damage has been well studied in this pathology, only few studies have investigated specific cardiac during ARPKD. This study aimed conduct large analysis heart dysfunction progression CKD. ARPKD rats with Pkhd1 gene mutation (IVS35‐2A>T) were monitored for CKD via echocardiography. Heart...
Limb-girdle muscular dystrophy type 2A (LGMD2A) is a recessive genetic disorder caused by mutations in the cysteine protease calpain 3 (CAPN3) that leads to selective muscle wasting. We previously showed CAPN3 deficiency associated with profound perturbation of NF-kappaB/IkappaB alpha survival pathway. In this study, consequences altered pathway were investigated using biological materials from LGMD2A patients. first show antiapoptotic factor cellular-FLICE inhibitory protein (c-FLIP), which...
By regulating actin cytoskeleton dynamics, Rho GTPases and their activators RhoGEFs are implicated in various aspects of neuronal differentiation, including dendritogenesis synaptogenesis. Purkinje cells (PCs) the cerebellum, by developing spectacular dendrites covered with spines, represent an attractive model system which to decipher molecular signaling underlying these processes. To identify novel regulators dendritic spine morphogenesis among members poorly characterized DOCK family...
Sarcopaenia, defined as a decline in both muscle mass and function, has been recognized major determinant of poor outcome haemodialysis (HD) patients. It is generally assumed that sarcopaenia driven by atrophy related to protein-energy wasting. However, dynapaenia, weakness without atrophy, characterized different disease phenotype from sarcopaenia. The aim this study was compare the characteristics prognosis sarcopaenic dynapaenic patients among prospective cohort chronic HD (CHD)...
Rationale: Prolonged mechanical ventilation is often associated with either a decrease (known atrophy) or an increase (supposed injury) in diaphragmatic thickness. Shear wave elastography noninvasive technique that measures shear modulus, surrogate of tissue stiffness and properties. Objectives: To describe changes modulus (SM) during the ICU stay relationship alterations muscle perform comprehensive ultrasound-based characterization histological force production occurring diaphragm....
Calpains have been proposed to be involved in the cytoskeletal remodeling and wasting of skeletal muscle. However, limited data are available about specific involvement each calpain early stages muscle atrophy. The aims this study were determine whether calpains 1 2 autolyzed after a short period disuse, and, if so, where myofibers products localized. In rat soleus muscle, 5 days immobilization increased particulate not soluble fraction. Conversely, was found fraction, whereas it fraction...
At glutamatergic brain synapses, scaffolding proteins regulate receptor location and function. The targeting organization of in the postsynaptic density (PSD) is poorly understood. A core protein scaffold complex, GKAP, interacts with DLC2, a associated molecular motors. In present study, we combined BRET imaging, immuno-staining electrophysiological recording to assess role GKAP-DLC2 interaction functional synapse. We found that dendritic spine stabilizes expression at PSD enhances synaptic...
Metabotropic glutamate receptors are expressed at excitatory synapses and control synaptic transmission in mammalian brain. These involved numerous patho-physiological functions. However, little is known about the molecular determinants responsible for their intracellular transport membrane targeting. Here we investigated nature of motor adaptor protein trafficking localization group I metabotropic mGlu1 postsynaptic receptor cultured hippocampal neurons. In proteomic studies, identified...
Titin is known to interact with actin thin filaments within the I‐band region of striated muscle sarcomeres. In this study, we have used a titin fragment 800 kDa (T800) purified from skeletal measure effect interaction on functional properties actin–myosin complex. MALDI‐TOF MS revealed that T800 contains entire PEVK (Pro, Glu, Val, Lys‐rich) domain. presence tropomyosin–troponin, increased sliding velocity (both average and maximum values) heavy‐meromyosin (HMM)‐coated surfaces dramatically...