A5057537791- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Microbial Natural Products and Biosynthesis
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Plant biochemistry and biosynthesis
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Polyamine Metabolism and Applications
- Autophagy in Disease and Therapy
- Metabolomics and Mass Spectrometry Studies
- Microtubule and mitosis dynamics
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Synthesis and Catalytic Reactions
- Protein Hydrolysis and Bioactive Peptides
- Adenosine and Purinergic Signaling
- Lanthanide and Transition Metal Complexes
- Enzyme Catalysis and Immobilization
- Genomics and Chromatin Dynamics
- Natural product bioactivities and synthesis
- Microbial Applications in Construction Materials
- Enzyme Structure and Function
Mount St. Mary's University
2021-2025
Johns Hopkins Medicine
2013-2021
Johns Hopkins University
2013-2021
Christopher Newport University
2015
University of Pennsylvania
2011-2015
Howard Hughes Medical Institute
2013
Polyamines are a ubiquitous class of polycationic small molecules that can influence gene expression by binding to nucleic acids. Reversible polyamine acetylation regulates acid and is required for normal cell cycle progression proliferation. Here, we report the structures Mycoplana ramosa acetylpolyamine amidohydrolase (APAH) complexed with transition state analogue hydroxamate inhibitor an inactive mutant two substrates. The structure APAH first histone deacetylase-like oligomer reveals...
Geosmin synthase from Streptomyces coelicolor (ScGS) catalyzes an unusual, metal-dependent terpenoid cyclization and fragmentation reaction sequence. Two distinct active sites are required for catalysis: the N-terminal domain ionization of farnesyl diphosphate to form germacradienol inorganic pyrophosphate (PPi), C-terminal protonation, cyclization, geosmin acetone through a retro-Prins reaction. A unique αα architecture is predicted ScGS based on amino acid sequence: each contains...
Alkylation of DNA and RNA is a potentially toxic lesion that can result in mutations even cell death. In response to alkylation damage, K63-linked polyubiquitin chains are assembled localize the Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3-Activating Signal Cointegrator 1 Complex Subunit (ASCC) repair complex damage sites nucleus. The protein ASCC2, subunit ASCC complex, selectively binds via its coupling ubiquitin conjugation ER degradation (CUE) domain. basis for...
Abstract Alkylation of DNA and RNA is a potentially toxic lesion that can result in mutations cell death. In response to alkylation damage, K63-linked polyubiquitin chains are assembled localize the ALKBH3-ASCC repair complex damage sites nucleus. The protein ASCC2, subunit ASCC complex, selectively binds using its CUE domain, type ubiquitin-binding domain typically monoubiquitin does not discriminate among different linkage types. We report here ASCC2 diubiquitin by contacting both distal...