A5103224776- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- DNA Repair Mechanisms
- Sirtuins and Resveratrol in Medicine
- Peptidase Inhibition and Analysis
- Microtubule and mitosis dynamics
- Animal Virus Infections Studies
- Biochemical effects in animals
- Viral Infections and Immunology Research
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Clostridium difficile and Clostridium perfringens research
- Calcium signaling and nucleotide metabolism
- Glycosylation and Glycoproteins Research
- PI3K/AKT/mTOR signaling in cancer
- Genetic and Kidney Cyst Diseases
- interferon and immune responses
- PARP inhibition in cancer therapy
- Renal and related cancers
- Hedgehog Signaling Pathway Studies
- Autophagy in Disease and Therapy
Johns Hopkins University
2006-2025
Johns Hopkins Medicine
2010-2025
Fujian Medical University
2022
Howard Hughes Medical Institute
2006-2013
SAGA is a transcriptional coactivator complex that conserved across eukaryotes and performs multiple functions during activation elongation. One role deubiquitination of histone H2B, this activity resides in distinct subcomplex called the deubiquitinating module (DUBm), which contains ubiquitin-specific protease Ubp8, bound to Sgf11, Sus1, Sgf73. The depends on presence all four DUBm proteins. We report here 1.90 angstrom resolution crystal structure ubiquitin aldehyde, as well 2.45...
Methylation of histone H3K4 is a hallmark actively transcribed genes that depends on mono-ubiquitination H2B (H2B-Ub). methylation in yeast catalyzed by Set1, the methyltransferase subunit COMPASS. We report here cryo-EM structure six-protein core COMPASS subcomplex, which can methylate and be stimulated H2B-Ub, bound to ubiquitinated nucleosome. Our shows spans face nucleosome, recognizing ubiquitin one nucleosome methylating H3 opposing face. As compared isolated complex, Set1 undergoes...
Sirtuins comprise a family of enzymes that catalyze the deacetylation acetyllysine side chains in reaction consumes NAD+. Although several crystal structures sirtuins bound to non-native acetyl peptides have been determined, relatively little about how discriminate among different substrates is understood. We carried out systematic structural and thermodynamic analysis single sirtuin, Sir2 homologue from Thermatoga maritima (Sir2Tm). report five forms Sir2Tm: two p53 C-terminal tail...
PTEN is a lipid phosphatase that converts phosphatidylinositol 3,4,5-phosphate (PIP3) to 4,5-phosphate (PIP2) and plays critical role in the regulation of tumor growth. subject by variety post-translational modifications, including phosphorylation on C-terminal cluster four Ser/Thr residues (380, 382, 383, 385) ubiquitylation various E3 ligases, NEDD4-1 WWP2. It has previously been shown can increase its cellular half-life. Using vitro ubiquitin transfer assays, we show WWP2 more active than...
Abstract Background Non‐small cell lung cancer (NSCLC) is one of the cancers with a high mortality rate. CircRNAs have emerged as an important regulatory factor in tumorigenesis recent years. However, detailed mechanism circular RNA cullin 2 (hsa_circ_0018189; hsa_circ_0018189) still unclear NSCLC. Methods levels hsa_circ_0018189, microRNA (miR)‐656–3p, and Solute carrier family seven member 11 (SLC7A11, xCT) were analyzed by real‐time quantitative reverse transcription‐polymerase chain...
Phosphorylation of histone H3 threonine 3 (H3T3) by Haspin recruits the chromosomal passenger complex to inner centromere and ensures proper cell cycle progression through mitosis. The mechanism which binds nucleosomes phosphorylate H3T3 is not known. We report here cryo-EM structures kinase domain bound a nucleosome. In contrast with previous histone-modifying enzymes, solely contacts nucleosomal DNA, inserting into supergroove formed apposing major grooves two DNA gyres. This unique...
ABSTRACT Methylation of histone H3K4 is a hallmark actively transcribed genes that depends on mono-ubiquitination H2B (H2B-Ub). methylation in yeast catalyzed by Set1, the methyltransferase subunit COMPASS. We report here cryo-EM structure six-protein core COMPASS subcomplex, which can methylate and be stimulated H2B-Ub, bound to ubiquitinated nucleosome. Our shows spans face nucleosome, recognizing ubiquitin one nucleosome methylating H3 opposing face. As compared isolated complex, Set1...