A5030231956- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- Endoplasmic Reticulum Stress and Disease
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Autophagy in Disease and Therapy
- Genetics and Neurodevelopmental Disorders
- Carbohydrate Chemistry and Synthesis
- Salivary Gland Disorders and Functions
- Hemoglobin structure and function
- Enzyme Production and Characterization
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- Polysaccharides and Plant Cell Walls
- Metabolism and Genetic Disorders
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Proteoglycans and glycosaminoglycans research
- Biochemical and Molecular Research
- Microbial Metabolites in Food Biotechnology
- Genetic Neurodegenerative Diseases
- interferon and immune responses
Colorado State University
2013-2025
Tufts University
2024
Collins College
2023
University at Buffalo, State University of New York
1985-2019
Bioengineering Center
2016
National Center for Genetic Engineering and Biotechnology
2016
John Wiley & Sons (United States)
2016
Hudson Institute
2016
Johns Hopkins University
1999-2012
University of Iowa
1996-2006
Ubiquitin chain complexity in cells is likely regulated by a diverse set of deubiquitinating enzymes (DUBs) with distinct ubiquitin preferences. Here we show that the polyglutamine disease protein, ataxin-3, binds and cleaves chains manner suggesting it functions as mixed linkage, chain-editing enzyme. Ataxin-3 through its amino-terminal Josephin domain carboxyl-terminal cluster interaction motifs neighboring pathogenic tract. both Lys48- or Lys63-linked yet preferentially Lys63 linkages....
Ubiquitin is a covalent signal that targets cellular proteins to the 26 S proteasome. Multiple ubiquitins can be ligated together through formation of isopeptide bonds between Lys<sup>48</sup> and Gly<sup>76</sup> successive ubiquitins. Such polyubiquitin chain constitutes highly effective proteolytic targeting signal, but its mode interaction with proteasome not well understood. Experiments address this issue have been limited by difficulties in preparing useful quantities chains uniform...
SAGA is a transcriptional coactivator complex that conserved across eukaryotes and performs multiple functions during activation elongation. One role deubiquitination of histone H2B, this activity resides in distinct subcomplex called the deubiquitinating module (DUBm), which contains ubiquitin-specific protease Ubp8, bound to Sgf11, Sus1, Sgf73. The depends on presence all four DUBm proteins. We report here 1.90 angstrom resolution crystal structure ubiquitin aldehyde, as well 2.45...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLinkage and sequence analysis of mannose-rich glycoprotein core oligosaccharides by proton nuclear magnetic resonance spectroscopyRobert E. Cohen Clinton BallouCite this: Biochemistry 1980, 19, 18, 4345–4358Publication Date (Print):September 1, 1980Publication History Published online1 May 2002Published inissue 1 September 1980https://pubs.acs.org/doi/10.1021/bi00559a031https://doi.org/10.1021/bi00559a031research-articleACS PublicationsRequest...
In at least nine inherited diseases polyglutamine expansions cause neurodegeneration associated with protein misfolding and the formation of ubiquitin-conjugated aggregates. Although expanded triggers disease, functional properties host proteins also must influence pathogenesis. Using complementary in vitro cell-based approaches we establish that disease protein, ataxin-3, is a poly-ubiquitin-binding protein. stably transfected neural cell lines, normal ataxin-3 both co-precipitate...
Invertase, extracted from broken cells of Saccharomyces cerevisiae X-2180 mm2 mannan mutant, was separated into a fraction insoluble in 75% ammonium sulfate (P75 invertase, 36% carbohydrate) and soluble (S75 53% carbohydrate). The latter reacted with antibodies specific for the alpha 1 leads to 6-linked mannose mannoprotein outer chain, whereas P75 invertase failed react this antiserum although it did serum against terminal 3-linked units that are characteristic core. A bacterial endo...
The specificity of the ubiquitin (Ub) isopeptidase in PA700 regulatory complex bovine 26 S proteasome was investigated. Disassembly poly-Ub by this enzyme is restricted to distal-end Ub substrate, i.e. farthest from site protein attachment poly-Ub-protein conjugates. determinants recognized were probed use mutant ubiquitins incorporated into Lys48-linked substrates. could not disassemble chains that contained a distal Ub(L8A,I44A). This suggested either interacts directly with Leu8 or Ile44...