A5101924082- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- Histone Deacetylase Inhibitors Research
- Genetic and phenotypic traits in livestock
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Pancreatic function and diabetes
- Renal Diseases and Glomerulopathies
- Glycosylation and Glycoproteins Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Renal Transplantation Outcomes and Treatments
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Sirtuins and Resveratrol in Medicine
- Circular RNAs in diseases
- Genetic Mapping and Diversity in Plants and Animals
Aviation General Hospital
2023-2025
National University of Singapore
2025
Minzu University of China
2019-2025
Hangzhou First People's Hospital
2019-2025
Third People's Hospital of Huzhou
2015-2025
National University Hospital
2025
Southern Medical University
2014-2024
Nanfang Hospital
2024
Shandong Provincial Hospital
2024
Shandong First Medical University
2024
Severe hyperhomocysteinemia is associated with endothelial cell injury that may contribute to an increased incidence of thromboembolic disease. In this study, homocysteine induced programmed death in human umbilical vein cells as measured by TdT-mediated dUTP nick end labeling assay, DNA ladder formation, induction caspase 3-like activity, and cleavage procaspase 3. Homocysteine-induced was specific homocysteine, not mediated oxidative stress, mimicked inducers the unfolded protein response...
The Snf-2-related CREB-binding protein activator (SRCAP) serves as a coactivator for number of transcription factors known to interact with CBP. Swr1, the closest Saccharomyces cerevisiae ortholog SRCAP, is component chromatin remodeling complex SWR-C, which catalyzes exchange histone variant H2A.Z into nucleosomes. In this report, we use combination conventional chromatography and anti-SRCAP immunoaffinity purify native human SRCAP polypeptide composition similar that show first time...
Human MOF (MYST1), a member of the MYST (Moz-Ybf2/Sas3-Sas2-Tip60) family histone acetyltransferases (HATs), is human ortholog Drosophila males absent on first (MOF) protein. catalytic subunit male-specific lethal (MSL) HAT complex, which plays key role in dosage compensation fly and responsible for large fraction H4 lysine 16 (H4K16) acetylation vivo. was recently reported to be component second designated non-specific (NSL) complex (Mendjan, S., Taipale, M., Kind, J., Holz, H., Gebhardt,...
The mammalian Tip49a and Tip49b proteins belong to an evolutionarily conserved family of AAA+ ATPases. In Saccharomyces cerevisiae, orthologs Tip49b, called Rvb1 Rvb2, respectively, are subunits two distinct ATP-dependent chromatin remodeling complexes, SWR1 INO80. We recently demonstrated that the integral a complex bearing striking similarities S. cerevisiae (Cai, Y., Jin, J., Florens, L., Swanson, K., Kusch, T., Li, B., Workman, J. Washburn, M. P., Conaway, R. C., W. (2005) Biol. Chem....
Large-scale affinity purification and mass spectrometry studies have played important roles in the assembly analysis of comprehensive protein interaction networks for lower eukaryotes. However, development such human proteins has been slowed by high cost significant technical challenges associated with systematic interactions. To address this challenge, we developed a method building local focused networks. This approach couples vector algebra statistical methods normalized spectral counting...
The multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase (HAT) complex performs critical functions in a variety of cellular processes including transcriptional activation, double strand DNA break repair, and apoptosis. We previously isolated the TRRAP/TIP60 from HeLa cells (Cai, Y., Jin, J., Tomomori-Sato, C., Sato, S., Sorokina, I., Parmely, T. Conaway, R. J. W. (2003) Biol. Chem. 278, 42733–42736). Analysis proteins present preparations led to identification several new...
8-Oxo-7,8-dihydroguanine- (8-oxoguanine-) containing nucleotides are generated in the cellular nucleotide pool by action of oxygen radicals produced during normal metabolism. We examined interconversion and metabolic fate 8-oxoguanine-containing ribonucleotides mammalian cells. (1) 8-OxoGTP can be not only direct oxidation GTP but also phosphorylation 8-oxoGDP diphosphate kinase, 8-oxoGTP thus formed serve as a substrate for RNA polymerase II to induce transcription errors. (2) MTH1 protein...
We previously identified and purified a human ATP-dependent chromatin remodeling complex with similarity to the Saccharomyces cerevisiae INO80 (Jin, J., Cai, Y., Yao, T., Gottschalk, A. Florens, L., Swanson, S. K., Gutierrez, J. Coleman, M. Workman, Mushegian, A., Washburn, P., Conaway, R. C., W. (2005) Biol. Chem. 280, 41207-41212) demonstrated that it is composed of (i) Snf2 family ATPase (hIno80) related in sequence Ino80 ATPase; (ii) seven additional evolutionarily conserved subunits...
Previous studies reported that patients with preexisting radiological interstitial lung abnormalities (ILAs) were more susceptible to developing radiation pneumonitis (RP) after thoracic therapy (TRT). The present study aimed evaluate the incidence and predictors of RP TRT in small-cell cancer (SCLC) or without ILAs.A total 95 consecutive SCLC between January 2015 December 2015, who treated intensity-modulated at Shanghai Pulmonary Hospital,Tongji University School Medicine, analyzed....
To investigate the role of long non-coding ribonucleic acid (lncRNA) nicotinamide nucleotide transhydrogenase-antisense RNA1 (NNT-AS1) in cisplatin (DDP) resistance non-small cell lung cancer (NSCLC) and its molecular mechanism.Fluorescence quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect expression levels lncRNA NNT-AS1 NSCLC lines (A549 SPCA-1) DDP-resistant (A549/DDP SPCA-1/DDP). Corresponding plasmids si-NTT-AS1 si-NC were conducted. Then, methyl thiazolyl...
Human males absent on the first (hMOF), a human ortholog of Drosophila MOF protein, is responsible for histone H4 lysine 16 (H4K16) acetylation in cells. The depletion hMOF leads to global reduction H4K16 cells, genomic instability, cell cycle defects, reduced transcription certain genes, defective DNA damage repair and early embryonic lethality. Studies have shown that abnormal gene expression involved number primary cancers. present study examined involvement clinically diagnosed ovarian...
Human MOF (males absent on the first), as a histone acetyltransferase, is responsible for H4K16 acetylation in human cells. Recent studies have shown that abnormal gene expression of hMOF involved certain primary cancers. Here, we first report involvement clinically diagnosed colorectal carcinoma (CRC) and gastric cancer. Simultaneously, correlation clinicopathological features CRC, cancer renal cell (RCC) was analyzed. The mRNA assessed 44 16 47 RCC tissue samples by quantitative PCR...
Abstract Pulmonary emphysema (PE) has been demonstrated to have a high prevalence in patients with locally advanced non-small cell lung cancer (NSCLC). A total of 153 NSCLC were enrolled this study investigate the association between PE and radiation pneumonitis (RP) after definitive thoracic therapy (TRT). The incidence RP Grade 2, 3 5 11.1%, 9.8% 0.7%, respectively. Univariate analysis revealed that age, PE, forced vital capacity (FVC), arterial partial pressure oxygen (PO 2 ) mean dose...
The Lanzhou Fat-Tail sheep (LFTS, long fat-tailed sheep) is an endangered breed in China with a fat tail compared to the traditional local varieties, Small Tail Han (STHS, thin-tailed small tail, and Tibetan (TS, short little tail. However, known regarding how deposition regulated by noncoding RNA (lncRNA). To evaluate lncRNA mRNA associated development among these breeds, high-throughput sequencing of three individuals each LFTS, STHS TS were performed analyzed this study. data from groups...
Long noncoding RNAs (lncRNA) play critical roles in cancer development. In this study, we aimed to explore the function and possible molecular mechanism of HMMR-AS1 involved lung adenocarcinoma (LUAD).Firstly, analyzed expression LUAD tissues with sequencing data from The Cancer Genome Atlas (TCGA). Next, evaluated effects on cell proliferation apoptosis, its regulation miR-138 by acting as a ceRNA. animal model was used support vitro experimental findings.HMMR-AS1 significantly upregulated...
Abstract MicroRNA‐26a‐5p (miR‐26a‐5p) has been reported to be involved in the tumorigenesis of several tumors, but its function breast cancer is still unknown. In this study, miR‐26a‐5p was found significantly downregulated both tissues and cell lines, low expression predicted a poor prognosis for patients. Overexpression could inhibit growth. Further studies revealed that overexpression protein levels Cyclin D1, CDK4, CDK6, upregulated p21, p27, p53. mechanism, targeted 3′UTR ring finger 6...