A5057023276- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Endoplasmic Reticulum Stress and Disease
- Protein Degradation and Inhibitors
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Autophagy in Disease and Therapy
- Histone Deacetylase Inhibitors Research
- Enzyme Catalysis and Immobilization
- Biochemical and Molecular Research
- Electronic and Structural Properties of Oxides
- Membrane-based Ion Separation Techniques
- DNA Repair Mechanisms
- 14-3-3 protein interactions
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Air Quality and Health Impacts
- Perovskite Materials and Applications
- Plant biochemistry and biosynthesis
University of Science and Technology of China
2025
Colorado State University
2016-2025
Chinese Academy of Sciences
2025
Huazhong Agricultural University
2025
China Pharmaceutical University
2021-2025
Tufts University
2024
Collins College
2023
Ocean University of China
2012-2022
University College London
2021
Shandong University
2021
Polycomb group (PcG) proteins repress master regulators of development and differentiation through organization chromatin structure. Mutation dysregulation PcG genes cause developmental defects cancer. form condensates in the cell nucleus, these are physical sites PcG-targeted gene silencing via formation facultative heterochromatin. However, physiochemical principles underlying remain unknown, their determination could shed light on how compact chromatin. Using fluorescence live-cell...
The mammalian Tip49a and Tip49b proteins belong to an evolutionarily conserved family of AAA+ ATPases. In Saccharomyces cerevisiae, orthologs Tip49b, called Rvb1 Rvb2, respectively, are subunits two distinct ATP-dependent chromatin remodeling complexes, SWR1 INO80. We recently demonstrated that the integral a complex bearing striking similarities S. cerevisiae (Cai, Y., Jin, J., Florens, L., Swanson, K., Kusch, T., Li, B., Workman, J. Washburn, M. P., Conaway, R. C., W. (2005) Biol. Chem....
Extracts prepared from the isolated nuclei of cultured cells have been instrumental in dissecting mechanisms by which transcription and mRNA processing occur. These extracts are able to recapitulate accurate initiation splicing vitro, has useful direct functional studies. They also serve as starting material for purification proteins that can then be reassembled studies or examined more detail biochemically. This unit describes preparation nuclear optimized production transcriptionally...
SAGA is a transcriptional coactivator complex that conserved across eukaryotes and performs multiple functions during activation elongation. One role deubiquitination of histone H2B, this activity resides in distinct subcomplex called the deubiquitinating module (DUBm), which contains ubiquitin-specific protease Ubp8, bound to Sgf11, Sus1, Sgf73. The depends on presence all four DUBm proteins. We report here 1.90 angstrom resolution crystal structure ubiquitin aldehyde, as well 2.45...
Little is known about the characteristics of particulate matter emissions from vehicles in China, although such information critical source apportionment modeling, emission inventories, and health effect studies. In this paper, we report a comprehensive characterization PM2.5 Zhujiang Tunnel Pearl River Delta region China. The chemical speciation included elemental carbon, organic inorganic ions, trace elements, compounds. factors individual species their relative distributions were obtained...
Poly [ADP-ribose] polymerase 1 (PARP-1) is a highly abundant chromatin-associated enzyme. It catalyzes the NAD(+)-dependent polymerization of long chains poly-ADP ribose (PAR) onto itself in response to DNA damage and other cues. More recently, enzymatic activity PARP-1 has also been implicated regulation gene expression. The molecular basis for functional switch from chromatin architectural protein transcription factor responder, triggered by automodification, unknown. Here, we show that...
The Polycomb PRC1 plays essential roles in development and disease pathogenesis. Targeting of to chromatin is thought be mediated by the Cbx family proteins (Cbx2/4/6/7/8) binding histone H3 with a K27me3 modification (H3K27me3). Despite this prevailing view, molecular mechanisms targeting remain poorly understood. Here, combining live-cell single-molecule tracking (SMT) genetic engineering, we reveal that H3K27me3 contributes significantly Cbx7 Cbx8 chromatin, but less Cbx2, Cbx4, Cbx6....
Nucleosomes represent mechanical and energetic barriers that RNA Polymerase II (Pol II) must overcome during transcription. A high-resolution description of the barrier topography, its modulation by epigenetic modifications, their effects on Pol nucleosome crossing dynamics, is still missing. Here, we obtain topographic transcriptional residence time) maps canonical, H2A.Z, monoubiquitinated H2B (uH2B) nucleosomes at near base-pair resolution accuracy. dynamics are complex, displaying pauses...
Post-translational modifications of histone proteins produce dynamic signals that regulate the structure and function chromatin. Mono-ubiquitination H2B in tail (at Lys-123 yeast or Lys-120 humans) is a conserved modification has been implicated regulation transcription, replication, DNA repair processes. In search for direct effectors ubH2B, we identified deubiquitinating enzyme, Usp15, through affinity purification with nonhydrolyzable ubH2B mimic. nucleus, Usp15 indirectly associates E3...
Abstract Over 80% of diffuse intrinsic pontine gliomas (DIPGs) harbor a point mutation in histone H3.3 where lysine 27 is substituted with methionine (H3.3K27M); however, how the affects kinetics and function PcG proteins remains elusive. We demonstrate that H3.3K27M prolongs residence time search Ezh2, but has no effect on its fraction bound to chromatin. In contrast, Cbx7, decreases show increasing expression Cbx7 inhibits proliferation DIPG cells time. Our results highlight directly...
Abstract Cyclodipeptide synthases (CDPSs) can catalyze the formation of two successive peptide bonds by hijacking aminoacyl-tRNAs from ribosomal machinery resulting in diketopiperazines (DKPs). Here, three CDPS-containing loci ( dmt1–3 ) are discovered genome mining and comparative analysis Streptomyces strains. Among them, CDPS DmtB1, encoded gene dmt1 locus, synthesize cyclo L -Trp- -Xaa) (with Xaa being Val, Pro, Leu, Ile, or Ala). Systematic mutagenesis experiments demonstrate importance...