A5088365197- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Enzyme-mediated dye degradation
- Protein Structure and Dynamics
- Carbohydrate Chemistry and Synthesis
- Venomous Animal Envenomation and Studies
- Trypanosoma species research and implications
- Pancreatic function and diabetes
- Ion channel regulation and function
- Enzyme Production and Characterization
- Microbial Metabolism and Applications
- Peptidase Inhibition and Analysis
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Dye analysis and toxicity
- melanin and skin pigmentation
- Hemoglobin structure and function
- Research on Leishmaniasis Studies
- Chemical Synthesis and Analysis
- Computational Drug Discovery Methods
- Monoclonal and Polyclonal Antibodies Research
- Alcohol Consumption and Health Effects
- Immune Cell Function and Interaction
- Diet, Metabolism, and Disease
Universidade de São Paulo
1995-2018
Universidade Federal de São Carlos
1995-2018
Universidad Nacional Autónoma de México
1995-2008
Instituto de Biotecnología de León
2002
Bioénergétique et Ingénierie des Protéines
2002
Queen Elizabeth II Medical Centre
2002
The University of Western Australia
2002
Universidad de la República de Uruguay
1992
Swedish University of Agricultural Sciences
1984-1990
Uppsala University
1987
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular aspects of functional differences between alcohol and sorbitol dehydrogenasesHans Eklund, Eduardo Horjales, Hans Joernvall, Carl-Ivar Braenden, Jonathan JefferyCite this: Biochemistry 1985, 24, 27, 8005–8012Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://pubs.acs.org/doi/10.1021/bi00348a025https://doi.org/10.1021/bi00348a025research-articleACS...
Conformational models of the three characterized classes mammalian liver alcohol dehydrogenase were constructed using computer graphics based on known three-dimensional structure E subunit horse enzyme (class I) and primary structures human classes. This correlates substrate-binding pockets class I subunits (alpha, beta gamma in enzyme) with those II III (pi chi, respectively) for enzymes that differ substrate specificity, inhibition pattern many other properties. The sites exhibit...
Three‐dimensional models of human alcohol dehydrogenase subunits have been constructed, based on the homologous horse enzyme, with computer graphics. All types class I (α, β, and γ) major allelic variants (β 1 /β 2 γ /γ ) studied. Residue differences between E‐type subunit enzyme any isozymes occur at 64 positions, about half which are isozyme‐specific. About two thirds substitutions surface all can be accommodated in highly conserved three‐dimensional structures. The model isozyme is most...
Abstract Mevalonate kinase (MVK) is an essential enzyme acting in early steps of sterol isoprenoids biosynthesis, such as cholesterol humans or ergosterol trypanosomatids. MVK conserved from bacteria to mammals and localizes glycosomes During the course T. cruzi characterization, we found that, addition glycosomes, this may be secreted modulate cell invasion. To evaluate role TcMVK parasite-host interactions, recombinant protein was produced anti-TcMVK antibodies were raised mice. detected...
Structural studies of proteins normally require large quantities pure material that can only be obtained through heterologous expression systems and recombinant technique. In these procedures, amounts expressed protein are often found in the insoluble fraction, making purification from soluble fraction inefficient, laborious costly. Usually, refolding is avoided due to a lack experimental assays validate correct folding compare conformational population fraction. Herein, we propose...
Model building and energy minimization procedures have been used to determine a productive substrate binding mode in liver alcohol dehydrogenase for secondary alcohols. These docking results compared some of the extensive amounts kinetic data available this enzyme. The indirect diamond lattice approach first suggested by Prelog (Prelog, V. (1964) Pure Appl. Chem. 9, 119-130) describe active site an enzyme has build direct from crystallographic model This was oriented positioned into using...
Glucosamine‐6‐phosphate deaminase (EC 3.5.99.6) is an allosteric enzyme that catalyzes the reversible conversion of D ‐glucosamine‐6‐phosphate into ‐fructose‐6‐phosphate and ammonium. Here we describe existence two mammalian glucosamine‐6‐phosphate enzymes. We present crystallographic structure one them, long human deaminase, at 1.75 Å resolution. Crystals belong to space group P2 1 2 a whole hexamer in asymmetric unit. The active‐site lid (residues 162–182) presented significant structural...
Glossoscolex paulistus is a free-living earthworm encountered in south-east Brazil. Its oxygen transport requirements are undertaken by giant extracellular haemoglobin, or erythrocruorin (HbGp), which has an approximate molecular mass of 3.6 MDa and, analogy with its homologue from Lumbricus terrestris (HbLt), believed to be composed total 180 polypeptide chains. In the present work full particle cyanomet state was purified and crystallized using sodium citrate PEG8000 as precipitant. The...
A new crystallographic structure of the free active-site R conformer allosteric enzyme glucosamine-6-phosphate deaminase from Escherichia coli, coupled with previously reported structures T and conformers, generates a detailed description heterotropic transition in which structural flexibility plays central role. The conformer's external zone [Horjales et al. (1999), Structure, 7, 527–536] presents higher B values than conformers. ligand-free (T conformer) undergoes an to upon binding...
The antibody BCF2 generated against the mammal-specific toxin Cn2 of scorpion Centruroides noxius Hoffmann neutralizes effect both and venom. We cloned sequenced genes coding for Fv fragment BCF2. A three-dimensional (3D) model was using a knowledge-based approach. Furthermore, 3D complex Cn2–BCF2 built nuclear magnetic resonance (NMR) structure experimental results on putative epitope region around N C termini. initial conformations were submitted to new refinement procedure rigid-body...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAsymmetric allosteric activation of Escherichia coli glucosamine-6-phosphate deaminase produced by replacements Tyr 121Myriam M. Altamirano, Jacqueline A. Plumbridge, Eduardo Horjales, and Mario L. CalcagnoCite this: Biochemistry 1995, 34, 18, 6074–6082Publication Date (Print):May 9, 1995Publication History Published online1 May 2002Published inissue 9...