A5037661087- Neuropeptides and Animal Physiology
- Stress Responses and Cortisol
- Hypothalamic control of reproductive hormones
- Thyroid Disorders and Treatments
- Regulation of Appetite and Obesity
- Growth Hormone and Insulin-like Growth Factors
- Neuroendocrine regulation and behavior
- Adipose Tissue and Metabolism
- Peptidase Inhibition and Analysis
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Biochemical effects in animals
- Birth, Development, and Health
- Receptor Mechanisms and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Reproductive System and Pregnancy
- Biochemical Analysis and Sensing Techniques
- Neuroendocrine Tumor Research Advances
- Signaling Pathways in Disease
- Nerve injury and regeneration
- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- Nicotinic Acetylcholine Receptors Study
- Antimicrobial Peptides and Activities
- Hormonal Regulation and Hypertension
Universidad Nacional Autónoma de México
2014-2025
Universidad Autónoma del Estado de Morelos
2017
Instituto Nacional de Psiquiatría
2000
Sorbonne Université
1991-1993
Centre National de la Recherche Scientifique
1991
Centro de Ingeniería Genética y Biotecnología
1988
University of Texas Health Science Center at Dallas
1979-1981
The University of Texas Health Science Center at Houston
1981
University of Pittsburgh
1980
Centre Paul Broca
1979
Glucocorticoids and corticotropin-releasing hormone (CRH) are key regulators of stress responses. Different types activate the CRH system; in hypothalamus, expression release increased by physical or psychological stressors while amygdala, preferentially stress. Learning memory processes modulated glucocorticoids at different levels. To characterize kind provoked a hippocampal-dependent task such as spatial learning, we compared profile glucocorticoid receptor (GR), pro-CRH CRH-R1 mRNAs...
The in vitro release of TRH from hypothalamic fragments or purified nerve endings (synaptosomes) has been evaluated after incubation for 10 min the presence various concentrations K+ neurotransmitters. Release hormone but not synaptosomes was enhanced 56 mM a Ca++-dependent manner. Neurotransmitter effects were thus tested on fragments. Addition histamine (10-7-10-5 M) induced significant increase over basal TRH. A comparable effect obtained with dimaprit (10-5 M), highly specific agonist H1...
Thyrotropin releasing hormone (TRH) is released from the median eminence in response to neural stimuli evoked by different physiologic conditions (i.e. cold stress or suckling). The paraventricular nucleus (PVN) synthesizes pro-TRH and responds negative thyroid feedback. With aim of determining if TRH biosynthesis regulated coordination with its release, we quantified mRNA levels PVN preoptic area-anterior hypothalamus (POA-AH) rats sacrificed at times during (0.5, 1,2 6 h) suckling (15, 30...
Thyrotropin-releasing hormone (TRH) is released from the median eminence upon neural stimulation such as cold or suckling exposure. Concomitant with cold- suckling-induced release of TRH a rapid and transient increase in expression proTRH mRNA paraventricular nucleus (PVN) hypothalamus. We employed two strategies to determine whether neurons responding exposure are different those suckling. First, we attempted identify marker cellular activation PVN. Cold induced <i>c-fos</i>...
The hypothalamic-pituitary thyroid (HPT) axis modulates energy homeostasis. Its activity decreases in conditions of negative balance but the effects chronic exercise on are controversial and unknown at hypothalamic level. Wistar male rats were exposed for up to 14 days voluntary wheel running (WR), or pair-feeding (PF; 18% food restriction), repeated restraint (RR), a mild stressor. WR RR diminished intake; body weight gain decreased 3 experimental groups, WAT mass serum leptin more...
Fasting down-regulates the hypothalamus-pituitary-thyroid (HPT) axis activity through a reduction of TRH synthesis in neurons parvocellular paraventricular nucleus hypothalamus (PVN). These project to median eminence (ME), where terminals are close cytoplasmic extensions β2 tanycytes. Tanycytes express pyroglutamyl peptidase II (PPII), TRH-degrading ectoenzyme that controls amount reaches anterior pituitary. We tested hypothesis regulation ME PPII is another mechanism by which fasting...
Hypothalamic-pituitary-thyroid (HPT) axis activity is important for energy homeostasis, and modified by stress. Maternal separation (MS) alters the stress response predisposes to metabolic disturbances in adult. We therefore studied effect of MS on adult HPT activity. Wistar male female pups were separated from their mothers 3 h/d during postnatal day (PND)2–PND21 (MS), or left nonhandled (NH). Open field elevated plus maze tests revealed increased locomotion males anxiety-like behavior...
Based on the type-I cannabinoid receptor (CB1) content of hypophysiotropic axons and involvement tanycytes in regulation hypothalamic-pituitary-thyroid (HPT) axis, we hypothesized that endocannabinoids are involved tanycyte-induced TRH release median eminence (ME). We demonstrated CB1-immunoreactive were associated to DAGLα-immunoreactive tanycyte processes external zone ME showed tonically inhibit this tissue. glutamate depolarizes tanycytes, increases their intracellular Ca2+ level 2-AG...
The amplitude of the phasic output thyrotropin-releasing hormone (TRH) into hypothalamus-pituitary portal capillaries is likely controlled by TRH-degrading ectoenzyme (TRH-DE) expressed on surface median eminence (ME) β2-tanycytes. To extend this hypothesis, we performed experiments in adult rodents reared standard conditions. TRH-DE was close to putative sites TRH release male rat external layer ME. In global Trhde knock out mice, basal hypothalamus-pituitary-thyroid (HPT) axis parameters...
The amplitude of the phasic output thyrotropin-releasing hormone (TRH) into hypothalamus-pituitary portal capillaries is likely controlled by TRH-degrading ectoenzyme (TRH-DE) expressed on surface median eminence (ME) β2-tanycytes. To extend this hypothesis, we performed experiments adult rodents reared in standard conditions. TRH-DE was close to putative sites TRH release male rat external layer ME. In global Trhde knockout mice, basal hypothalamus-pituitary-thyroid (HPT) axis parameters...
The biosynthesis of thyrotropin-releasing hormone (TRH) in the hypothalamic paraventricular nucleus (PVN) is subject to neural and hormonal regulations. To identify some potential effectors this modulation, we incubated dispersed cells with dexamethasone for short periods time (1–3 h) studied interaction protein kinase C (PKC) PKA signaling pathways. TRH mRNA relative changes were determined by RT-PCR technique. One hour incubation 10<sup>–10</sup>–10<sup>–4</sup>...
Proteolytic processing of somatostatin precursor produces several peptides including somatostatin-14 (S-14), somatostatin-28 (S-28), and (1-12) (S-28(1-12)). The subcellular sites at which these cleavages occur were identified by quantitative evaluation products in enriched fractions the biosynthetic secretory apparatus rat cortical or hypothalamic cells. Each major cellular compartments was obtained discontinuous gradient centrifugation characterized both specific enzyme markers electron...