A5013139407- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Lipid Membrane Structure and Behavior
- Graphene and Nanomaterials Applications
- Drug Solubulity and Delivery Systems
- Cell Image Analysis Techniques
- Advancements in Transdermal Drug Delivery
- Advanced Drug Delivery Systems
- Crystallization and Solubility Studies
- Heavy Metal Exposure and Toxicity
- Nanoplatforms for cancer theranostics
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Microplastics and Plastic Pollution
- Toxin Mechanisms and Immunotoxins
- Pesticide and Herbicide Environmental Studies
- 3D Printing in Biomedical Research
- Polymer-Based Agricultural Enhancements
- Healthcare and Environmental Waste Management
- Carbon and Quantum Dots Applications
- Chemistry Education and Research
- Anesthesia and Neurotoxicity Research
- Food Chemistry and Fat Analysis
Universidade Estadual de Campinas (UNICAMP)
2016-2025
Biolog (United States)
2025
National University of Lomas de Zamora
2018
University of Groningen
2010-2015
University Medical Center Groningen
2010-2015
Antibiotic-induced dysbiosis is a key factor predisposing intestinal infection by Clostridium difficile. Here, we show that interventions restore butyrate levels mitigate clinical and pathological features of C. difficile-induced colitis. Butyrate has no effect on difficile colonization or toxin production. However, it attenuates inflammation improves barrier function in infected mice, as shown reduced epithelial permeability bacterial translocation, effects associated with the increased...
Poly(epsilon-caprolactone) (PCL) nanocapsules have been recently developed as a modified release system for atrazine, an herbicide that can harmful effects in the environment. Here, post-emergence herbicidal activity of PCL containing atrazine was evaluated using mustard (Brassica juncea) target plant species model. Characterization atrazine-loaded by nanoparticle tracking analysis indicated concentration 7.5 x 1012 particles mL-1 and average size distribution 240.7 nm. The treatment plants...
Atrazine is one of the most used herbicides and has been associated with persistent surface groundwater contamination, novel formulations derived from nanotechnology can be a potential solution. We poly(ε-caprolactone) nanoencapsulation atrazine (NC+ATZ) to develop highly effective herbicidal formulation. Detailed structural study interaction between formulation Brassica juncea plants was carried out evaluation foliar uptake nanoatrazine alterations induced in leaves. Following postemergent...
The blood–brain barrier (BBB) is a complex physical and functional protecting the central nervous system from chemical insults. Nevertheless, it also constitutes against therapeutics for treating neurological disorders. In this context, nanomaterial-based therapy provides potential alternative overcoming problem. Graphene family has attracted significant interest in nanomedicine because their unique physicochemical properties make them amenable to applications drug/gene delivery neural...
Polyethylene glycol (PEG) coating has been frequently used to improve the pharmacokinetic behavior of nanoparticles. Studies that contribute better unravel effects PEGylation on toxicity nanoparticle formulation are therefore highly relevant. In present study, reduced graphene oxide (rGO) was functionalized with PEG, and its key components blood-brain barrier, such as astrocytes endothelial cells, were analyzed in culture an vivo rat model. The vitro studies demonstrated...
We have previously demonstrated that reduced graphene oxide (rGO) administered intravenously in rats was detected inside the hippocampus after downregulation of tight and adherens junction proteins blood-brain barrier. While down-regulators junctional could be useful tools for drug delivery through paracellular pathway, concerns over toxicity must investigated before clinical application. Herein, our purpose to trace whether rGO triggered toxic alterations this brain region target organs...
Poly(epsilon-caprolactone) (PCL) nanocapsules have been used as a carrier system for the herbicide atrazine, which is commonly applied to maize. We demonstrated previously that these atrazine containing polymeric were ten-fold more effective in control of mustard plants (a target species), compared commercial formulation. Since can adverse effects on non-target crops, here we analyzed effect encapsulated growth, physiological and oxidative stress parameters soil-grown maize (Zea mays L.)....
Abstract Image‐based profiling quantitatively assesses the effects of perturbations on cells by capturing a breadth changes via microscopy. Here, we provide two complementary protocols to help explore and interpret data from image‐based experiments. In first protocol, examine similarity among perturbed cell samples using compounds that cluster their mechanisms action. The protocol includes steps feature‐driving differences between visualize correlations features treatments create...
A new nanohydrogel drug delivery platform based on Laponite nanodiscs, polyacrylate, and sodium phosphate salts is described. The hybrid tailored to obtain soft flexible nanohydrogels with G′ around 3 kPa, which has been proposed as the ideal stiffness for applications. In vitro studies demonstrate that are biocompatible, biodegradable, nonswellable, pH-responsive, noncytotoxic able deliver antineoplastic drugs into cancer cells. IC50 of containing cisplatin, 4-fluorouracil, cyclophosphamide...
Microfluidics offers unique characteristics to control the mixing of liquids under laminar flow. Its use for assembly lipoplexes represents an attractive alternative translation gene delivery studies into clinical trials on a sufficient throughput scale. Here, it was shown that microfluidic pDNA/cationic liposome (CL) allows formation nanocarriers with enhanced transfection efficiencies compared conventional bulk-mixing (BM) process high pDNA loading conditions. Lipoplexes generated by...
This study aimed to explore the antibacterial and anticancer potential of biosynthesized silver nanoparticles how biosynthesis AgNP@Bio determines their biological roles.
Abstract Objectives To investigate the molecular interaction between β-cyclodextrin (βCD) or hydroxypropyl-β-cyclodextrin (HPβCD) and riboflavin (RF), to test anticancer potential of these formulations. Methods The physicochemical characterization association RF CDs was performed by UV-vis absorption, fluorescence, differential scanning calorimetry NMR techniques. Molecular dynamics simulation used shed light on mechanism CDs. Additionally, in-vitro cell culture tests were evaluate...
Pancreatic cancer ranks fourth among cancer-related causes of death in North America. Minimal progress has been made the diagnosis and treatment patients with late-stage tumors. Moreover, pancreatic aggressiveness is closely related to high levels pro-survival mediators, which can ultimately lead rapid disease progression, resistance metastasis. The main goal this study was define mechanisms by calix[6]arene, but not other calixarenes, efficiently decreases a drug resistant human pancreas...
pH-sensitive liposomes are interesting carriers for drug-delivery, undertaking rapid bilayer destabilization in response to pH changes, allied tumor accumulation, a desirable behavior the treatment of cancer cells. Previously, we have shown that accumulate tissues mice, which an acidic environment accelerates drug delivery. Ultimately, these formulations can be internalized by cells and take endosome-lysosomal route. However, mechanism doxorubicin release intracellular traffic remains...
CellProfiler is a widely used software for creating reproducible, reusable image analysis workflows without needing to code. In addition the >90 modules that make up main program, has plugins system allows creation of new which integrate with other Python tools or are packaged in containers. The CellProfiler-plugins repository contains number these modules, especially experimental and/or dependency-heavy. Here, we present an upgraded repository, example accessing containerised tools,...
Nanotechnology applications in biomedicine have increased recent decades, primarily as therapeutic agents, drugs, and gene delivery systems. Among the nanoparticles used medicine, we highlight cationic solid lipid (SLN). Given their nontoxic properties, much research has focused on beneficial effects of SLN for drug or system. However, little attention been paid to adverse impacts cellular environment, particularly influence intracellular signaling pathways. In this work, investigate...