A5068969840- Ion channel regulation and function
- Graphene and Nanomaterials Applications
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Ion Transport and Channel Regulation
- Erythrocyte Function and Pathophysiology
- Barrier Structure and Function Studies
- Cancer-related molecular mechanisms research
- Caveolin-1 and cellular processes
- Venomous Animal Envenomation and Studies
- Nanoparticle-Based Drug Delivery
- RNA modifications and cancer
- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Carbon and Quantum Dots Applications
- Axon Guidance and Neuronal Signaling
- Medicinal Plants and Neuroprotection
- Diet and metabolism studies
- Healthcare and Venom Research
- Moringa oleifera research and applications
- Bioactive natural compounds
- Ginseng Biological Effects and Applications
Josep Carreras Leukaemia Research Institute
2021-2025
National Council for Scientific and Technological Development
2021-2025
Institut d'Investigació Biomédica de Bellvitge
2017-2021
Universidade Estadual de Campinas (UNICAMP)
2012-2020
Duran i Reynals Hospital
2017
Universidade Presbiteriana Mackenzie
2015
The blood–brain barrier (BBB) is a complex physical and functional protecting the central nervous system from chemical insults. Nevertheless, it also constitutes against therapeutics for treating neurological disorders. In this context, nanomaterial-based therapy provides potential alternative overcoming problem. Graphene family has attracted significant interest in nanomedicine because their unique physicochemical properties make them amenable to applications drug/gene delivery neural...
Polyethylene glycol (PEG) coating has been frequently used to improve the pharmacokinetic behavior of nanoparticles. Studies that contribute better unravel effects PEGylation on toxicity nanoparticle formulation are therefore highly relevant. In present study, reduced graphene oxide (rGO) was functionalized with PEG, and its key components blood-brain barrier, such as astrocytes endothelial cells, were analyzed in culture an vivo rat model. The vitro studies demonstrated...
We have previously demonstrated that reduced graphene oxide (rGO) administered intravenously in rats was detected inside the hippocampus after downregulation of tight and adherens junction proteins blood-brain barrier. While down-regulators junctional could be useful tools for drug delivery through paracellular pathway, concerns over toxicity must investigated before clinical application. Herein, our purpose to trace whether rGO triggered toxic alterations this brain region target organs...
Abstract The hyperphosphorylation of microtubule‐associated protein tau (tau) in the hippocampus can be caused by central and peripheral insulin resistance these alterations are related to development tauopathies, such as Alzheimer's disease. In this study, we used a high‐fat diet induce obesity adult Swiss mice checked whether supplementation with Myrciaria jaboticaba berry peel for 10 weeks could improve sensitivity, learning/memory performance, prevent phosphorylation hippocampus....
Natural compounds could be a complementary alternative to inflammatory bowel disease (IBD) management. This study determined the effects of an aqueous extract Myrciaria jaboticaba peel (EJP) (50 g L-1) on 2,4,6-trinitrobenzenesulfonic acid-induced colitis. Wistar rats were randomized into five groups: HC-healthy control, CC-colitis DC-drug SJ-short-term treatment with EJP, and LJ-long-term EJP. The EJP treatments reduced body weight loss, stool consistency score, spleen enlargement. Gut...
In this study, we supplemented a high-fat diet (HF) with 5% powder of freeze-dried Syzygium malaccense fruit (HS) and investigated proofs peripheral insulin resistance, hippocampal AKT–GSK3-β–tau activation, learning/memory performance, brain frontal lobe oxidative stress. The intake HS did not prevent weight gain but promoted sensitivity, improved AKT signaling in the hippocampus, which prevented activation GSK3-β lowered tau phosphorylation induced HF. results from Morris water maze...
Rett syndrome (RTT) is a severe neurodevelopmental disorder primarily caused by loss-of-function mutations in the MECP2 gene, resulting diverse cellular dysfunctions. Here, we investigated role of long noncoding RNA (lncRNA) NEAT1 context MeCP2 deficiency using human neural cells and RTT patient samples. Through single-cell sequencing molecular analyses, found that markedly downregulated knockout (KO) at various stages differentiation. downregulation correlated with aberrant activation mTOR...
Two astrocyte markers, the glial water channel aquaporin-4 (AQP4) and fibrillary acidic protein (GFAP), have been implicated in several physiological pathological conditions central nervous system (CNS) as well blood-brain barrier breakdown (BBBb). By color segmentation immunoreactivity of both proteins, we demonstrate that expression AQP4 GFAP was increased cerebellum neonate (14-day-old, P14) adult (8-week-old) rats administered Phoneutria nigriventer spider venom (PNV) known to cause...
Spider venoms contain neurotoxic peptides aimed at paralyzing prey or for defense against predators; that is why they represent valuable tools studies in neuroscience field. The present study identifying the process of internalization occurs during increased trafficking vesicles caused by Phoneutria nigriventer spider venom (PNV)-induced blood–brain barrier (BBB) breakdown. Herein, we found caveolin-1α up-regulated cerebellar capillaries and Purkinje neurons PNV-administered P14 (neonate) 8-...
This study characterizes the distribution and quantifies expression of tyrosine kinase receptor for vascular endothelial growth factor (VEGF), Flt-1, in rat hippocampus following intra-peritoneal injection Phoneutria nigriventer venom (PNV). Post-natal day 14 (P14) 8-10 weeks (adult) old rats were used analyses done at 1, 2, 5 24 h after exposure compared with saline-injected counterparts. PNV-injected animals showed hippocampal venules perivascular edema indicating blood-brain barrier (BBB)...
Apart from its angiogenic and vascular permeation activity, the endothelial growth factor (VEGF) has been also reported as a potent neuronal protector. Newborn rats with low VEGF levels develop neuron degeneration, while high induce protective mechanisms in several neuropathological conditions. Phoneutria nigriventer spider venom (PNV) disrupts blood-brain barrier (BBB) causes neuroinflammation central neurons along excitotoxic signals humans. All these changes are transient. Herein, we...
Phoneutria nigriventer spider accidental envenomation provokes neurotoxic manifestations, which when critical, results in epileptic-like episodes. In rats, P. venom (PNV) causes blood-brain barrier breakdown (BBBb). The PNV-induced excitotoxicity from disturbances on Na(+), K(+) and Ca(2+) channels glutamate handling. vascular endothelial growth factor (VEGF), beyond its angiogenic effect, also, interferes synaptic physiology by affecting the same ion protects neurons excitotoxicity....
Numerous studies have shown that the venom of Phoneutria nigriventer (PNV) armed-spider causes excitotoxic signals and blood-brain barrier breakdown (BBBb) in rats. Nitric oxide (NO) is a signaling molecule which has role endothelium homeostasis vascular health. The present study investigated relevance endothelial NO synthase (eNOS) uncoupling to clinical neurotoxic evolution induced by PNV. eNOS immunoblotting cerebellum lysates processed through low-temperature SDS-PAGE revealed...
Noncoding RNAs play regulatory roles in physiopathology, but their involvement neurodevelopmental diseases is poorly understood. Rett syndrome a severe, progressive disorder linked to loss-of-function mutations of the
An important transcellular transport mechanism in the blood-brain barrier (BBB) involves caveolae, which are specialized delta-shaped domains of endothelial plasma membrane that rich cholesterol, glycosphingolipids and scaffolding protein Caveolina-1 (Cav-1). In this work, we investigated whether increase endocytosis transendothelial vesicular trafficking rat cerebellum after breakdown (BBBb) induced by Phoneutria nigriventer spider venom (PNV) was mediated caveolae. The expression Cav-1,...
Transcribed ultraconserved regions (T-UCRs) are noncoding RNAs derived from DNA sequences that entirely conserved across species. Their expression is altered in many tumor types, and, although a role for T-UCRs as regulators of gene has been proposed, their functions remain largely unknown. Herein, we describe the epigenetic silencing uc.160+ T-UCR gliomas and mechanistically define novel RNA-RNA regulatory network which modulates biogenesis several members miR-376 cluster. This includes...
An immortalized neural cell line derived from the human ventral mesencephalon, called ReNCell, and its MeCP2 knock out were used. With it, we characterized chromatin compositional transitions undergone during differentiation, with special emphasis on linker histones. While WT cells displayed development of dendrites axons KO did not, despite undergoing differentiation as monitored by NeuN. ReNCell expressed minimal amounts histone H1.0 their complement consisted mainly H1.2, H1.4 H1.5. The...
Phoneutria nigriventer spider venom (PNV) contains ion channels-acting neuropeptides that in rat induces transitory blood-brain barrier breakdown (BBBb) hippocampus parallel with VEGF upregulation. We investigated whether has a neuroprotective role by inhibiting its binding to receptor Flk-1 itraconazole (ITZ). FT-IR spectroscopy examined the biochemical status of and evaluated BBBb rats administered PNV or ITZ/PNV at periods greatest toxicity (1-2h), recovery (5h) visual absence symptoms...
We have previously demonstrated that Phoneutria nigriventer venom (PNV) causes blood–brain barrier (BBB) breakdown, swelling of astrocytes end-feet and fluid permeation into brain interstitium in rats. Caveolae water channels respond to BBB alterations by co-participation shear stress response edema formation/resolution. Herein, we showed post-natal developmental-related changes two BBB-associated transporter proteins: the endothelial caveolin-1 (Cav-1), major scaffolding protein from...
This study was undertaken to elucidate why VEGF/VEGFR-2 is elevated in the hippocampus of rats injected with Phoneutria nigriventer spider venom (PNV). PNV delays Na+ channels inactivation; blocks Ca2+ and K+ channels, increases glutamate release, causes blood-brain breakdown (BBBb), brain edema severe excitotoxicity. Analytical FT-IR spectroscopy showed profound alteration molecular biochemical state, evidences for VEGFR-2 (KDR/Flk-1) signaling mediation. By blocking binding via...