A5088622218- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Viral Infections and Immunology Research
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Biochemical and Molecular Research
- Hepatitis Viruses Studies and Epidemiology
- Silicon and Solar Cell Technologies
- Cardiomyopathy and Myosin Studies
- Insect symbiosis and bacterial influences
- Industrial Vision Systems and Defect Detection
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- RNA Research and Splicing
- T-cell and B-cell Immunology
- Synthesis and Biological Evaluation
- Viral Infectious Diseases and Gene Expression in Insects
- Leprosy Research and Treatment
- RNA and protein synthesis mechanisms
- Viral Infections and Outbreaks Research
- Cystic Fibrosis Research Advances
- Liquid Crystal Research Advancements
- Congenital Ear and Nasal Anomalies
- Genomics and Phylogenetic Studies
Fundação Oswaldo Cruz
2010-2023
Fundação Carlos Chagas
2011-2023
Universidade Federal do Paraná
2000-2018
University of Bayreuth
2016
ETH Zurich
2016
Derma Consult (Germany)
2012
Institute of Inorganic Chemistry of the Slovak Academy of Sciences
2012
Instituto de Biologia Molecular do Paraná
2003-2011
Universidade Federal do Rio de Janeiro
2007
Pontifícia Universidade Católica do Paraná
2007
Spiders of the Loxosceles genus are cosmopolitan, and their venom components possess remarkable biological properties associated with ability to act upon different molecules receptors. Accidents intermedia specimens recognized as a public health problem in south Brazil. To describe transcriptional profile L. gland, we generated wide cDNA library, its transcripts were functionally structurally analyzed. After initial analyses, 1843 expressed sequence tags (ESTs) produced readable sequences...
The protozoan parasite Trypanosoma cruzi displays similarities to fungi in terms of its sterol lipid biosynthesis, as ergosterol and other 24-alkylated sterols are principal endogenous sterols. pathway is thus a potential drug target for the treatment Chagas disease. We describe here comparative study growth inhibition, ultrastructural physiological changes leading death T. cells following with biosynthesis inhibitors (SBIs) ketoconazole lovastatin. first calculated concentration inhibiting...
Trypanosoma cruzi is the etiologic agent of Chagas disease, which estimated to affect over eight million people around world. has a complex life cycle, involving insect and mammalian hosts four distinct developmental stages: epimastigotes, metacyclic trypomastigotes, amastigotes, bloodstream trypomastigotes. Metacyclogenesis process by T. epimastigotes differentiate into trypomastigotes acquire infectivity, involves differential gene expression associated with acquisition virulence. In ,...
The study of the HLA variability Native American populations revealed several alleles specific to one or more Latin indigenous populations. analysis Amerindian groups distributed all over continent might inform about area origin and dispersal these shed light on evolution this remarkable polymorphism. Moreover, haplotypes are excellent markers understand genetic relationships between For reasons, we characterized class II polymorphism in seven South compared results with those previously...
Protein phosphorylation is a reversible post-translational modification essential for the regulation of several signal transduction pathways and biological processes in living cell. Therefore, identification protein sites crucial to understand cell signaling control at molecular level. Based on mass spectrometry, recent studies have reported large-scale mapping various eukaryotes prokaryotes. However, little known about impact protozoan parasites. To depth characterize phosphoproteome...
ABSTRACT Chagas' disease, caused by the hemoflagellate protozoan Trypanosoma cruzi , affects millions of people in South and Central America. Chronic chagasic cardiomyopathy, most devastating manifestation this occurs approximately one-third infected individuals. Events associated with parasite's tropism for invasion cardiomyocytes have been focus intense investigation recent years. In present study, we use murine microarrays to investigate cellular response primary T. trypomastigotes. These...
Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit single invertebrate during their entire life cycle and exhibit special characteristics that group them particular phylogenetic cluster Trypanosomatidae family, thus classified as monoxenics. In effort to better understand such symbiotic association, we used DNA...
A route to fully miscible polyethylene (PE) nanocomposites has been established based on polymer-brush-coated nanoparticles. These nanoparticles can be mixed with PE at any ratio, homogeneous dispersion, and without aggregation. This allowed a first systematic study of the thermomechanical properties interference from aggregation effects. We observe that storage modulus in semicrystalline state softening temperature increase significantly increasing nanoparticle content, whereas melt...
SUMMARY Trypanosoma cruzi , the etiological agent of Chagas disease, is ingested by triatomines during their bloodmeal on an infected mammal. Aiming to investigate development and differentiation T. inside intestinal tract Rhodnius prolixus at beginning infection we fed insects with cultured epimastigotes blood trypomastigotes from mice determine amount recovered parasites after ingestion. Approximately 20% was found in insect anterior midgut (AM) 3 h feeding. Interestingly, a significant...
A novel nanoimprint lithography technique based on the photofluidization effect of azobenzene materials is presented. The tunable process allows for imprinting under ambient conditions without crosslinking reactions, so that shrinkage resist avoided. Patterning surfaces in regime from micrometers down to 100 nm demonstrated. As a service our authors and readers, this journal provides supporting information supplied by authors. Such are peer reviewed may be re-organized online delivery, but...
Trypanosoma cruzi, the etiologic agent of Chagas disease, has a complex life cycle in which four distinct developmental forms alternate between insect vector and mammalian host. It is assumed that replicating epimastigotes present gut are not infective to host, paradigm corroborated by widely acknowledged fact only this stage susceptible complement system. In work, we establish T. cruzi vitro vivo epimastigogenesis model analyze biological aspects recently differentiated (rdEpi). We show...
The annually produced quantity of solar modules has steadily increased over the past decades. Rising production speeds and associated high throughput wafers, cells, will make an automatized quality inspection mandatory. In case visual optical inspection, control by using machine vision is already possible. To localize cracks in luminescence imaging used, where several approaches for exist, but a standard solution algorithm not yet available. This is, particular, true multicrystalline grainy...
Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence codes a 21kDa protein selected. After cloning this sequence, the results it ubiquitous by extracellular amastigotes. The recombinant form (P21-His(6)) adhered to HeLa cells dose-dependent manner. Pretreatment host with P21-His(6) inhibited cell invasion amastigotes from G CL strains. On other...
Capsid assembly is the final event of virus replication, and its understanding pivotal for design empty capsid-based recombinant vaccines drug delivery systems. Although capsid structure several members picornavirus family has been elucidated, little known about structural elements governing process that tightly associated with proteolytic processing viral polyprotein. Among picornaviruses, hepatitis A (HAV) unique in it contains VP1-2A as a component small protein VP4, which argues an...
Chronic kidney disease (CKD) and periodontitis (PD) are serious public-health concerns. Vitamin D is a fat-soluble steroid hormone that interacts with its nuclear receptor (VDR) to regulate variety of biological processes, such as bone metabolism, immune response modulation transcription several genes involved in CKD PD mechanisms. The aim this work was investigate the association between polymorphisms VDR gene end-stage renal (ESRD) PD.222 subjects without ESRD (in hemodialysis) were...
Abstract Canova (CA) is a complex homeopathic medication used in diseases where the immune system depressed. Previous studies demonstrated that it neither toxic nor mutagenic and activates macrophages. We now evaluate CA effects on cytokine production gene expression from mice The global view of changes genes with known functions can provide vivid picture way which cell adapts to changing environment or challenge. found decrease IL‐2 IL‐4 differential 147 group. These are mainly involved...
In trypanosomatids, the regulation of gene expression occurs mainly at post‐transcriptional level. Previous studies have revealed nontranslated mRNA in Trypanosoma cruzi cytoplasm. Previously, we identified and cloned TcDHH1 protein, a DEAD box RNA helicase. It has been reported that Dhh1 is involved multiple RNA‐related processes various eukaryotes. also to accumulate stress granules processing bodies yeast, animal cells, brucei T. . localized discrete cytoplasmic foci vary depending on...
Abstract Background Simian malaria is still an open question concerning the species of Plasmodium parasites and New World monkeys susceptible to parasites. In addition, lingering as whether these animals are reservoirs for human might become important especially in a scenario eradication disease. To aid answers questions, were surveyed parasite natural infection Amazonian state Rondônia, Brazil, with intense environmental alterations due activities, which facilitated sampling animals....
CBA macrophages effectively control Leishmania major infection, yet are permissive to amazonensis. Employing a transcriptomic approach, we previously showed the up-regulation of genes involved in classical pathway macrophage activation resistant mice. However, microarray analyses do not evaluate changes gene expression that occur after translation. To circumvent this analytical limitation, employed proteomics approach increase our understanding modulations during infection and identify novel...
Trypanosoma cruzi is the protozoan parasite that causes Chagas disease or American trypanosomiasis. Kinetoplastid parasites could be considered as model organisms for studying factors involved in posttranscriptional regulation because they control gene expression almost exclusively at this level. The PUF (Pumilio/FBF1) protein family regulates mRNA stability and translation eukaryotes, several members have been identified trypanosomatids. We used a ribonomic approach to identify putative...