A5080549750- Microbial Metabolism and Applications
- Ruminant Nutrition and Digestive Physiology
- Helminth infection and control
- Milk Quality and Mastitis in Dairy Cows
- Aquaculture Nutrition and Growth
- Microbial infections and disease research
- Soil Management and Crop Yield
- Enzyme Production and Characterization
- Crop Yield and Soil Fertility
- Neurological diseases and metabolism
- Streptococcal Infections and Treatments
- Microbial Community Ecology and Physiology
- Parasite Biology and Host Interactions
- Genetically Modified Organisms Research
- Neonatal and Maternal Infections
- Pneumonia and Respiratory Infections
- Prion Diseases and Protein Misfolding
- Peptidase Inhibition and Analysis
- Meat and Animal Product Quality
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Probiotics and Fermented Foods
- Biotechnology and Related Fields
- Coccidia and coccidiosis research
- Aquaculture disease management and microbiota
Pontifícia Universidade Católica do Paraná
2012-2024
Pontifical Catholic University of Puerto Rico
2006-2020
Catholic University of America
2010
Universidade de São Paulo
2000-2009
Universidade Estadual da Paraíba
2007
University of Nebraska–Lincoln
1996-2006
This work reports the results of analyses three complete mycoplasma genomes, a pathogenic (7448) and nonpathogenic (J) strain swine pathogen Mycoplasma hyopneumoniae avian synoviae; genome sizes strains were 920,079 bp, 897,405 799,476 respectively. These genomes compared with other sequenced reported in literature to examine several aspects evolution. Strain-specific regions, including integrative conjugal elements, rearrangements alterations adhesin sequences observed M. strains, all these...
Chromobacterium violaceum is one of millions species free-living microorganisms that populate the soil and water in extant areas tropical biodiversity around world. Its complete genome sequence reveals ( i ) extensive alternative pathways for energy generation, ii ≈500 ORFs transport-related proteins, iii complex systems stress adaptation motility, iv widespread utilization quorum sensing control inducible systems, all which underpin versatility adaptability organism. The also contains but...
The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable colonizing intercellular spaces grasses such as rice sugar cane. genome H. strain SmR1 was sequenced annotated by Paraná State Genome Programme—GENOPAR. composed a circular chromosome 5,513,887 bp contains total 4,804 genes. sequence revealed that highly versatile microorganism with capacity...
Spiders of the Loxosceles genus are cosmopolitan, and their venom components possess remarkable biological properties associated with ability to act upon different molecules receptors. Accidents intermedia specimens recognized as a public health problem in south Brazil. To describe transcriptional profile L. gland, we generated wide cDNA library, its transcripts were functionally structurally analyzed. After initial analyses, 1843 expressed sequence tags (ESTs) produced readable sequences...
<ns4:p>Background The escalating threat of AMR demands a paradigm shift in antimicrobial prescribing practices. application programming interface (API) is conceived as an advanced system, integrating artificial intelligence and machine learning, to optimize clinical decision-making the context therapy. This study outlines development evaluation software, emphasizing its potential impact on stewardship. Methods API was meticulously constructed two phases. In initial phase, algorithm...
One-hundred sixty-eight group B streptococcal (GBS) isolates from a Brazilian hospital were phenotypically and genotypically characterized. Isolates recovered human sources April 2006 to May 2008 classified as either invasive, noninvasive, or colonizing isolates. Classical methods for serotyping antibiotic resistance profiling employed. Clonal groups also defined by pulsed-field gel electrophoresis (PFGE). Results showed that susceptibility beta-lactam antimicrobials was predominant among...
Herbaspirillum rubrisubalbicans was first identified as a bacterial plant pathogen, causing the mottled stripe disease in sugarcane. H. can also associate with various plants of economic interest non pathogenic manner.A 21 kb DNA region genome contains cluster 26 hrp/hrc genes encoding for type three secretion system (T3SS) proteins. To investigate contribution T3SS to plant-bacterial interaction process we generated mutant strains M1 carrying Tn5 insertion both hrcN and hrpE genes....
ABSTRACT The main pathogenic treponemes causing bovine digital dermatitis were identified from 17 infected herds in southern Brazil for the first time this study using PCR. We did not find a relationship between treponeme phylogroup composition and clinical classification. Treponema phagedenis was present all lesions. Rumen fluid implicated as reservoir location these pathogens.
The aim of this work was evaluating the agronomical and chemical characteristics 20 different genotypes maize used as silage. A complete randomized experimental design used, with three replicates per treatment. Each hybrid harvested when plants reached 1/3 milk line stage, using two cut heights: immediately above senescent leaves (whole-plant) below ear attachment (half-plant), all hybrids were ensiled in PVC silos. After 50 days ensiling, DM, CP, EE, ADF, NDF, ash estimated TDN determined....
ABSTRACT: The present study aimed to assess the antibiotic resistance in 54 indigenous Lactobacillus plantarum isolated from artisanal fermented sausages. confirmation of strain species was performed by multiplex-PCR assay. Antibiotic assessed disk diffusion (DD) and Minimum Inhibitory Concentration (MIC) methods. Of L. plantarum, 44 strains were genotypically confirmed as 3 pentosus. highest rates ampicillin streptomycin. susceptibility shown tetracycline, chloramphenicol penicillin G. None...
To determine the polymorphisms of prion protein gene in sheep from state Paraná, Brazil, 323 animals meat breeds (Suffolk, Hampshire Down, Texel, Ile de France, Dorper, Dorset, Santa Inês and crossbreds) were genotyped by restriction fragment length polymorphism (RFLP) analysis. The most frequent allele was ARQ, with a frequency 0.61, followed ARR (0.30). VRQ AHQ alleles present at very low frequencies (0.13 0.05 respectively), ARH not found. Seven genotypes identified (ARR/ARR, ARR/ARQ,...
A chemical mutagenesis protocol was used with the ruminal bacterium Prevotella ruminicola strain B(1)4 to generate mutant strains defective in peptidase activity. Compared wild-type parent strain, isolated mutants possessed 1/10 of enzyme activity responsible for cleavage glycine-arginine-4-methoxy-beta-naphthylamide (Gly-Arg-MNA). concomitant loss against arginine-arginine-4-methoxy-beta-naphthylamide (Arg-Arg-MNA) also observed. Both activities were similarly affected by various proteinase...
This report describes the transcription apparatus of Mycoplasma hyopneumoniae (strains J and 7448) synoviae, using a comparative genomics approach to summarize main features related control gene expression in mycoplasmas. Most transcription-related genes present three strains are well conserved among Some unique aspects mycoplasmas scarcity regulatory proteins mycoplasma genomes discussed.
The aim of this work was to evaluate the agronomical characteristics, chemical composition and removal potassium five different genotypes corn grown for silage. A split-plot complete randomized block design used, with three hybrids (DKB 390, AGX 8517, A-2560) two varieties (ALBianco, Piratininga), using cut heights (20 40 cm above soil level) four replications per treatment. After harvest, plants were analyzed characteristics (K). Milk production DM intake predicted each There an increase in...
The aim of this work was to develop a fast and accurate molecular approach allow early detection two RAPD groups S. aureus causing bovine mastitis. Seventy five isolates from infected animals were characterized by RAPD. Genomic fragments isolated the unique bands present in either group cloned sequenced. Based on DNA sequences, specific primers designed for simultaneous multiplex PCR clinical subclinical Results showed that these proposed set could be used detect various as well...
Growth of Prevotella ruminicola strains B14 (subsp. brevis) and D31d ruminicola), was inhibited by protamine, a polycationic, low molecular mass protein. Results showed that protamine has bacteriocidal effect when present in concentrations exceeding 30 μg ml−1. Protamine exerted its toxic effects disrupting the outer membrane, which demonstrated by: (i) an increased sensitivity to hydrophobic antibiotics (novobiocin monensin) (ii) release periplasmic enzyme alkaline phosphatase following...
Different alleles of the human and ovine prion protein gene correlate with a varying susceptibility to transmissible spongiform encephalopathies. However, pathogenic implications specific polymorphisms in bovine (PRNP) are only poorly understood. Previous studies on PRNP investigated common European North American cattle breeds. As consequence decades intensive breeding for traits, these modern breeds represent small fraction pool. In this study, we analysed native Brazilian Caracu breed,...
The purpose of the present study was to functionally evaluate influence superoxide radical-generating compounds on heterologous induction a predicted promoter region open reading frames for paraquat-inducible genes (pqi genes) revealed during genome annotation analyses Chromobacterium violaceum bacterium. A 388-bp fragment corresponding pqi gene C. amplified using specific primers and cloned into conjugative vector containing Escherichia coli lacZ without promoter. Assessments expression...
(With 1 Figure)The Chromobacterium violaceum is a Gram-negative, free-living betaproteobacterium that dominates variety of ecosystems in tropical and subtropical regions. Notably, several refined mechanisms related to remarkable exploitable adaptability have been revealed the genome prospecting this bacterium, including biological responses oxidative stress by predicting paraquat-inducible proteins ( Brazilian National Genome Project Consortium, 2003). In cited study, two open reading frames...
The widespread occurrence of anthelmintic-resistant gastrointestinal nematodes (GINs), particularly Haemonchus contortus, in sheep production systems has magnified the need to identify and develop alternative control strategies. Strategies include selection genetically GIN-resistant implementation biological parasite reduce dependence on anthelmintic drugs. In this study, we aimed establish molecular identity bacterial communities present abomasum classified as resistant or susceptible H. an...
Haemonchus contortus is an important parasite in sheep and the abuse of anti-helminthics has resulted a rapid resistance. Little known about abomasal microbiota relation to local parasitism. This study aimed identify bacteria abomasum sheep, through content, mucosa, adult H. parasites, high low Eight naturally infected with were classified as (n = 4) infection 4). Samples contents, parasites collected. pooled form six groups, three each level parasitism: infection/abomasal contents (HC),...
PCR analysis is a sensitive and specific tool to detect monitor microorganisms in complex environmental samples. The amplification of 16S ribosomal DNA sequences followed by gel electrophoresis under denaturing gradient (DGGE) has been powerful technique genetically evaluate microbial ecosystems. Changes rumen populations were investigated vitro using basal diet with different lipid sources. PCRs performed two sets primers order amplify rRNA sequences, the amplified fragments submitted DGGE...