A5046932014- Protist diversity and phylogeny
- Heat shock proteins research
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Phytoplasmas and Hemiptera pathogens
- Polyamine Metabolism and Applications
- thermodynamics and calorimetric analyses
- Insect symbiosis and bacterial influences
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Parasitic Infections and Diagnostics
- Plant nutrient uptake and metabolism
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogenic Bacteria Studies
- Vibrio bacteria research studies
- Fungal Biology and Applications
- Cocoa and Sweet Potato Agronomy
- Wastewater Treatment and Nitrogen Removal
- Microbial Metabolism and Applications
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
Universidade de São Paulo
2009-2022
University of Exeter
2015
European Bioinformatics Institute
2007
Wellcome Trust
2007
Broad Institute
2007
Virginia Tech
2007
Johns Hopkins University
2007
Universidade Estadual de Campinas (UNICAMP)
2004
Universidade Federal do Rio de Janeiro
2004
Universidade Federal de São Paulo
2003
We present a draft sequence of the genome Aedes aegypti, primary vector for yellow fever and dengue fever, which at approximately 1376 million base pairs is about 5 times size malaria Anopheles gambiae. Nearly 50% Ae. aegypti consists transposable elements. These contribute to factor 4 6 increase in average gene length sizes intergenic regions relative An. gambiae Drosophila melanogaster. Nonetheless, chromosomal synteny generally maintained among all three insects, although conservation...
Sensing light is the fundamental property of visual systems, with vision in animals being based almost exclusively on opsin photopigments [1]. Rhodopsin also acts as a photoreceptor linked to phototaxis green algae [2, 3] and has been implicated by chemical means sensor flagellated swimming zoospores fungus Allomyces reticulatus [4]; however, signaling mechanism these fungi remains unknown. Here we use combination genome sequencing molecular inhibition experiments light-sensing phenotype...
ABSTRACT Misfolding and aggregation of protein molecules are major threats to all living organisms. Therefore, cells have evolved quality control systems for proteins consisting molecular chaperones proteases, which prevent by either refolding or degrading misfolded proteins. DnaK/DnaJ GroES/GroEL the best-characterized chaperone in bacteria. In Caulobacter crescentus these machines products essential genes, both induced heat shock cell cycle regulated. this work, we characterized...
In response to elevated temperature, both prokaryotic and eukaryotic cells increase expression of a small family chaperones. The regulatory network that functions control the transcription heat shock genes in bacteria includes unique structural motifs promoter region these alternate sigma factors. One conserved motifs, inverted repeat CIRCE element, is found 5' many operons, including Caulobacter crescentus groESL operon. We report identification another C. operon containing two genes, hrcA...
Summary The extracytoplasmic function sigma factor σ T is the master regulator of general stress response in Caulobacter crescentus and controls expression its paralogue U . In this work we showed that PhyR NepR act, respectively, as positive negative regulators function. Biochemical data also demonstrated directly binds phosphorylated form PhyR. We described essential role histidine kinase gene CC3474, here denominated phyK , for ‐dependent genes resistance to conditions. Additionally, vivo...
Caulobacter crescentus has a single dnaK gene that is highly homologous to the hsp70 family of heat shock genes. Analysis cloned and sequenced shown deduced amino acid sequence could encode protein 67.6 kilodaltons 68% identical DnaK Escherichia coli 49% Drosophila human family. A partial open reading frame 165 base pairs 3' end encodes peptide 190 acids 59% DnaJ E. coli. Northern blot analysis revealed 4.0-kilobase mRNA fragment. Since coding region 1.89 kilobases, dnaJ may be transcribed...
Summary Sigma factors of the ECF subfamily are important regulators stress responses in bacteria. Analysis Caulobacter crescentus genome sequence has indicated presence 13 members ( e xtra c ytoplasmic f unction) subfamily, suggesting that these play an role C. physiology. This work describes characterization two highly similar sigma factors, σ U and T . The corresponding genes not essential under normal growth conditions absence does impair bacterial resistance to environmental stresses...
Caulobacter crescentus performs chemotaxis by short intermittent reversals of rotation its single polar flagellum. Tn5 insertions causing a general phenotype, an inability to reverse swimming direction and form large swarm colonies, have been mapped 8-kb region the C. genome. These mutations had different effects on methyl-accepting proteins (MCP), activities methyltransferase methylesterase. The insertion mutant SC1130 no cross-reacting MCP reduced levels activity Other mutants bearing...
ABSTRACT Xylella fastidiosa is a phytopathogenic bacterium that causes serious diseases in wide range of economically important crops. Despite extensive comparative analyses genome sequences pathogenic strains from different plant hosts, nonpathogenic have not been studied. In this report, we show X. strain J1a12, associated with citrus variegated chlorosis (CVC), when injected into and tobacco plants. Furthermore, DNA microarray-based comparison J1a12 9a5c, CVC highly had its completely...
Nitrogen uptake and metabolism are essential to microbial growth. Gat1 belongs a conserved family of zinc finger containing transcriptional regulators known as GATA-factors. These factors activate the transcription Catabolite Repression (NCR) sensitive genes when preferred nitrogen sources absent or limiting. Cryptococcus neoformans GAT1 is an ortholog Aspergillus nidulans AreA Candida albicans genes. In attempt define function this regulator in C. neoformans, we generated null mutants...
The search for enriched (aka over-represented or enhanced) ontology terms in a list of genes obtained from microarray experiments is becoming standard procedure system-level analysis. This tries to summarize the information focussing on classification designs such as Gene Ontology, KEGG pathways, and so on, instead individual genes. Although it well known statistics that association significance are distinct concepts, only former approach has been used deal with term enrichment problem.BayGO...
Alternative sigma factors of the extracytoplasmic function (ECF) subfamily are important regulators stress responses in bacteria and have been implicated control homeostasis compartment cell. This work describes characterization sigF, encoding 1 13 members this identified Caulobacter crescentus. A sigF-null strain was obtained shown to be severely impaired resistance oxidative stress, caused by hydrogen peroxide treatment, exclusively during stationary phase. Although sigF mRNA levels...
Three sets of antibacterial nitrofuran derivatives [set I, 5-R-substituted (Z)-2-(5-nitrofuran-2-ylmethylene)-3(2H)-benzofuranones (R = OCH3, H, CH3, C2H5, nC3H7, Cl, Br, CN, and NO2) their 2-hydroxyphenyl 2-acetoxyphenyl analogues; set II, (E)-1-(2-hydroxyphenyl)-3-(5-nitrofuryl)-2-propen-1-ones NO2); III, (E)-1-(2-acetoxyphenyl)-3-(5-nitrofuryl)-2-propen-1-ones CH3; NO2)] were prepared tested against a Gram-positive (Staphylococcus aureus, strain ATCC-25923) Gram-negative bacterium...
Abstract 1. The synthesis of heat shock protein 70 (Hsp70) mRNA and the expression Hsp70 in liver broiler chickens submitted to acute stress (35°C for 5 h) was investigated. 2. detected by SDS‐PAGE Western blot analysis using a polyclonal antiserum against Blastocladiella emersonii. specific signal analysed Northern as probe cDNA B. 3. An increase amount from first up fifth hour exposure. This accompanied an which peaked at 3 h. 4. study shows that induced liver, vivo, are time dependent,...
The sporulation stage of the aquatic fungus Blastocladiella emersonii culminates with formation and release to medium a number zoospores, which are motile cells responsible for dispersal fungus. presence in solution 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), potent selective inhibitor nitric oxide-sensitive guanylyl cyclases, completely prevented biogenesis zoospores. In addition, this compound was able significantly reduce cGMP levels, increase drastically during late sporulation,...
Abstract Over 40,000 sugarcane (Saccharum officinarum) consensus sequences assembled from 237,954 expressed sequence tags were compared with the protein and DNA other angiosperms, including genomes of Arabidopsis rice (Oryza sativa). Approximately two-thirds transcriptome have similar in Arabidopsis. These may represent a core set proteins or domains that are conserved among monocots eudicots probably encode for essential angiosperm functions. The remaining putative monocot-specific genetic...
Xylella fastidiosa is a phytopathogenic bacterium that responsible for diseases in many economically important crops. Although different strains have been studied, little known about X. stress responses. One of the better characterized responses bacteria heat shock response, which induces expression specific genes to prevent protein misfolding and aggregation promote degradation irreversibly denatured polypeptides. To investigate involved we performed whole-genome microarray analysis time...
Caulobacter crescentus cells respond to a sudden increase in temperature by transiently inducing the synthesis of several polypeptides. Two proteins induced, Hsp62 and Hsp70, were shown be analogous heat shock Escherichia coli, GroEL DnaK, respectively, immunological cross-reactivity with antibodies raised against E. coli proteins. Two-dimensional gel electrophoretic resolution extracts labeled [35S]methionine during led identification 20 distinct Hsps C. which are coordinately expressed,...
Caulobacter crescentussigma(E) belongs to the ECF (extracytoplasmic function) subfamily of RNA polymerase sigma factors, whose members regulate gene expression in response distinct environmental stresses. During physiological growth conditions, data indicate that sigma(E) is maintained reduced levels due action ChrR, a negative regulator rpoE and function. However, once bacterial cells are exposed cadmium, organic hydroperoxide, singlet oxygen or UV-A irradiation, transcription induced...