A5017150203- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Insect Resistance and Genetics
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Antimicrobial Peptides and Activities
- Nerve injury and regeneration
- Periodontal Regeneration and Treatments
- Enzyme-mediated dye degradation
- Sexual function and dysfunction studies
- Protein Hydrolysis and Bioactive Peptides
- biodegradable polymer synthesis and properties
- Silk-based biomaterials and applications
- Oral and gingival health research
- Microbial Community Ecology and Physiology
- Studies on Chitinases and Chitosanases
- Animal Genetics and Reproduction
- Microplastics and Plastic Pollution
- Nerve Injury and Rehabilitation
- Invertebrate Immune Response Mechanisms
- Catalysis for Biomass Conversion
- Pain Mechanisms and Treatments
Universidade Estadual do Oeste do Paraná
2015-2024
Universidade Estadual do Paraná
2020-2023
Universidade Estadual de Maringá
2000-2010
European Union of Medical Specialists
2008
The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable colonizing intercellular spaces grasses such as rice sugar cane. genome H. strain SmR1 was sequenced annotated by Paraná State Genome Programme—GENOPAR. composed a circular chromosome 5,513,887 bp contains total 4,804 genes. sequence revealed that highly versatile microorganism with capacity...
Abstract The filamentous Thermoascus aurantiacus fungus characterized by its thermophilic nature, is recognized as an exceptional producer of various enzymes with biotechnological applications. This study aimed to explore applications using polygalacturonase (PG) derived from the PI3S3 strain. PG production was achieved through submerged fermentation and subsequent purification via ion-exchange chromatography gel filtration methods. crude extract exhibited a diverse spectrum enzymatic...
A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues performed. Using statistical approach as a strategy for optimization, induction activity evaluated residual corn straw, improved so that optimum condition achieved high activities 1003U/mL. According our known, this study is first show levels induction. In addition, application an experimental design with microorganism induce has not...
The aim of this work was to characterize and apply a polygalacturonase Penicillium janthinellum new strain VI2R3M. obtained from P. VI2R3M incubated in cultures passion fruit peel partially purified by ion‐exchange chromatography gel filtration. enzyme showed relative molecular mass 102·0 kDa, maximum activity at pH 5·0, temperature 50°C, 100% stablity 50°C 80% 3·0–5·0. apparent Km, Vmax Kcat values for hydrolyzing polygalacturonic acid were 2·56 mg ml−1, 163·1 U mg−1 277 s−1 respectively....
Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) is an important pest for Brazilian sugarcane. In the present study, we detected two distinct spots in hemolymph from septic injured larvae (HDs1 and HDs2), which are separated by 2DE gel electrophoresis. Both were subjected to in-gel tryptic digestion MALDI-TOF/TOF analysis, revealed sequence VFGTLGSDDSGLFGK both HDs1 HDs2. This had homology 80% identity with specific Lepidoptera antimicrobial peptides called gloverins. Analyses...
To evaluate the neurotrophin mRNA expression and axon count in median nerve of Wistar rats submitted to neural mobilization (NM) after compression. Eighteen animals were randomly divided into G1 (nerve compression only), G2 (NM for 1 min), G3 3 min). For NM, anesthetized right scapula received mobilization, adapted as indicated humans, on alternate days, from third 13th postoperative (PO) day, totaling six days therapy. On 14th PO euthanized. Fragments nerve, distal procedure, removed...
Xylanase produced from the newly isolated Penicillium crustosum FP 11 and its potential in prebleaching of kraft pulp were evaluated using a statistical approach. A Plackett–Burman design (PBD) was carried out to select significant variables medium, these being NaNO3, KH2PO4, MgSO4, KCl, Fe2(SO4)3, yeast extract, corn stover, initial pH, liquid culture under static conditions for 6 d at 28 °C. Statistical analysis with central composite response surface methodology showed that 0.15% (w/v) 2%...
The objective of this study was to determine the best conditions produce invertase by Cunninghamella echinulata PA3S12MM and immobilize apply enzyme.The maximum production verified in 8 days cultivation at 28°C supplemented with 10 g L-1 apple peel, reaching 1054.85 U ml-1 . purified from DEAE-Sephadex column. derivative immobilized alginate-gelatin-calcium phosphate showed reusability >50% for 19 cycles. glutaraldehyde-chitosan greater thermostability a different pH. hydrolysis 15 ml...
Three different regions of the alpha s1-casein gene (CSN1S1) were investigated to determine frequencies major alleles for null, low, intermediate and high milk protein expression in a herd dairy goats raised southeastern region Brazil. Genomic DNA samples obtained from leukocytes 145 interest amplified through Polymerase Chain Reaction (PCR), then evaluated both agarose (O E allele) polyacrylamide gels (F allele). For better characterization F allele, PCR-RFLP (PCR-Restriction Fragment...
Chitinases are enzymes capable of hydrolysing the β-1,4 bonds chitin releasing chitooligosaccharides and N-acetylglucosamine widely used in food, pharmaceutical, agricultural industries. Microorganisms potential producers this enzyme; however, there no reports literature on production chitinase by fungi genus Thermothelomyces. Thus, work aimed to investigate extracellular using alternative carbon sources fungus isolated from soil, Thermothelomyces heterothallicus PA2S4T. The was cultivated a...
Insects are organisms extremely well adapted to diverse habitats, primarily due their innate immune system, which provides them with a range of cellular and humoral responses against microorganisms. Lepidoptera hemolymph proteins involved in known; however, there is lack knowledge about the sugarcane borer Diatraea saccharalis. In this present work, pest insect were studied by applying proteomic methodologies. Two-dimensional electrophoresis (2-DE) gels extracted from naive larvae challenged...
Ganoderma australe was studied to determine the composition of cell wall, and polysaccharide fraction SK5 obtained after freeze-thawing an aqueous 5% potassium hydroxide extraction. The monosaccharide revealed by gas chromatography-mass spectrometry showed 81.3% glucose, analyses 13C nuclear magnetic resonance spectroscopy confirmed a β-glucan with glycosidic links (1→3)-β type most likely 4-O substituted. In addition, biological effect from G. evaluated via in vitro cultures peritoneal...
Multiple sequence alignments of the Bombyx mori fibroin light chain gene (fib-L) from hybrids and Chinese Japanese strains demonstrated that 51.6% fib-L third intron is conserved. One these conserved segments, 41 bp long, contains CGTTATTATACATATT, which duplicated in B. Nd-s(D) mutant. In present work, electrophoretic mobility assays computational analyses revealed a major peak intrinsic bent DNA within segment undergoes breakage previously-described mutation. This result suggested this...
ORFs 10 and 14 from Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) were amplified, cloned sequenced.Nucleotide analysis of these genes those other baculoviruses showed that are highly conserved.The p10 protein BmMNPV ORF10 has 70 amino acid residues similar to the four known BmNPV strains.The ORF14 alignment a higher identity with baculovirus Autographa californica nucleopolyhedrovirus.The putative transmembrane domain in C-terminal region, which is baculoviruses.A phylogenetic...
In cultures of the sciarid Bradysia hygida Sauaia et Alves (Diptera : Sciaridae) oviposition is asynchronous which hinders grouping individually collected embryo samples to be used in biochemical analysis and correlate data obtained with predominant embryonic stage this sciarid. Here we describe a Feulgen staining method that allows staging collections B. hygida. The stained egg at different time intervals after (48-72, 72-96, 96-144 h) also led preliminary information about embryogenesis....
This study aimed at the sequence and analysis of mtDNA control region (CR) Diatraea saccharalis. The genome PCR amplification was performed using complementary primers to flanking regions Bombyx mori CR mitochondrial segment. sequencing revealed that amplified product 568 bp long, which smaller than observed for B. (725 bp). Within segment, a with 338 nucleotides identified as region, displayed high AT content (93.5%). D. saccharalis multiple alignment showed this had similarity Lepidoptera...