A5016396921- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Antibiotic Resistance in Bacteria
- Plant Pathogenic Bacteria Studies
- Virology and Viral Diseases
- Enzyme Catalysis and Immobilization
- Plant Pathogens and Fungal Diseases
- Plant Diversity and Evolution
- Soil Carbon and Nitrogen Dynamics
- Infectious Encephalopathies and Encephalitis
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 detection and testing
- Enzyme Production and Characterization
- Mycobacterium research and diagnosis
- Malaria Research and Control
- Plant Virus Research Studies
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Fungal Infections and Studies
- Aquaculture disease management and microbiota
Fundação Carlos Chagas
2015-2024
Fundação Oswaldo Cruz
2015-2024
Université Laval
2022-2024
Universidade Federal do Paraná
2013-2022
Universidade Federal de Santa Catarina
2017
The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable colonizing intercellular spaces grasses such as rice sugar cane. genome H. strain SmR1 was sequenced annotated by Paraná State Genome Programme—GENOPAR. composed a circular chromosome 5,513,887 bp contains total 4,804 genes. sequence revealed that highly versatile microorganism with capacity...
The Brazilian Atlantic Forest is one of the 25 biodiversity hot spots in world. Although diversity its fauna and flora has been studied fairly well, little known microbial communities. In this work, we analyzed ecosystem to determine bacterial biodiversity, using 16S rRNA gene sequencing, correlated changes deduced taxonomic profiles with physicochemical characteristics soil. DNAs were purified from soil samples, was amplified construct libraries. Comparison 754 independent sequences 10...
Abstract Background Metagenomics, the application of molecular genomics to consortia non-cultivated microbes, has potential have a substantial impact on search for novel industrial enzymes such as esterases (carboxyl ester hydrolases, EC 3.1.1.1) and lipases (triacylglycerol lipases, 3.1.1.3). In current work, lipase gene was identified from fosmid metagenomic library constructed with "prokaryotic-enriched" DNA fat-contaminated soil collected wastewater treatment plant. Results preliminary...
The rapid growth of the world's population demands an increase in food production that no longer can be reached by increasing amounts nitrogenous fertilizers. Plant promoting bacteria (PGPB) might alternative to use efficiency (NUE) important crops such wheat. Azospirillum brasilense is one most promising PGPB and wheat roots colonized A. a good model investigate molecular basis plant-PGPB interaction including improvement plant-NUE promoted PGPB.We performed dual RNA-Seq transcriptional...
Background Podocarpus lambertii (Podocarpaceae) is a native conifer from the Brazilian Atlantic Forest Biome, which considered one of 25 biodiversity hotspots in world. The advancement next-generation sequencing technologies has enabled rapid acquisition whole chloroplast (cp) genome sequences at low cost. Several studies have proven potential cp genomes as tools to understand enigmatic and basal phylogenetic relationships different taxonomic levels, well further probe structural functional...
Background Performing chloroplast DNA (cpDNA) isolation is considered a major challenge among different plant groups, especially conifers. Isolating chloroplasts in conifers by such conventional methods as sucrose gradient and high salt has not been successful. So far, plastid genome sequencing protocols for conifer species have based mainly on long-range PCR, which known to be time-consuming difficult implement. Methodology/Principal Findings We developed protocol cpDNA using three...
Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis. We have previously characterized the <200-nt RNA sub-populations contained in fungal extracellular vesicles (EVs) from Pb18 other pathogenic fungi. presently used RNA-seq strategy to compare <200- >200-nt fractions EVs isolated culture supernatants of Pb18, Pb3, Pb01. Shared mRNA sequences were related protein modification, translation, DNA metabolism/biogenesis, while those transport oxidation-reduction...
Sugarcane is an important agricultural product of Brazil, with a total production more than 500 million tons. Knowledge the bacterial community associated crops and soil status decisive step towards understanding how microorganisms influence crop productivity. However, most studies aim to isolate endophytic or rhizosphere bacteria plant by culture-dependent approaches. Culture-independent approaches allow comprehensive view entire communities in environment. In present study, we have used...
The fast reduction of prices DNA sequencing allowed rapid accumulation genome data. However, the process obtaining complete sequences is still very time consuming and labor demanding. In addition, data produced from various technologies or alternative assemblies remain underexplored to improve assembly incomplete sequences. We have developed FGAP, a tool for closing gaps draft that takes advantage different datasets. FGAP uses BLAST align multiple contigs against aiming find overlap gaps....
Molecular mechanisms of plant recognition and colonization by diazotrophic bacteria are barely understood. Herbaspirillum seropedicae is a Betaproteobacterium capable colonizing epiphytically endophytically commercial grasses, to promote growth. In this study, we utilized RNA-seq compare the transcriptional profiles planktonic maize root-attached H. SmR1 recovered 1 3 days after inoculation. The results indicated that nitrogen metabolism was strongly activated in rhizosphere...
The COVID-19 epidemic in Brazil experienced two major lineage replacements until mid-2021. first was driven by P.2, late 2020, and the second Gamma, early 2021. To understand how these SARS-CoV-2 lineages spread Brazil, we analyzed 11,724 genomes collected throughout country between September 2020 April Our findings indicate that P.2 probably emerged July Rio de Janeiro state Gamma November Amazonas state. Both states were main hubs of viral disseminations to other Brazilian locations. We...
A novel gene coding for a LipA-like lipase with 283 amino acids and molecular mass of 32 kDa was isolated characterized from metagenomic library prepared mangrove sediment the south Brazilian coast.LipA 52% identical to lipolytic enzyme an uncultured bacterium shared only low identities (≤31%) lipases/esterases cultivable microorganisms.Phylogenetic analysis showed that LipA, together orthologous protein bacterium, forms unique branch within family I true lipases, thereby constituting new...
Herbaspirillum rubrisubalbicans M1 causes the mottled stripe disease in sugarcane cv. B-4362. Inoculation of this cultivar with seropedicae SmR1 does not produce symptoms. A comparison genomic sequences these closely related species may permit a better understanding contrasting phenotype such as endophytic association and pathogenic life style. To achieve goal, we constructed suppressive subtractive hybridization (SSH) libraries to identify DNA fragments present one absent other. In parallel...
ABSTRACT Five thousand mutants of Herbaspirillum seropedicae SmR1 carrying random insertions transposon pTnMod-OGmKm lacZ were screened for differential expression LacZ in the presence naringenin. Among 16 whose was regulated by naringenin genes predicted to be involved synthesis exopolysaccharides, lipopolysaccharides, and auxin. These loci are probably establishing interactions with host plants.
Summary The genome of Azoarcus olearius DQS‐4 T , a N 2 ‐fixing Betaproteobacterium isolated from oil‐contaminated soil in Taiwan, was sequenced and compared with other strains. sequence showed high synteny sp. BH72, model endophytic diazotroph, but low five non‐plant‐associated strains ( CIB, EBN1, KH32C, A. toluclasticus MF63 PA01). Average Nucleotide Identity (ANI) revealed that shares 98.98% identity which should now be included the species . contained several genes related to plant...
RNA-binding proteins (RBPs) have important functions in the regulation of gene expression. RBPs play key roles post-transcriptional processes all eukaryotes, such as splicing regulation, mRNA transport and modulation translation decay. assemble into different mRNA-protein complexes, which form messenger ribonucleoprotein complexes (mRNPs). Gene expression trypanosomatids occurs mainly at level a role processes. However, functional characterization Trypanosoma cruzi has been impaired due to...
The SARS-CoV-2 variant of concern (VOC) Delta was first detected in India October 2020. imported cases the Brazil were identified April 2021 southern region, followed by more different regions during following months. By early September 2021, already dominant southeastern (87%), (73%), and northeastern (52%) Brazilian regions. This study aimed to understand spatiotemporal dissemination dynamics Brazil. To this end, we employed a combination maximum likelihood (ML) Bayesian methods...
Abstract Dengue virus serotype 2, genotype Cosmopolitan (DENV-2-GII), is one of the most widespread DENV strains globally. In USA, DENV-2 epidemics have been dominated by Asian-American (DENV-2-GIII), and first cases DENV-2-GII were only described in 2019, Peru, 2021 Brazil. To gain new information about circulation Brazil, we sequenced 237 confirmed sampled between March 2023 revealed that already present all geographic regions The phylogeographic analysis inferred was introduced at least...
A clone (LP001) expressing a new lipase gene was isolated from metagenomic library of the Brazilian Atlantic Forest soil. The DNA insert LP001 fully sequenced, and 38 ORFs were identified. Comparison ORFs, %G + C content organization with sequenced bacterial genomes suggested that fosmid belongs to an organism Acidobacteria phylum. Protein domain analysis inactivation by transposon insertion showed protein encoded ORF29 responsible for activity named LipAAc. purified LipAAc capable...
The species Phytobacter diazotrophicus and the associated genus were originally described by Zhanget al. [Arch Microbiol189 (2008), 431-439] on basis of few endophytic nitrogen-fixing bacteria isolated from wild rice (Oryza rufipogon) in China. In this study, we demonstrate that a number clinical isolates either literature, preserved culture collections, or obtained during 2013 multi-state sepsis outbreak Brazil also belong to same genus. 16S rRNA gene sequencing, multilocus sequence...