A5012019870- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- Coastal wetland ecosystem dynamics
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Cassava research and cyanide
- Plant Pathogenic Bacteria Studies
- Genomics and Phylogenetic Studies
- Plant responses to water stress
- Soybean genetics and cultivation
- Soil Carbon and Nitrogen Dynamics
- Microbial Community Ecology and Physiology
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Genetic and Environmental Crop Studies
- Plant and fungal interactions
- Plant Disease Resistance and Genetics
- Agricultural pest management studies
- Botanical Research and Chemistry
- Photosynthetic Processes and Mechanisms
- Hemoglobin structure and function
- Growth and nutrition in plants
- Plant Diversity and Evolution
- Aluminum toxicity and tolerance in plants and animals
James Hutton Institute
2016-2025
University of Leeds
2024
Agriculture and Agri-Food Canada
2012
Kazusa DNA Research Institute
2012
Western University
2012
Aarhus University
2012
Royal Botanic Garden Sydney
2012
Royal Botanic Gardens Victoria
2012
Louisiana State University Agricultural Center
2011
University of Dundee
2001-2010
Bacterial infection of interior tissues legume root nodules is controlled at the epidermal cell layer and closely coordinated with progressing organ development. Using spontaneous nodulating Lotus japonicus plant mutants to uncouple nodule organogenesis from infection, we have determined role 16 genes in these two developmental processes. We show that host-encoded mechanisms control three alternative entry processes operating epidermis, cortex single level. Single did not involve formation...
Nuclear-cytoplasmic partitioning and traffic between cytoplasmic nuclear compartments are fundamental processes in eukaryotic cells. Nuclear pore complexes mediate transport of proteins, RNAs ribonucleoprotein particles out the nucleus. Here we present positional cloning a plant nucleoporin gene, Nup133 , essential for symbiotic signal transduction pathway shared by Rhizobium bacteria mycorrhizal fungi. Mutation results temperature sensitive nodulation deficient phenotype absence...
Burkholderia sensu lato is a large and complex group, containing pathogenic, phytopathogenic, symbiotic non-symbiotic strains from very wide range of environmental (soil, water, plants, fungi) clinical (animal, human) habitats. Its taxonomy has been evaluated several times through the analysis 16S rRNA sequences, concantenated 4⁻7 housekeeping gene lately by genome sequences. Currently, division this group into Burkholderia, Caballeronia, Paraburkholderia, Robbsia strongly supported...
A β-glucoronidase (GUS)-marked strain of Herbaspirillum seropedicae Z67 was inoculated onto rice seedling cvs. IR42 and IR72. Internal populations peaked at over 10 6 log CFU per gram fresh weight by 5 to 7 days after inoculation (DAI) but declined 3 4 28 DAI. GUS staining most intense on coleoptiles, lateral roots, the junctions some main roots. Bacteria entered roots via cracks points root emergence, with cv. IR72 appearing be more aggressively infected than IR42. H. subsequently colonized...
Agriculturally important grasses such as sugar cane (Saccharum sp.), rice (Oryza sativa), wheat (Triticum aestivum) sorghum (Sorghum bicolor), maize (Zea mays), Panicum maximum, Brachiaria spp., and Pennisetum purpureum contain numerous diazotrophic bacteria, as, Acetobacter diazotrophicus, Herbaspirillum Azospirillum spp. These bacteria do not usually cause disease symptoms in the plants with which they are associated more of them, for example, A. obligate or facultative endo-phytes that...
Six closely related N2-fixing bacterial strains were isolated from surface-sterilized roots and stems of four different rice varieties. The identified as Serratia marcescens by 16S rRNA gene analysis. One strain, IRBG500, chosen for further analysis showed acetylene reduction activity (ARA) only when inoculated into media containing low levels fixed nitrogen (yeast extract). Diazotrophy IRBG500 was confirmed measurement 15N2 incorporation sequence the PCR-amplified fragment nifH. To examine...
Journal Article Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus Get access E.K. James, James 3 1Department Biological Sciences, University DundeeDundee DD14HN, UK 3To whom correspondence should be addressed. Fax: + 44 382 322318 Search for other works this author on: Oxford Academic PubMed Google Scholar V.M. Reis, Reis 2EMBRAPA-CNPAB, SeropédicaItaguai 23851-970, Rio de Janeiro, RJ, Brazil F.L. Olivares, Olivares J.I. Baldani, Baldani J. Döbereiner...
Burkholderia has only recently been recognized as a potential nitrogen-fixing symbiont of legumes, but we find that the origins symbiosis in are much deeper than previously suspected. We sampled 143 symbionts from 47 native species Mimosa across 1800 km central Brazil and found 98% were Burkholderia. Gene sequences defined seven distinct divergent complexes within genus The symbiosis-related genes formed deep Burkholderia-specific clades, each specific to complex, implying these diverged...
Symbiotic nitrogen fixation (SNF) by intracellular rhizobia within legume root nodules requires the exchange of nutrients between host plant cells and their resident bacteria. Little is known at molecular level about transporters that mediate such exchanges. Several mutants model Lotus japonicus have been identified develop with metabolic defects cannot fix efficiently exhibit retarded growth under symbiotic conditions. Map-based cloning defective genes in two mutants, sst1-1 sst1-2 (for...
• The ability of Burkholderia phymatum STM815 to effectively nodulate Mimosa spp., and fix nitrogen ex planta, was compared with that the known symbiont Cupriavidus taiwanensis LMG19424. Both strains were equally effective symbionts M. pudica, but nodules formed by had greater nitrogenase activity. shown have a broader host range across genus than LMG19424, nodulating 30 out 31 species, 21 these effectively. LMG19424 nodulated only nine species. GFP-marked variants used visualise presence...
Fourteen strains were isolated from nitrogen-fixing nodules on the roots of plants genus Mimosa growing in Taiwan, Brazil and Venezuela. On basis 16S rRNA gene sequence similarities, all previously shown to be closely related each other belong Burkholderia . A polyphasic approach, including DNA–DNA reassociation, whole-cell protein analysis, fatty acid methyl ester analysis extensive biochemical characterization, was used clarify taxonomic position these strains: 14 classified as...
ABSTRACT Twenty Mimosa -nodulating bacterial strains from Brazil and Venezuela, together with eight reference rhizobial two other β-rhizobial strains, were examined by amplified rRNA gene restriction analysis. They fell into 16 patterns formed a single cluster the known β-rhizobia, Burkholderia caribensis , phymatum tuberum . The 16S sequences of 15 20 determined, all shown to belong genus ; four distinct clusters could be discerned, isolated same host species usually clustering very...
• An extensive survey of nodulation in the legume genus Mimosa was undertaken two major biomes Brazil, Cerrado and Caatinga, both which there are high degrees endemicity genus. Nodules were collected from 67 70 spp. found. Thirteen species newly reported as nodulating. examined by light electron microscopy, all except for M. gatesiae had a structure typical effective nodules. The endosymbiotic bacteria nodules identified Burkholderia via immunolabelling with an antibody against phymatum...
Abstract Infection thread–dependent invasion of legume roots by rhizobia leads to internalization bacteria into the plant cells, which is one salient features root nodule symbiosis. We found that two genes, Nap1 (for Nck-associated protein 1) and Pir1 121F-specific p53 inducible RNA), involved in actin rearrangements were essential for infection thread formation colonization Lotus japonicus its natural microsymbiont, Mesorhizobium loti. nap1 pir1 mutants developed an excess uncolonized...