Eigo Fukai

ORCID: 0000-0002-9736-7743
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About
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Research Areas
  • Chromosomal and Genetic Variations
  • Plant Reproductive Biology
  • Plant Molecular Biology Research
  • Plant Virus Research Studies
  • Plant Disease Resistance and Genetics
  • Plant nutrient uptake and metabolism
  • Plant Pathogens and Fungal Diseases
  • Legume Nitrogen Fixing Symbiosis
  • Plant-Microbe Interactions and Immunity
  • Genetic Mapping and Diversity in Plants and Animals
  • Microbial Community Ecology and Physiology
  • Methane Hydrates and Related Phenomena
  • Gene expression and cancer classification
  • Marine and coastal ecosystems
  • Photosynthetic Processes and Mechanisms
  • Genomics and Phylogenetic Studies
  • Plant tissue culture and regeneration
  • Plant Physiology and Cultivation Studies
  • Agronomic Practices and Intercropping Systems
  • Plant Gene Expression Analysis
  • Plant and animal studies
  • Soil Moisture and Remote Sensing
  • Ocean Waves and Remote Sensing
  • Genetic diversity and population structure
  • Radio Wave Propagation Studies

Niigata University
2015-2024

Institute of Agrobiological Sciences
2008-2022

Kazusa DNA Research Institute
2010-2022

National Agriculture and Food Research Organization
2022

Aarhus University
2006-2022

National Institute of Genetics
2022

University of Tsukuba
2015

Aichi University of Education
2012

Japan Atomic Energy Agency
2012

Tohoku University
2001-2006

The whole genome of Jatropha curcas was sequenced, using a combination the conventional Sanger method and new-generation multiplex sequencing methods. Total length non-redundant sequences thus obtained 285 858 490 bp consisting 120 586 contigs 29 831 singlets. They accounted for ∼95% gene-containing regions with average G + C content 34.3%. A total 40 929 complete partial structures protein encoding genes have been deduced. Comparison other plant species indicated that 1529 (4%) putative...

10.1093/dnares/dsq030 article EN cc-by-nc DNA Research 2010-12-13

Abstract Infection thread–dependent invasion of legume roots by rhizobia leads to internalization bacteria into the plant cells, which is one salient features root nodule symbiosis. We found that two genes, Nap1 (for Nck-associated protein 1) and Pir1 121F-specific p53 inducible RNA), involved in actin rearrangements were essential for infection thread formation colonization Lotus japonicus its natural microsymbiont, Mesorhizobium loti. nap1 pir1 mutants developed an excess uncolonized...

10.1105/tpc.108.063693 article EN The Plant Cell 2009-01-01

Summary Long terminal repeat ( LTR ) retrotransposons are closely related to retroviruses, and their activities shape eukaryotic genomes. Here, we present a complete Lotus japonicus insertion mutant collection generated by identification of 640 653 new events following de novo activation the element retrotransposon 1 LORE http://lotus.au.dk ). Insertion preferences critical for effective gene targeting, exploit our large dataset analyse characteristics in this context. We infer mechanism...

10.1111/tpj.13243 article EN cc-by-nc-nd The Plant Journal 2016-06-20

Summary We established a gene tagging population of the model legume Lotus japonicus using an endogenous long terminal repeat (LTR) retrotransposon Retrotransposon 1 ( LORE1 ). The was composed 2450 plant lines, from which total 4532 flanking sequence tags were recovered by pyrosequencing. two‐dimensional arrangement population, together with use multiple identifier sequences in primers used to amplify regions, made it possible trace insertions back original lines. large‐scale detection new...

10.1111/j.1365-313x.2011.04826.x article EN The Plant Journal 2011-10-21

The effect of phosphorus (P) and nitrogen (N) additions on the Synechococcus cell cycle was tested with natural populations from Mediterranean Sea in summer. In absence stimulation, synchronized to light-dark cycle. DNA synthesis began around 1600, a maximum S-phase cells observed at dusk (2100), G(inf2)-phase 2400. Addition P (as PO(inf4)(sup3-)) caused, all cases, decrease fraction G(inf2) 1800, no change 2400, an increase 1200 next day, while addition N NO(inf3)(sup-)) had effect. We...

10.1128/aem.62.7.2527-2533.1996 article EN Applied and Environmental Microbiology 1996-07-01

The 24-nucleotides (nt) phased secondary small interfering RNA (phasiRNA) is a unique class of plant RNAs abundantly expressed in monocot anthers at early meiosis. Previously, 44 intergenic regions were identified as the loci for longer precursor 24-nt phasiRNAs (24-PHASs) rice genome. However, regulatory mechanism that determines spatiotemporal expression these has remained elusive. ETERNAL TAPETUM1 (EAT1) basic-helix-loop-helix (bHLH) transcription factor indispensable induction programmed...

10.1371/journal.pgen.1007238 article EN cc-by PLoS Genetics 2018-02-12

Methylation patterns of plants are unique as, in addition to the methylation at CG dinucleotides that occurs mammals, also non-CG sites. Genes methylated sites, but transposable elements (TEs) both and The role transcriptional silencing TEs is being extensively studied this time, only very rare transpositions have been reported when machineries compromised. To understand TE suppression plant development, we characterized rice mutants with changes chromomethylase gene, OsCMT3a. oscmt3a...

10.1111/tpj.12952 article EN The Plant Journal 2015-08-04

Abstract Chrysanthemums are one of the most industrially important cut flowers worldwide. However, their segmental allopolyploidy and self-incompatibility have prevented application genetic analysis modern breeding strategies. We thus developed a model strain, Gojo-0 ( Chrysanthemum seticuspe ), which is diploid self-compatible pure line. Here, we present 3.05 Gb chromosome-level reference genome sequence, covered 97% C. genome. The contained more than 80% interspersed repeats,...

10.1038/s42003-021-02704-y article EN cc-by Communications Biology 2021-10-07

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 155:89-101 (1997) - doi:10.3354/meps155089 Planktonic oligotrich ciliates in NW Mediterranean: growth rates and consumption by copepods Pérez MT, Dolan JR, Fukai E In May 1995, planktonic ciliate community of Ligurian Sea (NW Mediterranean) was dominated 3 Strombidium species: a mixotroph 16 µm length,...

10.3354/meps155089 article EN Marine Ecology Progress Series 1997-01-01

Fusarium oxysporum f.sp. conlutinans (Foc) is a serious root-invading and xylem-colonizing fungus that causes yellowing in Brassica oleracea. To comprehensively understand the interaction between F. B. oleracea, composition of xylem sap proteome non-infected Foc-infected plants was investigated both resistant susceptible cultivars using liquid chromatography-tandem mass spectrometry (LC-MS/MS) after in-solution digestion proteins. Whole genome sequencing Foc carried out generated predicted...

10.3389/fpls.2016.00031 article EN cc-by Frontiers in Plant Science 2016-02-01

Abstract Colonization of new habitats is expected to require genetic adaptations overcome environmental challenges. Here, we use full genome re-sequencing and extensive common garden experiments investigate demographic selective processes associated with colonization Japan by Lotus japonicus over the past ~20,000 years. Based on patterns genomic variation, infer details process where L. gradually spread from subtropical conditions much colder climates in northern Japan. We identify regions...

10.1038/s41467-019-14213-y article EN cc-by Nature Communications 2020-01-14

Transposable elements represent a large proportion of the eukaryotic genomes. Long Terminal Repeat (LTR) retrotransposons are very abundant and constitute predominant family transposable in plants. Recent studies have identified chromoviruses to be widely distributed lineage Gypsy elements. These contain chromodomains their integrases, which suggests preference for insertion into heterochromatin. In turn, this might contributed patterning heterochromatin observed host Despite potential...

10.1371/journal.pgen.1000868 article EN cc-by PLoS Genetics 2010-03-04

We have identified a low-copy-number retrotransposon family present in nine to 10 copies the Lotus japonicus model legume genome, and characterized its activity. LORE1 (Lotus 1) belongs Ty3-gypsy group of elements, is long terminal repeat (LTR) retrotransposon. Genetic mapping located elements gene-rich regions chromosomes, analysis native as well new insertion sites revealed integration outside highly repetitive sequences centromeres telomeres. Sequencing individual members several intact...

10.1111/j.1365-313x.2005.02534.x article EN The Plant Journal 2005-09-12

Abstract Soluble N-Ethylmaleimide Sensitive Factor Attachment Protein Receptor (SNARE) proteins are crucial for signal transduction and development in plants. Here, we investigate a Lotus japonicus symbiotic mutant defective one of the SNARE proteins. When symbiosis with rhizobia, growth was retarded compared that wild-type plant. Although formed nodules, these exhibited lower nitrogen fixation activity than wild type. The rhizobia were able to invade nodule cells, but enlarged symbiosomes...

10.1104/pp.112.200782 article EN cc-by PLANT PHYSIOLOGY 2012-08-02

As climatic changes occur over the coming decades, our scientific understanding of plant responses to environmental cues will become an increasingly important consideration in breeding agricultural crops. This review provides a summary literature regarding vernalization research Brassicaceae, covering both historical origins and current molecular mechanisms behind regulatory pathways involved subsequent inflorescence. We discuss evolutionarily conserved biology between model organism...

10.1071/cp16468 article EN Crop and Pasture Science 2017-06-20

Abstract The determinants of recognition specificity self-incompatibility in Brassica are SRK the stigma and SP11/SCR pollen, both which encoded S locus. nucleotide sequence analyses many alleles have identified several interspecific pairs haplotypes having highly similar sequences between B. oleracea rapa. These considered to be derived from common ancestors maintained same after speciation. In this study, genome structures three were compared by sequencing SRK, SP11/SCR, their flanking...

10.1534/genetics.104.037267 article EN Genetics 2006-04-20

Abstract In the long history of human relations with flowering cherry trees in Japan, ‘Somei-Yoshino’ occupies an exceptional position among a variety trees: it is self-incompatible interspecific hybrid but has been enthusiastically planted by grafting throughout due most likely to its flamboyant appearance upon full bloom. Thus, gives us rare opportunity trace and investigate occurrence distribution somatic mutations within single plant species through analysis genomes clonally propagated...

10.1093/dnares/dsae025 article EN cc-by DNA Research 2024-08-08
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