A5074963270- Legume Nitrogen Fixing Symbiosis
- Soybean genetics and cultivation
- Plant Reproductive Biology
- Turfgrass Adaptation and Management
- Allelopathy and phytotoxic interactions
- Agronomic Practices and Intercropping Systems
- Plant Physiology and Cultivation Studies
- Plant tissue culture and regeneration
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Plant pathogens and resistance mechanisms
- Genetic diversity and population structure
- Seed Germination and Physiology
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
- Seedling growth and survival studies
- Plant and animal studies
- GABA and Rice Research
- Phytoestrogen effects and research
- Cassava research and cyanide
University of Miyazaki
2015-2024
Fuji Chemical Industries (Japan)
2020
Saga University
2009-2017
Frontier Science Foundation
2010-2012
Kagoshima University
2009
University of California, Los Angeles
2009
Light is critical for supplying carbon to the energetically expensive, nitrogen-fixing symbiosis between legumes and rhizobia. Here, we show that phytochrome B (phyB) part of monitoring system detect suboptimal light conditions, which normally suppress Lotus japonicus nodule development after Mesorhizobium loti inoculation. We found number nodules produced by L. phyB mutants significantly reduced compared with WT Miyakojima MG20. To explore causes other than photoassimilate production,...
Zoysiais a warm-season turfgrass, which comprises 11 allotetraploid species (2n= 4x= 40), each possessing different morphological and physiological traits. To characterize the genetic systems of Zoysia plants to analyse their structural functional differences in individual accessions, we sequenced genomes using HiSeq MiSeq platforms. As reference sequence species, generated high-quality draft genome Z. japonica accession 'Nagirizaki' (334 Mb) 59,271 protein-coding genes were predicted. In...
Abstract We performed whole-genome Illumina resequencing of 198 accessions to examine the genetic diversity and facilitate use soybean resources identified 10 million single nucleotide polymorphisms 2.8 small indels. Furthermore, PacBio was performed, a total 2,033 structure variants were identified. Genetic analysis congregated into three subgroups (Primitive, World, Japan) showed possibility long relatively isolated history cultivated in Japan. Additionally, skewed regional distribution...
The phytohormone abscisic acid (ABA) is known to be a negative regulator of legume root nodule formation. By screening Lotus japonicus seedlings for survival on an agar medium containing 70 microM ABA, we obtained mutants that not only showed increased number but also enhanced nitrogen fixation. mutant was designated fixation1 (enf1) and confirmed monogenic incompletely dominant. low sensitivity ABA phenotype thought result from either decrease in the concentration plant's endogenous or...
Establishment of a nitrogen-fixing symbiosis between legumes and rhizobia not only requires sufficient photosynthate, but also the sensing ratio red to far (R/FR) light. Here, we show that R/FR light positively influences arbuscular mycorrhizal (AM) legume non-legume through jasmonic acid (JA) strigolactone (SL) signaling. The level AM colonization in high light-grown tomato Lotus japonicus significantly increased compared with determined for low plants. Transcripts JA-related genes were...
Mesorhizobium loti MAFF303099, a microsymbiont of the model legume Lotus japonicus, possesses cluster genes (tts) that encode type III secretion system (T3SS). In presence heterologous nodD from Rhizobium leguminosarum and flavonoid naringenin, we observed elevated expression tts several proteins into culture medium. Inoculation experiments with wild-type T3SS mutant strains revealed affected nodulation at species level within genus either positively (L. corniculatus subsp. frondosus L....
Abstract Colonization of new habitats is expected to require genetic adaptations overcome environmental challenges. Here, we use full genome re-sequencing and extensive common garden experiments investigate demographic selective processes associated with colonization Japan by Lotus japonicus over the past ~20,000 years. Based on patterns genomic variation, infer details process where L. gradually spread from subtropical conditions much colder climates in northern Japan. We identify regions...
Abstract Wild soybean (Glycine soja), the ancestor of cultivated (G. max), is a crucial resource for capturing genetic diversity species. In this study, we used set 78 genome-wide microsatellite markers to analyse and geographic differentiation patterns in global collection 2,050 G. soja accessions mini-core max stored two public seed banks. We observed notable reduction compared with identified close phylogenetic relationship between subpopulation located central China. Furthermore,...
Abstract Simple sequence repeats (SSR) have several positive attributes as genetic markers when compared with other DNA‐based marker systems. Because of their successful use for analysis in a number plant species, they could be similarly useful tools the study various Zoysia species. A genomic library enriched AG/TC motif was developed from japonica cv. ‘Asagake’ and screened by using 5′‐biotin‐labeled oligonucleotides (AG 20 ). As result sequencing 162 clones, total 119 clones were...
Several zoysiagrasses (Zoysia spp.) have been reported to leaf-epidermal salt glands, and it has suggested that gland density, secretion tolerance are positively correlated. The economically most important Zoysia species japonica Steud., matrella Merr., pacifica (Goudswaard) M. Hotta & Kuroki, among these, Z. is considered be the salt-tolerant. In this study, we investigated characterised accumulation of Na+ 48 accessions three species. We did not find any morphological differences in...
Zoysia Willd. include one of the best‐known turfgrasses worldwide owing to its adaptability in a wide range environments and tolerance abiotic stresses including soil salinity, acidity, cold, drought, heat. Here, we report complete chloroplast genome sequence matrella (L.) Merr. obtained by combination 454 pyrosequencing Sanger sequencing platforms. The entire maps as circular molecule 135,810 bp built with quadripartite organization: two inverted repeats (IRs) 20,960 separated large single...
Zoysia japonica Steudel, Z. matrella (L.) Merrill, and pacifica (Goudswaard) M. Hotta Kuroki [syn. Merr. var. Goudswaard] are native turfgrass species in Japan the most economically important of this genus. Interspecific crossing within is possible because similarity chromosome number (2 n = 4 x 40), ecotypes with intermediate traits found along borders. The objectives study were to characterize morphological determine genetic variation population structure among 248 accessions that we have...
Soybean seeds contain many antioxidants, including flavonoids and other phytochemicals. Isoflavone is a phytoestrogen that mimics estrogenic effects on target tissues also exerts antioxidant activity by sequestering free radicals. Despite cultivars developed to date, varietal differences in flavonoid content Japanese soybean accessions remain less well characterized. Here, we evaluated the seed of isoflavones, total flavonoids, phenolics 26 accessions. Next, each accession was examined using...
Plant hormones have been identified to be versatile signaling molecules essential for plant growth, development, and stress response. Their content levels vary depending on the species, they also change in response any external stimuli. Thus, simultaneous quantification of multiple is required understand physiology. Sensitive quantitative analysis using liquid chromatography-linked mass spectrometry (LC-MS/MS) has used detecting hormones; however, without stable isotopes yet established. In...
Introduction The soil houses a tremendous amount of micro-organisms, many which are plant parasites and pathogens by feeding off roots for sustenance. Such root often rely on plant-secreted signaling molecules in the rhizosphere as host guidance cues. Here we describe isolation characterization chemoattractant plant-parasitic root-knot nematodes ( Meloidogyne incognita , RKN). Methods Super-growing Root (SR) culture, consisting excised from legume species Lotus corniculatus L., was found to...
Light is critical for supplying carbon use in the energetically expensive process of nitrogen-fixing symbiosis between legumes and rhizobia. We recently showed that root nodule formation phyB mutants [which have a constitutive shade avoidance syndrome (SAS) phenotype] was suppressed white light, nodulation wild-type controlled by sensing R/FR ratio through jasmonic acid (JA) signaling. concluded cause reduced inhibition JA-Ile production root. Here we show shoot level higher than strain...
The objective of the National BioResource Project (NBRP) in Japan is to collect, conserve and distribute biological materials for life sciences research. project consists twenty-eight bioresources, including animal, plant, microorganism DNA resources. NBRP Lotus Glycine aims support development legume research through collection, conservation, distribution these bioresources. japonicus a perennial that grows naturally throughout widely used as model plant legumes because such advantages its...
Lotus japonicus is a model legume used by researchers worldwide to study nodulation and other aspects of biology. We investigated 15 morphological traits in large collection wild L. accessions collected Japan, also analyzed the genetic diversity among them, order develop core based on genome-wide SSR polymorphisms. found wide accessions. A total 321 putative alleles were detected using 25 primer pairs. The average observed expected heterozygosity scores for individual loci 0.014 0.772,...
Forty-seven wild accessions of Lotus japonicus Regal (Japanese trefoil) indigenous to Japan were investigated for nine morphological characters. Average temperature and annual precipitation negatively correlated with stem color seed weight. On the other hand, latitude was positively these traits. Consequently, from sites at higher latitudes low temperatures tend have dark red stems heavy seeds. Cluster analysis based on characters classified 47 into six major groups. I included four tall...
Background: Understanding the distribution of fatty acids in wild soybean accession collected Japan and relationship geographic environmental factors accumulation is fundamental for using accessions as resources future development selection new varieties with desired traits.Materials Methods: The acid composition seeds from 319 (Glycine soja) were evaluated gas chromatography.Results: distributions palmitate (16:0), stearate (18:0), oleate (18:1), linoleate (18:2) "-linolenate (18:3)...
Abstract Wild soybean ( Glycine soja ) is a valuable genetic resource for improvement. Seed composition profiles provide beneficial information the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess variation in free amino acid abundance seeds germplasm collected Japan. The content from 316 accessions ranged 0.965 5.987 mg/g seed dry weight (DW), representing 6.2-fold difference. Three acids had highest coefficient (CV): asparagine (1.15),...
Abstract Digital phenotyping, particularly the use of plant 3D models, is a promising method for high‐throughput evaluation. Although many recent studies on topic have been published, further research needed to apply it breeding and other related fields. In this study, using model phenotyping system we developed, reconstructed analyzed 20 accessions zoysiagrass ( Zoysia spp.), including three species their hybrid, over period 1 year. Artificial neural network with hidden layers was able...