A5069487591- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Escherichia coli research studies
- COVID-19 Clinical Research Studies
- Mycobacterium research and diagnosis
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Phytoplasmas and Hemiptera pathogens
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Leptospirosis research and findings
- Enzyme-mediated dye degradation
- Monoclonal and Polyclonal Antibodies Research
- Calcium signaling and nucleotide metabolism
- Animal Virus Infections Studies
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 detection and testing
Institute of Biomedical Science
2020-2025
Universidade de São Paulo
2014-2024
Petrobras (Brazil)
2024
University of Birmingham
2022
Harvard University
2022
Universidade Cidade de São Paulo
2017
National Institutes of Health
2009-2012
National Center for Biotechnology Information
2008-2012
Instituto Paulo Gontijo
2009
Ludwig Cancer Research
2008
Proteinaceous toxins are observed across all levels of inter-organismal and intra-genomic conflicts. These include recently discovered prokaryotic polymorphic toxin systems implicated in intra-specific They characterized by a remarkable diversity C-terminal domains generated recombination with standalone toxin-coding cassettes. Prior analysis revealed striking nuclease deaminase among the modules. We systematically investigated using comparative genomics, sequence structure...
ABSTRACT Background Sugarcane cultivars are polyploid interspecific hybrids of giant genomes, typically with 10–13 sets chromosomes from 2 Saccharum species. The ploidy, hybridity, and size the genome, estimated to have >10 Gb, pose a challenge for sequencing. Results Here we present gene space assembly SP80-3280, including 373,869 putative genes their potential regulatory regions. alignment single-copy in diploid grasses indicates that could resolve 2–6 (up 15) homo(eo)logs 99.1%...
Cell surface proteins are excellent targets for diagnostic and therapeutic interventions. By using bioinformatics tools, we generated a catalog of 3,702 transmembrane located at the human cells (human cell surfaceome). We explored genetic diversity surfaceome different levels, including distribution polymorphisms, conservation among eukaryotic species, patterns gene expression. integrating expression information from variety sources, were able to identify genes with restricted in normal...
Abstract Background Citrus canker is a disease that has severe economic impact on the citrus industry worldwide. There are three types of canker, called A, B, and C. The have different phenotypes affect species. causative agent for type A Xanthomonas citri subsp. , whose genome sequence was made available in 2002. fuscans aurantifolii strain B causes C Results We sequenced genomes strains to draft status. compared their genomic content X. other genomes, with special emphasis III secreted...
Mycobacterium bovis is the main causative agent of zoonotic tuberculosis in humans and frequently devastates livestock wildlife worldwide. Previous studies suggested existence genetic groups M. strains based on limited DNA markers (a.k.a. clonal complexes), evolution ecology this pathogen has been only marginally explored at global level. We have screened over 2,600 publicly available genomes newly sequenced four strains, gathering 1,969 from 23 countries least 24 host species, including...
Type VI secretion systems (T6SSs) are nanomachines used by bacteria to inject toxic effectors into competitors. The identity and mechanism of many remain unknown. We characterized a Salmonella T6SS antibacterial effector called Tlde1 that is in target-cell periplasm neutralized its cognate immunity protein (Tldi1). Microscopy analysis reveals cells expressing stop dividing lose cell envelope integrity. Bioinformatic uncovers similarities between the catalytic domain l,d-transpeptidases....
Proteases represent common targets in combating infectious diseases, including COVID-19. The 3-chymotrypsin-like protease (3CLpro) is a validated molecular target for COVID-19, and it key developing potent selective inhibitors inhibiting viral replication of SARS-CoV-2. In this review, we discuss structural relationships diverse subsites 3CLpro, shedding light on the pivotal role dimerization active site architecture substrate recognition catalysis. Our analysis bioinformatics other...
Abstract The pattern and process of evolution in the nef gene HIV-1 was analyzed within among patients. Using a maximum likelihood method that allows for variable intensity selection pressure codons, strong positive detected hemophiliac patient over 30 mo infection. By reconstructing allele substitution this using parsimony, synapomorphic amino acid changes separating each time point were found to have high probabilities being under selection, with selective coefficients at least 3.6%....
The endosymbiotic origin of eukaryotes brought together two disparate genomes in the cell. Additionally, eukaryotic natural history has included other events, phagotrophic consumption organisms, and intimate interactions with viruses endoparasites. These phenomena facilitated large-scale lateral gene transfer biological conflicts. We synthesize information from nearly decades genomics to illustrate how interplay between conflicts impacted emergence new adaptations eukaryotes. Using...
Nicotinamide adenine dinucleotide (NAD) is a ubiquitous cofactor participating in numerous redox reactions. It also substrate for regulatory modifications of proteins and nucleic acids via the addition ADP-ribose moieties or removal acyl groups by transfer to ADP-ribose. In this study, we use in-depth sequence, structure genomic context analysis uncover new enzymes substrate-binding NAD-utilizing metabolic macromolecular modification systems. We predict that Escherichia coli YbiA related...
Significance 5-Methylcytosine in DNA of eukaryotes, such as humans, is an important epigenetic mark. The recently characterized TET/JBP enzymes generate oxidized derivatives methylcytosine, hydroxy-, formyl-, and carboxymethylcytosine mammals, which serve further marks or intermediates for demethylation. Unlike animals, contain one to three TET genes, fungi, mushrooms rusts, display lineage-specific expansions with numerous are often associated a unique class transposable elements. We...
Mycobacterium bovis causes bovine tuberculosis and is the main organism responsible for zoonotic in humans. We performed sequencing, assembly annotation of a Brazilian strain M. named SP38, comparative genomics genomes deposited GenBank. SP38 has traditional tuberculous mycobacterium genome 4,347,648 bp, with 65.5% GC, 4,216 genes. The majority CDSs (2,805, 69.3%) have predictive function, while 1,206 (30.07%) are hypothetical. For analysis, 31 bovis, 32 BCG, 23 available GenBank were...
The type VI secretion system (T6SS) secretes antibacterial effectors into target competitors. Salmonella spp. encode five phylogenetically distinct T6SSs. Here, we characterize the function of SPI-22 T6SS bongori showing that it has activity and identify a group (TseV1–4) containing an N-terminal PAAR-like domain C-terminal VRR-Nuc encoded next to cognate immunity proteins with DUF3396 (TsiV1–4). TseV2 TseV3 are toxic when expressed in Escherichia coli bacterial competition assays confirm...
Herein, we present a novel esterase enzyme, Ade1, isolated from metagenomic library of Amazonian dark earths soils, demonstrating its broad substrate promiscuity by hydrolyzing ester bonds linked to aliphatic groups. The three-dimensional structure the enzyme was solved in presence and absence (tributyrin), revealing classification within α/β-hydrolase superfamily. Despite being monomeric enzymatic assays reveal cooperative behavior with sigmoidal profile (initial velocities vs...
The Leptospiraceae family, which includes the genera Leptospira, Leptonema, and Turneriella, is an ecologically diverse group that saprophytic strains from soil water as well important pathogenic strains. Adaptation to these multiple environments relies strongly on signal transduction adjust their morphology, motility, metabolism changing environmental conditions. Members of genus Leptospira distinguish themselves among spirochetes for having elevated number genes. In this study, we describe...
A tuberculose, uma das principais causas de morbidade e mortalidade no país, apresenta um panorama preocupante, com crescimento número casos diagnosticados. Entre os fatores que contribuem para o aumento da incidência, estão a condições socioeconômicas precárias, desigualdade acesso aos serviços saúde presença comorbidades como HIV diabetes. Além disso, resistência antimicrobiana dificuldade adesão ao tratamento são apontadas desafios significativos controle tuberculose Brasil. O objetivo...
Cell division in bacteria is carried out by an elaborate molecular machine composed of more than a dozen proteins and known as the divisome. Here we describe characterization new divisome protein Bacillus subtilis called YpsB. Sequence comparisons phylogentic analysis demonstrated that YpsB paralog site selection DivIVA. present several gram-positive likely originated from duplication DivIVA-like gene last common ancestor orders Bacillales Lactobacillales. We used green fluorescent...