A5013318827- Plant Pathogenic Bacteria Studies
- Protein Structure and Dynamics
- Bacterial Genetics and Biotechnology
- Legume Nitrogen Fixing Symbiosis
- Enzyme Structure and Function
- Plant nutrient uptake and metabolism
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Receptor Mechanisms and Signaling
- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Bacteriophages and microbial interactions
- Toxin Mechanisms and Immunotoxins
- Vibrio bacteria research studies
- Advanced NMR Techniques and Applications
- Plant and soil sciences
- Mass Spectrometry Techniques and Applications
- Ion channel regulation and function
- Microtubule and mitosis dynamics
- Machine Learning in Bioinformatics
- Advanced Polymer Synthesis and Characterization
- NMR spectroscopy and applications
- Photosynthetic Processes and Mechanisms
- Enzyme Production and Characterization
- RNA Interference and Gene Delivery
Universidade de São Paulo
2015-2025
Institute of Chemistry
2025
Universidade Brasil
2021
Texas A&M University – Kingsville
2014
University of Pennsylvania
2014
University of California, San Francisco
2014
University of Almería
2007-2013
Florida State University
2010-2012
National High Magnetic Field Laboratory
2010-2012
Institut Pasteur
2011
Type IV secretion systems (T4SSs) are multiprotein complexes that transport effector proteins and protein–DNA through bacterial membranes to the extracellular milieu or directly into cytoplasm of other cells. Many bacteria family Xanthomonadaceae, which occupy diverse environmental niches, carry a T4SS with unknown function but several characteristics distinguishes it from T4SSs. Here we show Xanthomonas citri provides these cells capacity kill Gram-negative species in contact-dependent...
The PilZ protein was originally identified as necessary for type IV pilus (T4P) biogenesis. Since then, a large and diverse family of bacterial homology domains have been identified, some which implicated in signaling pathways that control important processes, including motility, virulence biofilm formation. Furthermore, many domains, though not itself, shown to bind the second messenger bis(3′→5′)cyclic diGMP (c-diGMP). crystal structures orthologs from Xanthomonas axonopodis pv citri...
Type IV secretion systems (T4SS) are used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope into target cells. Translocation outer membrane is achieved via a ringed tetradecameric complex made up of small VirB7 lipoprotein (normally 30 45 residues in mature form) C-terminal domains VirB9 VirB10 subunits. Several species from genera Xanthomonas phytopathogens possess an uncharacterized type system with some distinguishing features, one which...
Glucokinase (GCK) catalyzes the rate-limiting step of glucose catabolism in pancreas, where it functions as body's principal sensor. GCK dysfunction leads to several potentially fatal diseases including maturity-onset diabetes young type II (MODY-II) and persistent hypoglycemic hyperinsulinemia infancy (PHHI). maintains homeostasis by displaying a sigmoidal kinetic response increasing blood levels. This positive cooperativity is unique because enzyme exclusively monomer possesses only single...
Signal transduction pathways mediated by cyclic-bis(3'→5')-dimeric GMP (c-di-GMP) control many important and complex behaviors in bacteria. C-di-GMP is synthesized through the action of GGDEF domains that possess diguanylate cyclase activity degraded EAL or HD-GYP with phosphodiesterase activity. There mounting evidence some c-di-GMP-mediated require protein-protein interactions between members GGDEF, EAL, PilZ protein domain families. For example, have been observed from FimX Xanthomonas...
The Leptospiraceae family, which includes the genera Leptospira, Leptonema, and Turneriella, is an ecologically diverse group that saprophytic strains from soil water as well important pathogenic strains. Adaptation to these multiple environments relies strongly on signal transduction adjust their morphology, motility, metabolism changing environmental conditions. Members of genus Leptospira distinguish themselves among spirochetes for having elevated number genes. In this study, we describe...
Specific ion effects in surfactant solutions affect the properties of micelles. Dodecyltrimethylammonium chloride (DTAC), bromide (DTAB), and methanesulfonate (DTAMs) micelles are typically spherical, but some organic anions can induce shape or phase transitions DTA+ Above a defined concentration, sodium triflate (NaTf) induces separation dodecyltrimethylammonium (DTATf) micelles, phenomenon rarely observed cationic This unexpected behavior DTATf/NaTf system suggests that DTATf aggregates...
The SARS-CoV-2 pandemic has already killed more than one million people worldwide. To gain entry, the virus uses its Spike protein to bind host hACE-2 receptors on cell surface and mediate fusion between viral membranes. As initial steps leading entry involve significant changes in conformation as well electrostatic environment vicinity of Spike/hACE-2 complex, we explored sensitivity interaction ionic strength through computational simulations plasmon resonance. We identified two regions...
High-resolution nuclear magnetic resonance is used to investigate the conformational dynamics of human glucokinase, a 52 kDa monomeric enzyme that displays kinetic cooperativity. 1H−15N transverse relaxation optimized spectra uniformly labeled recorded in absence and presence glucose, reveal significant cross-peak overlap heterogeneous peak intensities persist over range temperatures. 15N-specific labeling isoleucines tryptophans, reporting on backbone side chain dynamics, respectively,...
Chemical investigations of the terrestrial cyanobacterium Sphaerospermopsis torques-reginae ITEP-024 from northern Brazil afforded namalides B (1) and C (2), first analogues this anabaenopeptide-like metabolite to be described. Four other related peptides (3–6), termed spumigins K–N, were also identified. Planar structures absolute configurations for 1, 2, 3a–6a deduced by a combination 2D NMR, HRMS analysis, Marfey’s methodology. Spumigins K–N (3–6) are examples containing...
The objective of this study was to evaluate the effects two irrigation water qualities and three different levels nitrogen–phosphorous–potassium (NPK) rates applied by fertigation on yield quality tomato fruit. experiment carried out in a polyethylene greenhouse 'Pitenza' grown sand‐mulched sandy loam soil using trickle system. treatment consisted (C2S1 C3S2) fertilization levels: 50%, 100%, 200% [7.1 mmol L−1 nitrate (NO3) N, 0.5 P, 3.1 K] for each category. dry‐matter content fruit (DMC),...
Proline, betaine, and choline are protector molecules, related with secondary metabolism, that protect cells against oxidative damage by reactive oxygen species. They present in many organisms, including bacteria, fungi, plants, response to low water stress, salinity, availability of essential nutrients, diseases, others abiotic stress. The objective the work was determine effect phosphorus (P) (deficient, adequate, excessive, or toxic) levels on proline, protectors green bean plants...
Significance Type IV secretion systems (T4SSs) have been studied for more than 70 y because of their roles in mediating horizontal DNA transfer, responsible the spread antibiotic resistance, and injection virulence factors into animal plant hosts. Another important function is contact-dependent toxic effectors competing bacteria different species during bacterial warfare. The present study reveals how T4SSs use a specific domain VirD4 coupling protein to recruit antibacterial toxins by...
Leptospira interrogans serovar Copenhageni is a human pathogen that causes leptospirosis, worldwide zoonosis. The L. genome codes for wide array of potential diguanylate cyclase (DGC) enzymes with characteristic GGDEF domains capable synthesizing the cyclic dinucleotide c-di-GMP, known to regulate transitions between different cellular behavioral states in bacteria. Among such enzymes, LIC13137 (Lcd1), which has an N-terminal cGMP-specific phosphodiesterases, adenylyl cyclases, and FhlA...
The Na(+) /Ca(2+) exchanger provides a major Ca(2+) extrusion pathway in excitable cells and plays key role the control of intracellular concentrations. In Canis familiaris, (NCX) activity is regulated by binding to two cytosolic -binding domains, CBD1 CBD2, such that activates exchanger. Despite its physiological importance, little known about exchanger's global structure, mechanism allosteric -regulation remains unclear. It was found previously for NCX absence domains CBD2 loop are...
Type IV pili (T4P) are thin and flexible filaments found on the surface of a wide range Gram-negative bacteria that undergo cycles extension retraction participate in variety important functions related to lifestyle, defense pathogenesis. During pilus extensions, PilB ATPase energizes polymerization pilin monomers from inner membrane. In Xanthomonas citri , two cytosolic proteins, PilZ c-di-GMP receptor FimX, involved regulation T4P biogenesis through interactions with PilB. vivo...