A5082416165- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Banana Cultivation and Research
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Cancer-related gene regulation
- TGF-β signaling in diseases
- Enzyme Production and Characterization
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Galectins and Cancer Biology
- Developmental Biology and Gene Regulation
- dental development and anomalies
- Heat shock proteins research
- Viral gastroenteritis research and epidemiology
- Aquaculture disease management and microbiota
- Eosinophilic Esophagitis
- 14-3-3 protein interactions
- Salmonella and Campylobacter epidemiology
- Stress Responses and Cortisol
- Gastrointestinal motility and disorders
- Enzyme Structure and Function
Universidade Estadual de Campinas (UNICAMP)
2015-2024
Universidade de São Paulo
2005-2015
The University of Texas Health Science Center at Houston
2009-2012
Central University Hospital of Asturias
2011
Universidade Federal do Rio de Janeiro
2002-2005
Massachusetts Institute of Technology
1996
Type IV secretion systems (T4SSs) are multiprotein complexes that transport effector proteins and protein–DNA through bacterial membranes to the extracellular milieu or directly into cytoplasm of other cells. Many bacteria family Xanthomonadaceae, which occupy diverse environmental niches, carry a T4SS with unknown function but several characteristics distinguishes it from T4SSs. Here we show Xanthomonas citri provides these cells capacity kill Gram-negative species in contact-dependent...
Summary Sigma factors of the ECF subfamily are important regulators stress responses in bacteria. Analysis Caulobacter crescentus genome sequence has indicated presence 13 members ( e xtra c ytoplasmic f unction) subfamily, suggesting that these play an role C. physiology. This work describes characterization two highly similar sigma factors, σ U and T . The corresponding genes not essential under normal growth conditions absence does impair bacterial resistance to environmental stresses...
Type IV secretion systems (T4SS) are used by Gram-negative bacteria to translocate protein and DNA substrates across the cell envelope into target cells. Translocation outer membrane is achieved via a ringed tetradecameric complex made up of small VirB7 lipoprotein (normally 30 45 residues in mature form) C-terminal domains VirB9 VirB10 subunits. Several species from genera Xanthomonas phytopathogens possess an uncharacterized type system with some distinguishing features, one which...
Summary Plant‐associated bacteria of the genus Xanthomonas cause disease in a wide range economically important crops. However, their ability to persist environment is still poorly understood. Predation by amoebas represents major selective pressure bacterial populations environment. In this study, we show that X. citri type 6 secretion system (T6SS) promotes resistance predation soil amoeba Dictyostelium discoideum . We found an extracytoplasmic function (ECF) sigma factor (EcfK) required...
ABSTRACT The Enterococcus faecalis prg and pcf genes of plasmid pCF10 encode a type IV secretion system (T4SS) required for conjugative transfer. PrgJ is member the VirB4 family ATPases that are universally associated with T4SSs. Here, we report purified dimers displayed ATP binding hydrolysis activities. A nucleoside triphosphate (NTP) site mutation (K471E) slightly diminished but abolished in vitro blocked transfer vivo . As shown affinity pulldown assays, K471E mutant protein interacted...
Alternative sigma factors of the extracytoplasmic function (ECF) subfamily are important regulators stress responses in bacteria and have been implicated control homeostasis compartment cell. This work describes characterization sigF, encoding 1 13 members this identified Caulobacter crescentus. A sigF-null strain was obtained shown to be severely impaired resistance oxidative stress, caused by hydrogen peroxide treatment, exclusively during stationary phase. Although sigF mRNA levels...
The intron of the corticotropin-releasing hormone (corticoliberin; CRH) gene contains a sequence over 100 bp alternating purine/pyrimidine residues. We have used binding Z-DNA-specific antibody in metabolically active, permeabilized nuclei to study formation Z-DNA this at various levels transcription. In NPLC human primary liver carcinoma cell line, activation cAMP-dependent pathways increased level transcription, while adding glucocorticoids inhibited transcription CRH gene. These cells...
Several Xanthomonas species have a type IV secretion system (T4SS) that injects cocktail of antibacterial proteins into neighbouring Gram-negative bacteria, often leading to rapid lysis upon cell contact. This capability represents an obvious fitness benefit since it can eliminate competition while the liberated contents lysed bacteria could provide increase in local availability nutrients. However, production this Mega Dalton-sized molecular machine, with over hundred subunits, also imposes...
Endolysins are bacteriophage (or phage)-encoded enzymes that catalyse the peptidoglycan breakdown in bacterial cell wall. The exogenous action of recombinant phage endolysins against Gram-positive organisms has been extensively studied. However, outer membrane acts as a physical barrier when considering use to combat Gram-negative bacteria. This study aimed evaluate antimicrobial activity SAR-endolysin LysKpV475 bacteria single or combined therapies, using an permeabilizer (polymyxin B) and...
The Msx1 gene in mice has been proven to be induced by BMP (bone morphogenetic protein) proteins, and three binding sites for SMAD, an intracellular signalling transducer, have already identified its promoter. Gel shift analyses were performed they demonstrated that the consensus found very near transcription start site, a region designed BP (basal promoter), is functional nuclear proteins from 10.5, 11.5 13.5 dpc (days post-coitum) embryos. Notably, this occurs only when SMAD-binding...
Salmonella enterica Enteritidis forms biofilms and survives in agricultural environments, infecting poultry eggs. Bacteria are difficult to eradicate compared planktonic cells, causing serious problems industry public health. In this study, we evaluated the role of ihfA ihfB biofilm formation by S. employing different microbiology techniques. Our data indicate that ihf mutant strains impaired formation, showing a reduction matrix decrease viability metabolic activity. Phenotypic analysis...
Summary The heat shock response in Caulobacter crescentus was previously shown to be positively regulated by the alternative sigma factor of RNA polymerase (RNAP) σ 32 , and negatively modulated DnaK during induction phase but not recovery phase. In present work we have investigated involvement chaperone ClpB control C. . Data obtained indicated a role downregulation protein (HSP) synthesis, as cells lacking this showed prolonged shutoff response. Escherichia coli it has been proposed that...
The Xanthomonas genus comprises a large number of phytopathogenic species that infect wide variety economically important plants worldwide. Bacterial adaptation to the plant and soil environment relies on their repertoire signal transduction pathways, including alternative sigma factors extracytoplasmic function family (σ ECF ).
The phytopathogen Xanthomonas citri subsp (Xac) is responsible for big economic losses in agriculture.Several important processes to bacteria's survival are mediated by the translocation of macromolecules extracellular milieu or target cells.The type 6 Secretion System (T6SS) a recently described mechanism secrete proteins and usually associated interbacterial competition, but Xac this function known be dependent another secretion system, 4 (T4SS).In work, we have analyzed gene expression...
Type 6 secretion systems (T6SSs) are specialized multiprotein complexes that inject protein effectors into prokaryotic and/or eukaryotic cells. We previously described the role of T6SS phytopathogen Xanthomonas citri pv. as an anti-eukaryotic nanoweapon confers resistance to predation by amoeba Dictyostelium discoideum. Transcription X. genes is induced a signaling cascade involving Ser/Thr kinase PknS and extracytoplasmic function sigma factor EcfK. Here, we used strain overexpressing...
A bactéria Xanthomonas citri pv é o fitopatógeno causador do cancro cítrico.O projeto consiste na análise os efeitos de sinais químicos presentes em extratos plantas hospedeiras fisiologia da X.citri.Os resultados obtidos indicam que cultivo presença laranja induz