A5011982393- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- SARS-CoV-2 detection and testing
- Plant Parasitism and Resistance
- SARS-CoV-2 and COVID-19 Research
- Vibrio bacteria research studies
- Escherichia coli research studies
- Bacterial biofilms and quorum sensing
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Neurobiology and Insect Physiology Research
- Bacteriophages and microbial interactions
- Laser Applications in Dentistry and Medicine
- Plant Virus Research Studies
- Microbial infections and disease research
- Hereditary Neurological Disorders
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Pain Mechanisms and Treatments
- Artificial Intelligence in Healthcare
- Photodynamic Therapy Research Studies
- Digital Imaging for Blood Diseases
Universidade de São Paulo
2018-2024
Universidade de Ribeirão Preto
2020-2024
Universidade Federal de Santa Maria
2021
Instituto de Biología Molecular y Celular de Rosario
2012-2018
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2018
National University of Rosario
2012-2018
Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein system (T3SS). The role T3SS in pathogenesis is well established but whether this involved multicellular processes, such as biofilm formation has not been elucidated. Here, phytopathogen Xanthomonas citri subsp. (X. citri) was used model to gain further insights about formation.The capacity different X. mutants compared wild type strain and it observed that necessary for...
Summary X anthomonas axonopodis pv. citri ( ac), the bacterium that causes citrus canker, contains a gene in hrp [for hypersensitive response (HR) and pathogenicity] cluster encodes harpin protein called H pa1. pa1 produced HR nonhost plants tobacco, pepper A rabidopsis, whereas, host plant citrus, it elicited weak defence with no visible phenotype. Co‐infiltrations of ac or without recombinant leaves larger number cankers presence protein. To characterize effect during disease, an acΔhpa1...
Rapid diagnostics is pivotal to curb SARS-CoV-2 transmission, and saliva has emerged as a practical alternative naso/oropharyngeal (NOP) specimens. We aimed develop direct RT-LAMP (reverse transcription loop-mediated isothermal amplification) workflow for viral detection in saliva, provide more information regarding its potential curbing COVID-19 transmission. Clinical contrived specimens were used optimize formulations sample processing protocols. Salivary load was determined symptomatic...
Like several pathogenic bacteria, Xanthomonas infect host plants through the secretion of effector proteins by Hrp pilus Type Three Protein Secretion System (T3SS). HrpE protein was identified as major structural component this pilus. Here, using citri subsp. (Xcc) a model, novel role for an elicitor plant defense responses found. triggers in and non-host revealed development lesions, callose deposition, hydrogen peroxide production increase expression levels genes related to responses....
Several Xanthomonas species have a type IV secretion system (T4SS) that injects cocktail of antibacterial proteins into neighbouring Gram-negative bacteria, often leading to rapid lysis upon cell contact. This capability represents an obvious fitness benefit since it can eliminate competition while the liberated contents lysed bacteria could provide increase in local availability nutrients. However, production this Mega Dalton-sized molecular machine, with over hundred subunits, also imposes...
Type IV pili (T4P) are thin and flexible filaments found on the surface of a wide range Gram-negative bacteria that undergo cycles extension retraction participate in variety important functions related to lifestyle, defense pathogenesis. During pilus extensions, PilB ATPase energizes polymerization pilin monomers from inner membrane. In Xanthomonas citri , two cytosolic proteins, PilZ c-di-GMP receptor FimX, involved regulation T4P biogenesis through interactions with PilB. vivo...
Although the exact mechanism of pathogenesis coronavirus SARS-CoV-2 (COVID-19) is not fully understood, oxidative stress and release pro-inflammatory cytokines have been highlighted as playing a vital role in disease. In this sense, alternative treatments are needed to reduce level inflammation caused by COVID-19. Therefore, study aimed investigate potential effect red photobiomodulation (PBM) an attractive therapy downregulate cytokine storm COVID-19 zebrafish model. RT-qPCR analyses...
ABSTRACT There has been a growing interest in bacteriophages as therapeutic agents to treat multidrug-resistant bacterial infections. The present work aimed at expanding the microbiological and molecular characterization of lytic phages ZC01 ZC03 investigating their efficacy control Pseudomonas aeruginosa infection an invertebrate animal model. These two were previously isolated from composting using P. strain PA14 enrichment host had genomes sequenced. present, respectively, siphovirus...
Summary Establishing new experimental animal models to assess the safety and immune response antigen used in development of COVID-19 vaccine is an imperative issue. Based on advantages using zebrafish as a model research, herein we suggest doing this test putative candidates study against virus. We produced recombinant N-terminal fraction Spike SARS-CoV-2 protein injected it into adult female zebrafish. The specimens generated humoral immunity passed antibodies eggs. However, they presented...
Chronic pain is a major health issue, and the search for new analgesics has become increasingly important because of addictive properties unwanted side effects opioids. To explore potentially drug targets, we investigated mutations in NTRK1 gene found individuals with congenital insensitivity to anhidrosis (CIPA). encodes tropomyosin receptor kinase A (TrkA), nerve growth factor (NGF) that contributes nociception. Molecular modeling biochemical analysis identified decreased interaction...
Abstract Many bacteria kill rival species by translocating toxic effectors into target cells. Effectors are often encoded along with cognate immunity proteins that could (i) protect against “friendly-fire” ( trans -intoxication) from neighboring sister cells and/or (ii) internal cis -intoxication (suicide). Here, we distinguish between these two mechanisms in the case of bactericidal Xanthomonas citri Type IV Secretion System (X-T4SS). We use a set X. mutants lacking multiple...
Abstract Ca 2+ -regulated photoproteins (CaPhs) consist of single-chain globular proteins to which coelenterazine, a widely distributed marine luminogenic substrate (the luciferin), binds along with molecular oxygen, producing stable peroxide. Upon addition, CaPhs undergo conformational changes leading the cyclization peroxide and formation high-energy intermediate. Subsequently, its decomposition yields coelenteramide in an excited state results emission flash light. To date, all reported...
ABSTRACT Objectives Rapid diagnostics is pivotal to curb SARS-CoV-2 transmission, and saliva has emerged as a practical alternative naso/oropharyngeal (NOP) specimens. We aimed develop direct RT-LAMP workflow for viral detection in saliva, provide more information regarding its potential COVID-19 diagnostics. Methods Clinical contrived specimens were used screen/optimize formulations sample processing protocols. Salivary load was determined symptomatic patients evaluate clinical performance...
Abstract Many bacterial species use specialized secretion systems to translocate proteinaceous toxic effectors into target cells. In most cases, are encoded in bicistronic operons with their cognate immunity proteins. The current model is that proteins could, principle, provide protection two different ways: i) by avoiding self-intoxication (suicide or cis -intoxication) ii) inhibiting intoxication due “friendly-fire” translocation from neighboring sister cells (fratricide trans...