A5091492354- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Aquaculture Nutrition and Growth
- Animal Genetics and Reproduction
- Fish biology, ecology, and behavior
- Environmental Toxicology and Ecotoxicology
- Fish Ecology and Management Studies
- MicroRNA in disease regulation
- Microplastics and Plastic Pollution
- Natural product bioactivities and synthesis
- Nanoparticles: synthesis and applications
- Fish Biology and Ecology Studies
- Sexual Differentiation and Disorders
- SARS-CoV-2 and COVID-19 Research
- Chromosomal and Genetic Variations
- COVID-19 Clinical Research Studies
- Insect and Arachnid Ecology and Behavior
- Aquaculture disease management and microbiota
- Genetic diversity and population structure
- Birth, Development, and Health
- Effects and risks of endocrine disrupting chemicals
- Healthcare and Environmental Waste Management
- Pharmacological Effects of Natural Compounds
- Zebrafish Biomedical Research Applications
Universidade Estadual Paulista (Unesp)
2016-2025
University of South Bohemia in České Budějovice
2023
Universidade de São Paulo
2013-2022
Universidade Cidade de São Paulo
2022
Universidade Federal de Minas Gerais
2008-2021
Instituto Tecnológico de Chascomús
2021
Consejo Nacional de Investigaciones Científicas y Técnicas
2021
National University of General San Martín
2021
Instituto Federal Goiano
2021
Utrecht University
2009-2017
The zebrafish has become an important vertebrate model for basic and biomedical research, including the research field of biology reproduction. However, very few morphological stereological data are available regarding testis structure spermatogenesis. In this careful histomorphometric evaluation testis, we studied spermatogonial cells using molecular markers, determined combined duration meiotic spermiogenic phases, examined formation Sertoli cell barrier (tight junctions). We found at...
Background Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, and reside within a specific microenvironment in testes called “niche” which regulates cell properties, such as, self-renewal, pluripotency, quiescence their ability to differentiate. Methodology/Principal Findings Here, we introduce zebrafish as new model for study SSCs vertebrates. Using 5′-bromo-2′-deoxyuridine (BrdU), identified long term BrdU-retaining germ cells, type A undifferentiated spermatogonia...
This study aimed to improve, using the zebrafish model, our understanding of distinct roles pituitary gonadotropins FSH and LH in regulating testis functions teleost fish.We report, for first time a vertebrate species, that Leydig cells as well Sertoli express mRNAs both gonadotropin receptors (fshr lhcgr).Although cell fshr expression has been reported other piscine species may be common feature fish, lhcgr not previously might related undifferentiated gonochoristic mode gonadal sex...
Growth factors modulate germ line stem cell self-renewal and differentiation behavior. We investigate the effects of Igf3, a fish-specific member igf family. Fsh increased in steroid-independent manner number mitotic index single type A undifferentiated spermatogonia clones differentiating adult zebrafish testis. All 4 gene family members are expressed testis but tissue culture only igf3 transcript levels response to recombinant Fsh. This occurred cAMP/protein kinase A-dependent manner, with...
Anti-Müllerian hormone (Amh) is in mammals known as a TGFβ type of glycoprotein processed to yield bioactive C-terminal homodimer that directs regression Müllerian ducts the male fetus and regulates steroidogenesis early stages folliculogenesis. Here, we report on zebrafish Amh homologue. Zebrafish, all teleost fish, do not have ducts. Antibodies raised against N- part were used study processing endogenous recombinant Amh. The N-terminally directed antibody detected 27-kDa protein, whereas...
Glycoprotein hormone receptors mediate a diverse range of physiological functions in vertebrate and invertebrate organisms. The heterodimeric glycoprotein GPA2/GPB5 its receptor LGR1, constitute recently-discovered neuroendocrine signaling system that remains to be functionally characterized. We previously reported LGR1 is expressed the testes adult Aedes aegypti mosquitoes, where immunoreactivity particularly regionalized. Here, we show associated with centriole adjunct spermatids observed...
The study of sex determination and chromosome organization in nonmodel species has long been technically challenging, but new sequencing methodologies now enable precise high-throughput identification sex-specific genomic sequences. In particular, restriction site-associated DNA (RAD-Seq) is being extensively applied to explore systems many plant animal species. However, software specifically designed search for visualize sex-biased markers using RAD-Seq data lacking. Here, we present...
Thyroid hormones participate in regulating growth and homeostatic processes vertebrates, including development adult functioning of the reproductive system. Here we report a new stimulatory role thyroid hormone on proliferation Sertoli cells (SCs) single, type A undifferentiated spermatogonia (A(und)) zebrafish testes. for T3 testis is suggested by situ hybridization studies, which localized receptor α (thrα) SCs β (thrβ) mRNA Leydig cells. Using primary tissue culture system, effect steroid...
Impaired wound healing is a debilitating complication of diabetes that leads to significant morbidity, particularly foot ulcers. Natural products have shown be effective in treating skin wounds. Lupeol known stimulate angiogenesis, fibroblast proliferation, and expressions cytokines growth factors involved healing. The study performed evaluate the activity lupeol streptozotocin-induced hyperglycemic rats by macroscopical, histological, immunohistochemical, immunoenzymatic, molecular methods....
Skin wound healing is a highly complex event that involves different mediators at the cellular and molecular level. Lupeol has been reported to possess biological activities, such as anti-inflammatory, antioxidant, antidiabetic, in vitro properties, which motivated us proceed with vivo studies. We aimed investigate effect of lupeol-based cream for 3, 7, 14 days. Wound excisions were induced on thoraco-lumbar region rats topically treated immediately after injury induction. Macroscopic,...
Abstract Distinct types of oogonia are found in the germinal epithelium that borders ovarian lamellae Pimelodus maculatus : A‐undifferentiated, A‐differentiated and B‐oogonia. This is similar to situation observed for spermatogonia vertebrate testis. The single A‐undifferentiated divide by mitosis giving rise A‐groups differentiated oogonia, each enclosed epithelial cells prefollicle cells. Subsequently, proliferate generate B‐oogonia interconnected cytoplasmic bridges, hence, forming...
Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells chemical intermediates are involved. This study aimed to investigate the skin wound potential of menthol, as well mechanisms involved its effect, after 3, 7, or 14 days treatment, according phases healing. Skin was performed back Wistar rats, were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); menthol-based at 0.25%, 0.5%, 1.0% over days. Menthol 0.5% accelerated right...
Sex ratio depends on sex determination mechanisms and is a key demographic parameter determining population viability resilience to natural anthropogenic stressors. There increasing evidence that the environment can alter even in genetically sex-determined species (GSD), as elevated temperature cause female-to-male reversal (neomales). Alarmingly, neomales are being discovered populations of several fish, amphibian reptile worldwide. Understanding basis neomale development important for...
Stress is recognized as an adaptive response to potentially harmful environmental stimuli. The primary physiological adaptation stress increase in circulating cortisol levels, which, excess, can be transferred and incorporated into the oocytes of maturing females, affecting embryonic developmental program. Additionally, maternal energy availability essential factor that modulates this Based on background, we investigated effects development somatotropic axis zebrafish offspring juveniles....