A5006909645- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Epigenetics and DNA Methylation
- Aquaculture disease management and microbiota
- MicroRNA in disease regulation
- Birth, Development, and Health
- Physiological and biochemical adaptations
- Animal Genetics and Reproduction
- Fish Ecology and Management Studies
- Zebrafish Biomedical Research Applications
- Down syndrome and intellectual disability research
- Cancer-related gene regulation
- Gene expression and cancer classification
- Fish Biology and Ecology Studies
- Dementia and Cognitive Impairment Research
- Demographic Trends and Gender Preferences
- Latin American Urban Studies
- Animal Behavior and Reproduction
- Circular RNAs in diseases
- Spaceflight effects on biology
- Frailty in Older Adults
- Genetic and phenotypic traits in livestock
- Acute Ischemic Stroke Management
- Meat and Animal Product Quality
Institut de Ciències del Mar
2015-2025
Consejo Superior de Investigaciones Científicas
2015-2025
Institut Català de Ciències del Clima
2015-2025
Biomedical Research Networking Center on Neurodegenerative Diseases
2022-2024
Hospital de Sant Pau
2022-2024
Universitat Autònoma de Barcelona
2004-2024
Universidade de Santiago de Compostela
2014
Imperial College London
2008-2009
Government of Catalonia
2007
Sex ratio shifts in response to temperature are common fish and reptiles. However, the mechanism linking during early development sex ratios has remained elusive. We show European sea bass (sb), a which effects on maximal before gonads form, that juvenile males have double DNA methylation levels of females promoter gonadal aromatase (cyp19a), enzyme converts androgens into estrogens. Exposure high increased cyp19a females, indicating induced-masculinization involves methylation-mediated...
Amphibians are experiencing a panzootic of unprecedented proportions caused by the emergence Batrachochytrium dendrobatidis (Bd). However, all species not equally at risk infection, and is further modified environmental variables, specifically temperature. In order to understand how, when, hosts mount response Bd we analysed infection dynamics patterns gene expression in model amphibian Silurana (Xenopus) tropicalis. Mathematical modelling demonstrate existence temperature-dependent...
Abstract The zebrafish ( D anio rerio ) is a freshwater teleost of the family C yprinidae that established as model organism in many research fields. Here, we define characteristics fish species should have to serve for finfish aquaculture and argue fulfils essentially most them. We first describe several aspects biology including phylogenetic relationships, development growth reproduction, both wild under laboratory conditions. Next, review work already carried out related different...
Throughout the primary literature and within textbooks, erythrocyte has been tacitly accepted to have maintained a unique physiological role; namely gas transport exchange. In non-mammalian vertebrates, nucleated erythrocytes are present in circulation throughout life cycle fragmented series of observations mammals support potential role non-respiratory biological processes. We hypothesised that could actively participate via ligand-induced transcriptional re-programming immune...
Significance Fish exhibit remarkable sexual plasticity. However, the underlying mechanism of heat-induced sex reversal is still unclear. Here we first established conditions for reprogramming phenotypes in zebrafish through ratio analysis and gonad transcriptomics. Sex response to heat was family-specific resulted masculinization. We observed two gonadal transcriptomic profiles per adults, among them neomales and, strikingly, females with an ovary but a “male-like” transcriptome. The latter...
The zebrafish (Danio rerio) has become a well-established experimental model in many research fields but the loss of primary sex-determining region during process domestication renders laboratory strains susceptible to effects environmental factors on sex ratios. Further, an essential husbandry aspect - optimal rearing density avoid stress-induced masculinization is not known. We carried out two experiments: first focusing survival, growth and ratio by at different initial densities (9, 19,...
The marine environment is predicted to become warmer and more hypoxic, these conditions may a challenge for fish species. Phenotypically plastic responses facilitating acclimatization changing environments can be mediated by DNA methylation through the modulation of gene expression. To investigate whether temperature hypoxia exposure induce changes, we challenged post-smolt Atlantic salmon ( Salmo salar ) increasing temperatures (12 → 20°C, 1°C week –1 under normoxia or moderate (∼70% air...
Understanding how gilthead sea bream, Sparus aurata L., an important Mediterranean Sea species for aquaculture, respond physiologically to stressors commonly encountered in intensive rearing is effective production, as managing stress a major factor maintaining healthy fish stocks. Our objective was determine whether holding juvenile bream at high density (HD), chronic stressor, would affect their physiological responses subsequent acute handling stressor. After acclimation low (LD) of 6 kg...
In the wild, water temperature cycles daily: it warms up after sunrise, and cools rapidly sunset. Surprisingly, impact of such daily thermocycles during early development fish remains neglected. We investigated influence constant vs in zebrafish, from embryo to sexual differentiation, by applying four regimens: two (24°C 28°C) thermocycles: 28:24°C, TC (thermophase coinciding with daytime, cryophase night-time) 24:28°C, CT (opposite TC) a 12:12 h light:dark cycle (LD). Embryo was...
Epigenetics integrates genomic and environmental information to produce a given phenotype. Here, the model of Conserved Epigenetic Regulation Sex (CERS) is discussed. This based on our knowledge genes involved in sexual development epigenetic regulation gene expression activation silencing. was recently postulated be applied fish, it states that patterns are more associated with particular gonadal phenotype, e.g., testis differentiation, rather than intrinsic or extrinsic causes lead this...
Abstract Background Transcriptomic analysis is crucial for understanding the functional elements of genome, with classic method consisting screening transcriptomics datasets differentially expressed genes (DEGs). Additionally, since 2005, weighted gene co-expression network (WGCNA) has emerged as a powerful to explore relationships between genes. However, an approach combining both methods, i.e., filtering transcriptome dataset by DEGs or other criteria, followed WGCNA (DEGs + WGCNA), become...
Background Individuals of many vertebrate species show different stress coping styles and these have a striking influence on how gene expression shifts in response to variety challenges. Principal Findings This is clearly illustrated by study which common carp displaying behavioural predictors (characterised proactive, adrenaline-based or reactive, cortisol-based response) were subjected inflammatory challenge specific transcripts measured individual brains. Proactive reactive fish differed...
The role of epigenetic modifications such as DNA methylation during vertebrate sexual development is far from being clear. Using the zebrafish model, we tested effects one most common methyltransferase (dnmt) inhibitor, 5-aza-2′-deoxycytidine (5-aza-dC), which approved for treatment acute myeloid leukaemia and under active investigation solid tumours. Several dose–response experiments were carried out two periods, including not only very first days (0–6 post-fertilization, dpf), done in...
Sex ratio depends on sex determination mechanisms and is a key demographic parameter determining population viability resilience to natural anthropogenic stressors. There increasing evidence that the environment can alter even in genetically sex-determined species (GSD), as elevated temperature cause female-to-male reversal (neomales). Alarmingly, neomales are being discovered populations of several fish, amphibian reptile worldwide. Understanding basis neomale development important for...
Alzheimer disease (AD) is the main medical problem in adults with Down syndrome (DS). However, associations of age, intellectual disability (ID), and clinical status progression longitudinal cognitive decline have not been established.To examine along AD continuum its related to explore presence practice effects floor repeated assessments.This a single-center cohort study (aged >18 years) DS different ID levels at least 6 months follow-up between November 2012 December 2021. The data are...
Abstract Fish disease outbreaks caused by bacterial burdens are responsible for decreasing productivity in aquaculture. Unraveling the molecular mechanisms activated gonads after infections is pivotal enhancing husbandry techniques fish farms, ensuring management, and selecting most resilience phenotype. The present study, with an important commercial species European sea bass ( Dicentrarchus labrax ), Europe, examined changes miRNome transcriptome 48 h intraperitoneal infection Vibrio...
The response of the trout, O. mykiss, head kidney to bacterial lipopolysaccharide (LPS) or active and attenuated infectious hematopoietic necrosis virus (IHNV attINHV respectively) intraperitoneal challenge, 24 72 hours post-injection, was investigated using a salmonid-specific cDNA microarray. i.p. LPS-induced inflammation in first instance displays an initial stress reaction involving suppression major cellular processes, including immune function, followed by proliferative...
Abstract Background Genomic resources for plant and animal species that are under exploitation primarily human consumption increasingly important, among other things, understanding physiological processes establishing adequate genetic selection programs. Current available techniques high-throughput sequencing have been implemented in a number of species, including fish, to obtain proper description the transcriptome. The objective this study was generate comprehensive transcriptomic database...
The sex ratio is a key ecological demographic parameter crucial for population viability. However, the epigenetic mechanisms operating during gonadal development regulating gene expression and remain poorly understood. Moreover, there interest in of markers associated with particular phenotype or as sentinels environmental effects. Here, we profiled DNA methylation 10 genes related to stress, including steroidogenic enzymes, growth transcription factors. We provide novel information on...