A5021932811- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA and protein synthesis mechanisms
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Machine Learning in Bioinformatics
- Genetic diversity and population structure
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Fish Biology and Ecology Studies
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Genomics and Phylogenetic Studies
- Fish Ecology and Management Studies
- Protein Kinase Regulation and GTPase Signaling
- Genetic and phenotypic traits in livestock
- Prenatal Screening and Diagnostics
- Neuroendocrine regulation and behavior
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Cancer-related Molecular Pathways
- Cellular transport and secretion
Inserm
2010-2022
Université de Montpellier
2011-2022
Institut de Génomique Fonctionnelle
2011-2022
Centre National de la Recherche Scientifique
2011-2022
Montpellier GenomiX
2009-2020
Institut Cellule Souche et Cerveau
2015
Université Claude Bernard Lyon 1
2015
Laboratoire de Génétique Cellulaire
2013-2014
Laboratoire de Biologie du Développement
2010
Genopole (France)
2006
With more than 30,000 species, ray-finned fish represent approximately half of vertebrates. The evolution was impacted by several whole genome duplication (WGD) events including a teleost-specific WGD event (TGD) that occurred at the root teleost lineage about 350 million years ago (Mya) and recent in salmonids, carps, suckers others. In plants animals, are associated with adaptive radiations evolutionary innovations. WGD-spurred innovation may be especially relevant case fish, which...
The imprinted H19 gene produces a non-coding RNA of unknown function. Mice lacking show an overgrowth phenotype, due to cis effect the locus on adjacent Igf2 gene. To explore function itself, we produced transgenic mice overexpressing H19. We observed postnatal growth reduction in two independent lines and detected decrease Igf2expression embryos. An extensive analysis several other genes from newly described network (IGN) was performed both loss- gain-of-function animals. found that...
Abstract Sturgeons seem to be frozen in time. The archaic characteristics of this ancient fish lineage place it a key phylogenetic position at the base ~30,000 modern teleost species. Moreover, sturgeons are notoriously polyploid, providing unique opportunities investigate evolution polyploid genomes. We assembled high-quality chromosome-level reference genome for sterlet, Acipenser ruthenus . Our analysis revealed very low protein rate that is least as slow other deep branches vertebrate...
Pituitary adenylate cyclase-activating polypeptides (PACAP-27 and PACAP-38) are neuropeptides of the vasoactive intestinal polypeptide (VIP)/secretin/glucagon family. PACAP receptors expressed in different brain regions, including cerebellum. We used primary culture rat cerebellar granule neurons to study effect PACAP-38 on apoptosis induced by potassium deprivation. demonstrated that increased survival a dose-dependent manner decreasing extent estimated DNA fragmentation. activation...
Pituitary adenylate cyclase-activating polypeptide (PACAP)-27 and PACAP-38 are neuropeptides of the vasoactive intestinal peptide/secretin/glucagon family. We previously described alternative splicing region encoding third intracellular loop PACAP receptor generating six isoforms with differential signal transduction properties (Spengler, D., Waeber, C., Pantaloni, Holsboer, F., Bockaert, J., Seeburg, P. H., Journot, L. (1993) Nature 365, 170-175). In addition, we demonstrated that potencies...
Teleost fishes, thanks to their rapid evolution of sex determination mechanisms, provide remarkable opportunities study the formation chromosomes and mechanisms driving birth new master determining (MSD) genes. However, evolutionary interplay between MSD genes they harbor is rather unexplored. We characterized a male-specific duplicate anti-Müllerian hormone (amh) as gene in Northern Pike (Esox lucius), using genomic expression evidence well by loss-of-function gain-of-function experiments....
The study of sex determination and chromosome organization in nonmodel species has long been technically challenging, but new sequencing methodologies now enable precise high-throughput identification sex-specific genomic sequences. In particular, restriction site-associated DNA (RAD-Seq) is being extensively applied to explore systems many plant animal species. However, software specifically designed search for visualize sex-biased markers using RAD-Seq data lacking. Here, we present...
We previously reported the identification of mZac, a novel mouse zinc finger protein that shared with p53 ability to regulate concomitantly apoptosis and cell cycle progression. describe here isolation, chromosomal localization, functional in vitro characterization its human homolog. hZAC is widely expressed reveals transactivation DNA-binding activity. inhibits tumor growth through induction apoptotic death G 1 arrest. Thus hZAC, like counterpart, displays antiproliferative properties...
The circadian clock located in the suprachiasmatic nucleus (SCN) organizes autonomic and behavioral rhythms into a near 24 hr time that is adjusted daily to solar cycle via direct projection from retina, retinohypothalamic tract (RHT). This neuronal pathway costores neurotransmitters PACAP glutamate, which seem be important for light-induced resetting of clock. At molecular level genes mPer1 mPer2 are believed target light signaling In this study, we investigated possible role PACAP-type 1...
Background Assisted Reproductive Technologies (ART) are increasingly used in humans; however, their impact is now questioned. At blastocyst stage, the trophectoderm directly contact with an artificial medium environment, which can placental development. This study was designed to carry out in-depth analysis of transcriptome after ART mice. Methodology/Principal Findings Blastocysts were transferred either (1) vivo fertilization and development (control group) or (2) vitro embryo culture....
Genomic imprinting is an epigenetic mechanism that restrains the expression of ∼100 eutherian genes in a parent-of-origin-specific manner. The reason for this selective targeting with seemingly disparate molecular functions unclear. In present work, we show imprinted are coexpressed network regulated at transition from proliferation to quiescence and differentiation during fibroblast cell cycle withdrawal, adipogenesis vitro, muscle regeneration vivo. Imprinted gene regulation not linked...
Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal (VIP) are hypothalamic factors believed to play a role in the regulation of anterior pituitary cell function. However, little is known about expression PACAP/VIP receptor (PVR) subtypes such cells. Three PVR have recently been cloned: PACAP-selective PVR1, PVR2 PVR3, which exhibit similar affinities for PACAP VIP. In present study we used reverse transcription-polymerase chain reaction with PVR-specific...
Genomic imprinting regulates the expression of a group genes monoallelically expressed in parent-of-origin specific manner. Allele-specific DNA methylation occurs at differentially methylated regions (DMRs) these genes. We have previously shown that vitro fertilization and embryo culture result defects imprinted H19-Igf2 locus blastocyst stage. The current study was designed to evaluate consequences manipulations on genomic after implantation mouse. Blastocysts were produced following three...
Abstract Whole‐genome duplications (WGDs) are important evolutionary events. Our understanding of underlying mechanisms, including the evolution duplicated genes after WGD, however, remains incomplete. Teleost fish experienced a common WGD (teleost‐specific genome duplication, or TGD) followed by dramatic adaptive radiation leading to more than half all vertebrate species. The analysis gene expression patterns following TGD at level has been limited lack suitable genomic resources. recent...
PLAGL1/ZAC1 undergoes parental genomic imprinting, is paternally expressed, and a member of the imprinted gene network (IGN). It encodes zinc finger transcription factor with anti-proliferative activity candidate tumor suppressor on 6q24 whose expression frequently lost in various neoplasms. Conversely, gain PLAGL1 function responsible for transient neonatal diabetes mellitus, rare genetic disease that results from defective pancreas development. In present work, we showed Plagl1...
The understanding of the evolution variable sex determination mechanisms across taxa requires comparative studies among closely related species. Following fate a known master sex-determining gene, we traced in an entire teleost order (Esociformes). We discovered that northern pike ( Esox lucius ) gene originated from 65 to 90 million-year-old duplication event and it remained linked on undifferentiated chromosomes for at least 56 million years multiple identified several independent species-...