A5049935568- Assisted Reproductive Technology and Twin Pregnancy
- Prenatal Screening and Diagnostics
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Genetic Syndromes and Imprinting
- Sperm and Testicular Function
- RNA modifications and cancer
- Pregnancy and preeclampsia studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Sexual Differentiation and Disorders
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Ectopic Pregnancy Diagnosis and Management
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Uterine Myomas and Treatments
- Birth, Development, and Health
- Testicular diseases and treatments
- Biomedical Ethics and Regulation
Inserm
2013-2025
Université Paris Cité
2006-2025
Assistance Publique – Hôpitaux de Paris
2006-2025
Groupe Hospitalier Cochin - Port-Royal, Hôtel-Dieu, Broca - La Collégiale
2009-2025
Sorbonne Paris Cité
2024-2025
Université Bourgogne Franche-Comté
2017-2024
Université de Bourgogne
2015-2024
CHU Dijon Bourgogne
2011-2023
Centre de recherche Translationnelle en Médecine moléculaire
2017-2023
Maison des Sciences de l’Homme de Dijon
2015-2019
Abstract Background In the last few years, an increase in imprinting anomalies has been reported children born from Assisted Reproductive Technology (ART). Various clinical and experimental studies also suggest alterations of embryo development after ART. Therefore, there is a need for studying early epigenetic which could result ART manipulations, especially on single embryos. this study, we evaluated impact superovulation, vitro fertilization (IVF) culture conditions proper genomic...
Background To the best of our knowledge, no study has exhaustively evaluated association between maternal morbidities and Coronavirus Disease 2019 (COVID-19) during first wave pandemic in pregnant women. We investigated, natural conceptions assisted reproductive technique (ART) pregnancies, whether were more frequent women with COVID-19 diagnosis compared to without pandemic. Methods findings conducted a retrospective analysis prospectively collected data national cohort all hospitalizations...
Background Assisted Reproductive Technologies (ART) are increasingly used in humans; however, their impact is now questioned. At blastocyst stage, the trophectoderm directly contact with an artificial medium environment, which can placental development. This study was designed to carry out in-depth analysis of transcriptome after ART mice. Methodology/Principal Findings Blastocysts were transferred either (1) vivo fertilization and development (control group) or (2) vitro embryo culture....
Do assisted reproductive technologies alter DNA methylation and/or transcription of transposable elements and imprinted genes in cord blood placenta?After ART, changes some were found placenta samples while modifications for also discovered blood.Recent studies have confirmed the increased risk placenta-related adverse pregnancy outcomes excess disorders with abnormal patterns after which raises issue a potential ART-induced epigenetic risk.A total 51 IVF/ICSI (15 conventional 36 ICSI)...
Many loci maintain parent-of-origin DNA methylation only briefly after fertilization during mammalian development: Whether this form of transient genomic imprinting can impact the early embryonic transcriptome or even have life-long consequences on genome regulation and possibly phenotypes is currently unknown. Here, we report a maternal germline differentially methylated region (DMR) at mouse Gpr1/Zdbf2 (DBF-type zinc finger-containing protein 2) locus, which controls paternal-specific...
Progress in assisted reproductive technologies strongly relies on understanding the regulation of dialogue between oocyte and cumulus cells (CCs). Little is known about role long non-coding RNAs (lncRNAs) human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that abundant metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, LINC01118) CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, VIM-AS1). These validated by...
Cytosine DNA methylation is a heritable epigenetic mark present in most eukaryotic groups. While the patterns and functions of have been extensively studied mouse human, their conservation other vertebrates remains poorly explored. In this study, we interrogated distribution function primary fibroblasts seven vertebrate species including bio-medical models livestock (human, mouse, rabbit, dog, cow, pig, chicken).Our data highlight both divergence functions. We show that chicken genome...
Cancer is a leading cause of death among children worldwide. Treatments used for medically assisted reproduction (MAR) are suspected risk factors because their potential epigenetic disturbance and associated congenital malformations.
Genomic imprinting regulates the expression of a group genes monoallelically expressed in parent-of-origin specific manner. Allele-specific DNA methylation occurs at differentially methylated regions (DMRs) these genes. We have previously shown that vitro fertilization and embryo culture result defects imprinted H19-Igf2 locus blastocyst stage. The current study was designed to evaluate consequences manipulations on genomic after implantation mouse. Blastocysts were produced following three...
Can time-lapse imaging systems make it possible to identify novel early non-invasive biomarkers predict live birth?From mostly high-grade embryos, out of 35 morphometric, morphologic and morphokinetic variables, only pronuclei (PN) position at time PN juxtaposition the absence multinucleated blastomeres 2-cell stage (MNB2cell), were potentially associated with birth.Previous studies indicate that some kinetic markers may be predictive blastocyst development embryonic implantation. Certain...
In animal studies, extensive data revealed the influence of culture medium on embryonic development, foetal growth and behaviour offspring. However, this impact has never been investigated in humans. For first time, we depth effects embryo media health, development infants conceived by Vitro Fertilization until age 5 years old. This single-centre cohort study was based an earlier randomized study. During six months, vitro fertilization attempts (No. 371) were according to two (Single Step...
In couples who have chosen and confirmed the fate of surplus frozen embryos, which factors influence their decision, with a special emphasis on symbolic representation embryo(s)? Embryo gamete donation use significantly cryopreserved embryos. Previous studies report difficulties for to decide whether or not continue storing embryo(s) different been already highlighted including embryo conceptualization, information support provided by medical institution, quality life events. Little is...
Genome-wide studies have begun to link subtle variations in both allelic DNA methylation and parent-of-origin genetic effects with early development. Numerous reports highlighted that the placenta plays a critical role coordinating fetal growth, many key functions regulated by genomic imprinting. With recent description of wide-spread polymorphic placenta-specific imprinting, molecular mechanisms leading this curious epigenetic phenomenon is unknown, as their involvement pregnancies...