A5051212502- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Skin Protection and Aging
- MicroRNA in disease regulation
- RNA Research and Splicing
- Renal and related cancers
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Mesenchymal stem cell research
- Biomedical Ethics and Regulation
- Animal Genetics and Reproduction
- melanin and skin pigmentation
- Nuclear Structure and Function
- DNA and Nucleic Acid Chemistry
- 3D Printing in Biomedical Research
- Cancer therapeutics and mechanisms
- Aging, Elder Care, and Social Issues
- Molecular Biology Techniques and Applications
- Neuroscience and Neural Engineering
Université de Montpellier
2015-2024
Inserm
2014-2024
Institute for Regenerative Medicine & Biotherapy
2013-2024
Hôpital Saint Eloi
2013-2024
Centre Hospitalier Universitaire de Montpellier
2017-2023
Centre National de la Recherche Scientifique
2001-2022
Sorbonne Université
2022
Institut de Biologie Paris-Seine
2022
Institut des Cellules Souches pour le Traitement et l'Étude des Maladies Monogéniques
2017
Université Paris-Sud
2017
Direct reprogramming of somatic cells into induced pluripotent stem (iPSCs) provides a unique opportunity to derive patient-specific with potential applications in tissue replacement therapies and without the ethical concerns human embryonic (hESCs). However, cellular senescence, which contributes aging restricted longevity, has been described as barrier derivation iPSCs. Here we demonstrate, using an optimized protocol, that senescence is not limit age-related physiology reversible. Thus,...
To understand the role of extensive senescence-associated 3D genome reorganization, we generated genome-wide chromatin interaction maps, epigenome, replication-timing, whole-genome bisulfite sequencing, and gene expression profiles from cells entering replicative senescence (RS) or upon oncogene-induced (OIS). We identify heterochromatin domains (SAHDs). Differential intra- versus inter-SAHD interactions lead to formation foci (SAHFs) in OIS but not RS. This OIS-specific configuration brings...
The recent Zika virus (ZIKV) epidemic has highlighted the poor knowledge on its physiopathology. Recent studies showed that ZIKV of Asian lineage, responsible for this international outbreak, causes neuropathology in vitro and vivo. However, two African lineages exist is currently found circulating Africa. original strain was also suggested to be neurovirulent but laboratory usage been criticized due multiple passages. In study, we compared French Polynesian (Asian) an isolated Central...
Abstract Recent advances in cell reprogramming showed that OSKM induction is able to improve physiology vitro and vivo. Here, we show a single short sufficient prevent musculoskeletal functions deterioration of mice, when applied early life. In addition, old age, treated mice have improved tissue structures kidney, spleen, skin, lung, with an increased lifespan 15% associated organ‐specific differential age‐related DNA methylation signatures rejuvenated by the treatment. Altogether, our...
Simplified culture conditions are essential for large-scale drug screening and medical applications of human pluripotent stem cells (hPSCs). However, hPSCs [ie, embryonic (hESCs), induced (iPSCs) prone to genomic instability, a phenomenon that is highly influenced by the conditions. Enzymatic dissociation, cornerstone hPSC systems, has been reported be deleterious, but extent timeline alterations this passaging technique still unclear. We prospectively monitored three hESC lines were...
Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements - it therefore important to determine if they favor outgrowth different subpopulations thereby impact on the heterogeneous composition MSCs. We isolated expanded bone marrow-derived MSCs in parallel HPL FCS significantly increases leads dramatic...
Progress in assisted reproductive technologies strongly relies on understanding the regulation of dialogue between oocyte and cumulus cells (CCs). Little is known about role long non-coding RNAs (lncRNAs) human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that abundant metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, LINC01118) CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, VIM-AS1). These validated by...
Abstract Senescence and epigenetic alterations are two important hallmarks of cellular aging. During aging, cells subjected to stress undergo many cycles damage repair before finally entering either apoptosis or senescence, a permanent state cell cycle arrest. The first biomarkers senescence be identified were increased ß‐galactosidase activity induction p16 INK4a . Another feature senescent is the senescence‐associated secretory phenotype (SASP), complex secretome containing more than 80...
Intrinsic and extrinsic factors, including lifestyle sun exposure, can contribute to cell senescence, which impairs skin homeostasis, that may in turn lead aging. Senescent cells have a specific secretome, called the senescence-associated secretory phenotype (SASP) includes MMPs, CXCLs S100A8/9. Reducing SASP with senotherapeutics is promising strategy reduce Here we evaluated effect of formula containing niacinamide hyaluronic acid, are known limit senescence We conducted three different...
DNA replication is a tightly regulated process that initiates from multiple origins and leads to the faithful transmission of genetic material. For proper replication, chromatin surrounding needs be remodeled. However, remarkably little known on which epigenetic changes are required allow firing origins. Here, we show histone demethylase KDM5C/JARID1C for at early JARID1C dictates assembly pre-initiation complex, driving binding proteins CDC45 PCNA, through demethylation mark H3K4me3. Fork...
In eukaryotic cells, nucleus-cytoplasm exchanges play an important role in genomic regulation. We have analyzed the localization of four nuclear antigens different growth conditions: two replicative proteins, DNA polymerase alpha and proliferating cell antigen (PCNA), oncogenic regulatory c-Myc c-Fos. A kinetic study subcellular these proteins has been done. cultures which cells were sparse, detected nucleus. When proliferation was stopped by high density culture or serum starvation, left...
Significance We show that the temporal order of replication (replication timing, RT), normally an extremely stable cell type-specific chromosomal property, is altered in cells from two different premature aging (progeroid) diseases. By converting patient to stem and redifferentiating them as a model disease progression, we identified TP63 gene one earliest RT alterations was associated with abnormal expression. mutations have been linked other diseases share clinical features progeroid...
In the last decade, number of emerging Flaviviruses described worldwide has increased considerably. Among them Zika virus (ZIKV) and Usutu (USUV) are African mosquito-borne viruses that recently emerged. Recently, ZIKV been intensely studied due to major outbreaks associated with neonatal death birth defects, as well neurological symptoms. USUV pathogenesis remains largely unexplored, despite significant human veterinary disorders. Circulation in Africa was documented more than 50 years ago,...
During Xenopus laevis early development, the genome is replicated in less than 15 min every 30 min. We show that during this period, DNA replication proceeds an atypical manner. Chromosomes become surrounded by a nuclear membrane lamina forming micronuclei or karyomeres. This genomic organization permits prereplication centers gather on condensed chromosomes anaphase and initiates autonomously karyomeres at telophase before reconstruction mitosis completion. The formation of not dependent...