A5080829078- Advancements in Transdermal Drug Delivery
- Dermatology and Skin Diseases
- Skin Protection and Aging
- Bee Products Chemical Analysis
- Contact Dermatitis and Allergies
- Allergic Rhinitis and Sensitization
- Acne and Rosacea Treatments and Effects
- Pain Mechanisms and Treatments
- Essential Oils and Antimicrobial Activity
- Pharmacological Effects of Natural Compounds
- Wound Healing and Treatments
- melanin and skin pigmentation
- Nail Diseases and Treatments
- Animal testing and alternatives
- Effects and risks of endocrine disrupting chemicals
- Therapeutic Uses of Natural Elements
- Neuropeptides and Animal Physiology
- Pain Management and Opioid Use
- Hair Growth and Disorders
- Skin and Cellular Biology Research
- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Spectroscopy Techniques in Biomedical and Chemical Research
- Analytical Chemistry and Chromatography
- Nerve injury and regeneration
Pierre Fabre (France)
2015-2024
Pierre Fabre (Germany)
2012-2023
New York Academy of Sciences
2017
Charité - Universitätsmedizin Berlin
2017
John Wiley & Sons (United States)
2017
Hudson Institute
2017
Hôpital Rangueil
2000-2004
Université Toulouse III - Paul Sabatier
1997-2000
Centre National de la Recherche Scientifique
1994-1995
Skin hydration plays an important role in the optimal physical properties and physiological functions of skin. Despite advancements last decade, dry skin remains most common characteristic human disorders. Thus, it is to understand effect on Stratum Corneum (SC) components. In this respect, our interest consists correlating variations unbound bound water content SC with structural organizational changes lipids proteins using a non-invasive technique: Raman spectroscopy. spectra were acquired...
Abstract The transmembrane protein claudin-1 is a major component of epidermal tight junctions (TJs), which create dynamic paracellular barrier in the epidermis. Claudin-1 downregulation has been linked to atopic dermatitis (AD) pathogenesis but variable levels have also observed healthy skin. To elucidate impact different and diseased skin we determined AD patients controls correlated them TJ function. We strikingly broad range with stable overall function non-lesional However, significant...
The human skin microbiota is quantitatively dominated by Gram-positive bacteria, detected both culture and metagenomics. However, metagenomics revealed a huge variety of Gram-negative taxa generally considered from environmental origin. For species affiliation bacteria in microbiota, clones 16S rRNA gene colonies growing on diverse media were analyzed. Species-level identification was achieved for 81% colonies. Fifty distributed 26 genera identified culture, mostly belonging to...
Abstract OECD test guideline 428 compliant protocol using human skin was used to the penetration of 56 cosmetic‐relevant chemicals. The finite doses (10 μL/cm 2 ) chemicals measured over 24 hours. dermal delivery (DD) (amount in epidermis, dermis and receptor fluid [RF]) ranged between 0.03 ± 0.02 72.61 8.89 μg/cm . DD seven comparable with vivo values. mainly accounted for by amount RF, although there were some exceptions, particularly low While variability due cell outliers donor...
A standard protocol was used to determine partition (K) and diffusion (D) coefficients in dermatomed human skin isolated layers for 50 compounds relevant cosmetics ingredients. K values were measured skin, dermis, whole epidermis, intact stratum corneum (SC), delipidized SC lipids by direct measurements of the radioactivity tissue layers/lipid component vs. buffer samples. D determinations made epidermis using a non-linear regression cumulative receptor fluid content radiolabeled compound,...
Intrinsic and extrinsic factors, including lifestyle sun exposure, can contribute to cell senescence, which impairs skin homeostasis, that may in turn lead aging. Senescent cells have a specific secretome, called the senescence-associated secretory phenotype (SASP) includes MMPs, CXCLs S100A8/9. Reducing SASP with senotherapeutics is promising strategy reduce Here we evaluated effect of formula containing niacinamide hyaluronic acid, are known limit senescence We conducted three different...
Inorganic ultraviolet filters such as titanium dioxide (TiO(2)), safe to use on healthy skin, are often applied compromised and irradiated skin. The aim of this study was evaluate in vitro the cutaneous penetration TiO(2) nanoparticles (≥20 nm primary size), included a sunscreen, intact, damaged, irradiated, damaged/irradiated pigskin. Cutaneous localization after 24-h sunscreen application were investigated quantitatively using inductively coupled plasma-mass spectrometry, qualitatively...
Atopic dermatitis (AD) is a common skin inflammatory disease characterized by the production of thymic stromal lymphopoietin (TSLP) and marked TH 2 polarization. Recent studies suggest that IL-1β contributes to development AD inflammation. Here, we have investigated impact signalling on epidermal homeostasis both healthy subjects patients [with functional filaggrin (FLG) alleles], with particular attention TSLP keratinocyte differentiation. In reconstructed human epidermis (RHE), promoted...
A number of pre-clinical studies have demonstrated the value adrenal medullary allografts in management chronic pain. The present longitudinal survey studied 15 patients transplanted for intractable cancer pain after failure systemic opioids due to persistence undesirable side-effects. Before inclusion, all had their controlled by daily intrathecal (I-Th) morphine administration. main evaluation criteria analgesic activity chromaffin cell allograft was complementary requirement analgesics...
Proper hydration of the stratum corneum (SC) is important for maintaining skin's vital functions. Water loss causes development drying stresses, which can be perceived as ‘tightness’, and plays an role in dry skin damage processes. However, molecular structure modifications arising from water subsequent stress has not been established. We investigated mechanism by studying, ex vivo , behaviors SC components during desorption initially fully hydrated samples using Raman spectroscopy....
Abstract Bacterial infections (e.g., with Staphylococcus aureus ) are serious problems in skin a compromised barrier, such as patients atopic dermatitis. Previously, it was shown that tight junction (TJ) proteins influenced by staphylococcal infection, and TJ function is impaired after infection of the keratinocyte cell line HaCaT. However, functional studies cells or models more similar to human missing. Therefore, we investigated bacterial colonialization live S. primary keratinocytes...
The ADME TF aims to develop in silico skin penetration models using vitro human data for 25 chemicals solubilized wataer. Since there are widely differing opinions on different models, we evaluated 3 open source (DermWin™, CDC and the University of Surrey models) commercial (DSkin, SimCyp TCAT). Simulation cutaneous distribution used a finite dose exposure scenario. None adequately predicted amount chemical that evaporated. This was important since prediction dermal delivery (DD) improved...
Acne is a multifactorial inflammatory disease of the pilosebaceous unit in which Cutibacterium acnes one main triggers. A strong predominance C. phylotype IA1 present acne skin with higher biofilm organization and virulence, promoting local immuno-inflammation, especially Th17 pathway.We evaluated single combined pharmacological properties plant extracts, Myrtus communis (Myrtacine®) Celastrol enriched cell culture (CEE) extracts on acnes/Th17 pathway.The effect Myrtacine® virulence was...
The mechanical properties of the Stratum Corneum (SC) have been studied by different authors at macroscopic level, but modification its ultra structure during extension remains unknown. Moreover, little is described about effect stress on SC barrier function. In this study, we examined changes, molecular uniaxial tensile experiments. This was performed isolated samples using Raman spectroscopy. We could identify strain status analyzed combination spectra and Partial Least Squares processing....
Abstract Partition (K) and diffusion (D) coefficients are important to measure for the modelling of skin penetration chemicals through stratum corneum (SC). We compared feasibility three protocols testing 50 in our main studies, using cosmetics‐relevant model with a wide range logP values. Protocol 1: SC concentration‐depth profile tape‐stripping (measures K SC/v D /H 2 , where H is thickness); 2A: incubation isolated chemical (direct measurement only) 2B: mounted on Franz cell based Fick's...
Synopsis Objective Outer root sheath ( ORS ) cells of human hair follicles are a readily available, non‐invasive source keratinocytes for epidermis reconstruction. The aim this study was to characterize model reconstructed from ‐derived model) and evaluate its reproducibility, in comparison with native skin two marketed models (model A, Episkin ® B, Skinethic ). Methods Cell morphology tissue architecture the three were analysed histologically proliferation differentiation marker expression...
<b><i>Background:</i></b> The Cosmetics Europe ADME Task Force is developing in vitro and silico tools for predicting skin systemic concentrations after topical application of cosmetic ingredients. There are conflicting reports as to whether the freezing process affects penetration chemicals; therefore, we evaluated storage human used our studies (8-12 weeks at -20°C) affected model chemicals. <b><i>Methods:</i></b> Finite doses...