A5043570716- Skin Protection and Aging
- Telomeres, Telomerase, and Senescence
- DNA Repair Mechanisms
- Hepatitis C virus research
- melanin and skin pigmentation
- Extracellular vesicles in disease
- Genomics and Chromatin Dynamics
- Hepatitis B Virus Studies
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Acute Myeloid Leukemia Research
- Cancer Diagnosis and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- DNA and Nucleic Acid Chemistry
- Congenital Heart Disease Studies
- Advanced biosensing and bioanalysis techniques
- Autophagy in Disease and Therapy
- Birth, Development, and Health
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Genetics, Aging, and Longevity in Model Organisms
- Corneal Surgery and Treatments
- MicroRNA in disease regulation
Institute for Regenerative Medicine & Biotherapy
2018-2024
Inserm
2011-2024
Université de Montpellier
2018-2023
Hôpital Saint Eloi
2021-2023
Sorbonne Université
2022
Institut de Biologie Paris-Seine
2022
Institut Bergonié
2014
Genomics (United Kingdom)
2013
Institut Mondor de Recherche Biomédicale
2013
University of Leeds
2013
To understand the role of extensive senescence-associated 3D genome reorganization, we generated genome-wide chromatin interaction maps, epigenome, replication-timing, whole-genome bisulfite sequencing, and gene expression profiles from cells entering replicative senescence (RS) or upon oncogene-induced (OIS). We identify heterochromatin domains (SAHDs). Differential intra- versus inter-SAHD interactions lead to formation foci (SAHFs) in OIS but not RS. This OIS-specific configuration brings...
Abstract Recent advances in cell reprogramming showed that OSKM induction is able to improve physiology vitro and vivo. Here, we show a single short sufficient prevent musculoskeletal functions deterioration of mice, when applied early life. In addition, old age, treated mice have improved tissue structures kidney, spleen, skin, lung, with an increased lifespan 15% associated organ‐specific differential age‐related DNA methylation signatures rejuvenated by the treatment. Altogether, our...
G-quadruplex ligands exert their antiproliferative effects through telomere-dependent and telomere-independent mechanisms, but the inter-relationships among autophagy, cell growth arrest death induced by these remain largely unexplored. Here, we demonstrate that ligand 20A causes of cancer cells in culture a HeLa xenografted mouse model. This response is associated with induction senescence apoptosis. Transcriptomic analysis treated reveals significant functional enrichment biological...
Intrinsic and extrinsic factors, including lifestyle sun exposure, can contribute to cell senescence, which impairs skin homeostasis, that may in turn lead aging. Senescent cells have a specific secretome, called the senescence-associated secretory phenotype (SASP) includes MMPs, CXCLs S100A8/9. Reducing SASP with senotherapeutics is promising strategy reduce Here we evaluated effect of formula containing niacinamide hyaluronic acid, are known limit senescence We conducted three different...
Hepatitis B virus (HBV) resistance to nucleoside/nucleotide analogs is frequent. Ultra-deep pyrosequencing (UDPS) a powerful new tool that can detect minor viral variants and characterize complex quasispecies mixtures. We used UDPS analyze the dynamics of adefovir-resistant HBV in patients with chronic infection whom adefovir occurred during treatment. Amino acid substitutions known confer were detected at baseline most patients. The differed among as result evolving differences variant...
Organismal aging entails a gradual decline of normal physiological functions and major contributor to this is withdrawal the cell cycle, known as senescence. Senescence can result from telomere diminution leading finite number population doublings, replicative senescence (RS), or oncogene overexpression, protective mechanism against cancer. associated with large-scale chromatin re-organization changes in gene expression. Replication stress complex phenomenon, defined slowing stalling...
Abstract Forced and maintained expression of four transcription factors OCT4, SOX2, KLF4 c-MYC (OSKM), can reprogram somatic cells into induced Pluripotent Stem Cells (iPSCs) a limited OSKM induction is able to rejuvenate the cell physiology without changing identity. We therefore sought determine if burst might improve tissue fitness delay age-related pathologies in whole animal. For this, we used sensitive model heterozygous premature aging mice carrying just one mutated Lamin A allele...
ABSTRACT Research and financial efforts spent on biodefense technologies highlight the current concern for biothreat event preparedness. Nonhazardous but relevant “simulant” microorganisms are typically used to simplify technological developments, testing, staff training. The bacteriophage MS2, a small RNA virus, is classically as reference simulant viruses within community. However, variola considered major threat, displays very different features (size, envelope, double-stranded DNA...
Abstract Objective Skin ageing is linked to the accumulation of senescent cells and a “senescence‐associated secretory phenotype” (SASP). SASP factors include chemokines, cytokines, small extracellular vesicles (EVs) containing miRNAs. We characterized profile markers in normal human dermal fibroblasts (HDFs) evaluated effect Haritaki fruit extract on these senescence markers. Methods Senescence was induced HDFs by ionizing radiation (X ray), followed 14 days culture. Parallel incubations...
Abstract The origins of congenital heart diseases, the most common diseases are still largely unknown. Environmental factors now emerging as major causes these diseases. Vitamin D deficiency has become a public health burden, notably for childbearing age, pregnant and breastfeeding women. Since maternal 25-hydroxyvitamin (25(OH)D) determined fetal neonatal 25(OH)D status, foetuses exposed to insufficient levels vitamin D, may feature developmental defects. Herein, we investigated effects on...
Backgrounds: Cellular senescence (CS) is a response to diverse forms of nonlethal cellular stress.The phenotypic transformations occurring in CS include stable cell cycle arrest, an inflammatory response, and complex metabolic shift.Among the most prominent intrinsic stimuli are genotoxic oncogenic perturbations, many which can initiate or promote aging age-related diseases.Thus, accumulation senescent cells tissues emerges as critical driver aging.Consistently, be found affected patients...