A5046361428- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Cancer, Hypoxia, and Metabolism
- Thyroid Disorders and Treatments
- Fatty Acid Research and Health
- Peroxisome Proliferator-Activated Receptors
- RNA Research and Splicing
- Sirtuins and Resveratrol in Medicine
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- RNA modifications and cancer
- Muscle metabolism and nutrition
- Hearing, Cochlea, Tinnitus, Genetics
- ATP Synthase and ATPases Research
- Pancreatic function and diabetes
- Retinoids in leukemia and cellular processes
- Exercise and Physiological Responses
- Diet, Metabolism, and Disease
- Lipid metabolism and biosynthesis
- interferon and immune responses
- Cancer-related gene regulation
- Autophagy in Disease and Therapy
- Metabolism and Genetic Disorders
- Metabolism, Diabetes, and Cancer
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Dynamique Musculaire et Métabolisme
2015-2024
Université de Montpellier
2013-2023
Inserm
2022
Institut Agro Montpellier
2005-2021
Institut National de la Recherche Agronomique
2001-2019
Cambridge University Press
2010
New York University Press
2010
Immunology from Concept and Experiments to Translation
2008-2009
École nationale supérieure d'arts et métiers
2001-2006
In earlier research, we identified a 43-kDa c-ErbAα1 protein (p43) in the mitochondrial matrix of rat liver. present work, binding experiments indicate that p43 displays an affinity for triiodothyronine (T3) similar to T3 nuclear receptor. Using organello import experiments, found is targeted organelle by unusual process previously reported MTF1, yeast transcription factor. DNA-binding demonstrated specifically binds four DNA sequences with high similarity response elements (mt-T3REs)....
To characterize the regulatory pathways involved in inhibition of cell differentiation induced by impairment mitochondrial activity, we investigated relationships occurring between organelle activity and myogenesis using an avian myoblast line (QM7). The translation chloramphenicol led to a potent block differentiation. Carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone oligomycin, which affect at different levels, exerted similar influence. In addition, provided evidence that this...
Aims: In our aging society, age-related hearing loss (ARHL) or presbycusis is increasingly important. Here, we study the mechanism of ARHL using senescence-accelerated mouse prone 8 (SAMP8) which a useful model to probe effects on biological processes. Results: We found that SAMP8 strain displays premature and cochlear degeneration recapitulating processes observed in human (i.e., strial, sensory, neural degeneration). The molecular mechanisms associated with mice involve oxidative stress,...
In our aging society, age-related hearing loss (ARHL) has become a major socioeconomic issue. Reactive oxygen species (ROS) may be one of the main causal factors cochlear cell degeneration. We examined whether ROS-induced DNA damage response drives senescence and contributes to ARHL from cellular up system level. Our results revealed that sublethal concentrations hydrogen peroxide (H2O2) exposure initiated illustrated by increased γH2AX 53BP1 expression foci formation mainly in sensory hair...
Abstract Recent advances in cell reprogramming showed that OSKM induction is able to improve physiology vitro and vivo. Here, we show a single short sufficient prevent musculoskeletal functions deterioration of mice, when applied early life. In addition, old age, treated mice have improved tissue structures kidney, spleen, skin, lung, with an increased lifespan 15% associated organ‐specific differential age‐related DNA methylation signatures rejuvenated by the treatment. Altogether, our...
Chicoric acid (CA) is a caffeoyl derivative previously described as having potential anti-diabetic properties. As similarities in cellular mechanism between diabetes and aging have been shown, we explored on L6 myotubes the effect of CA modulation intracellular pathways involved aging. We also determined its influence lifespan Caenorhabditis elegans worm (C. elegans). In myotubes, was potent reactive oxygen species (ROS) scavenger, reducing ROS accumulation under basal well oxidative stress...
Oculopharyngeal muscular dystrophy (OPMD), a late-onset disorder characterized by progressive degeneration of specific muscles, results from the extension polyalanine tract in poly(A) binding protein nuclear 1 (PABPN1). While roles PABPN1 polyadenylation and regulation alternative site choice are established, molecular mechanisms behind OPMD remain undetermined. Here, we show, using Drosophila mouse models, that pathogenesis depends on affected tail lengths mRNAs. We identify set mRNAs...
Natural deep eutectic solvents (NaDES) are new natural in green chemistry that some cases have been shown to allow better extraction of plant bioactive molecules compared conventional and higher phenolic compound absorption rodents. However, there is a serious lack information regarding their vivo safety. The purpose this study was verify the safety NaDES (betaine:glycerol (1:2 mole ratio) water) extract from coffee beans, rich polyphenols. Twelve 6-week-old male Wistar rats were randomized...
Inflammation and oxidative stress are thought to be involved in, or associated with, the development of obesity, dyslipidemia, hepatic steatosis, insulin resistance. This work was designed determine evolution inflammation during onset progression steatosis glucose intolerance. Seventy-five male Wistar rats were divided control high-fat high-fructose (HFHFr) groups. A subgroup each group sacrificed at 4, 8, 12, 16, 20 weeks. HFHFr-fed exhibited overweight, intolerance, with increased contents...
The disruption of the synaptic connection between sensory inner hair cells (IHCs) and auditory nerve fiber terminals type I spiral ganglion neurons (SGN) has been observed early in several pathologies (e.g., noise-induced or ototoxic drug-induced age-related hearing loss). It suggested that glutamate excitotoxicity may be an inciting element degenerative cascade these pathological cochlear conditions. Moreover, oxidative damage induced by free hydroxyl radicals nitric oxide dramatically...
Metabolic dysfunction-associated fatty liver disease (MAFLD) is the most common form of chronic disease, for which only resmetirom has recently received FDA approval. Prevention crucial, as it can help manage and potentially reverse progression MAFLD to more severe stages. Omega-3 acids, are a type polyunsaturated acid (PUFA), have numerous beneficial effects in health including disease. Other bioactive lipids, such furanic acids (FuFA) hydroxy esters (FAHFA), also demonstrated several...
The importance of mitochondrial activity has recently been extended to the regulation developmental processes. Numerous pathologies associated with organelle's dysfunctions emphasize their physiological importance. However, genome transcription, a key element for function, remains poorly understood. After characterization in organelle truncated form triiodothyronine nuclear receptor (p43), T3-dependent transcription factor genome, our purpose was search other receptors involved...
Abstract We have previously shown that mitochondrial activity is an important regulator of myoblast differentiation, partly through processes targeting myogenin expression. Here, we investigated the possible involvement c‐myc in these processes. Inhibition by chloramphenicol abrogated decrease mRNA and protein levels occurring at onset terminal differentiation. Conversely, stimulation overexpression T3 receptor (p43) down‐regulated In addition, mimicked influence inhibition on Moreover, like...
In previous studies, we have characterized a new hormonal pathway involving mitochondrial T3 receptor (p43) acting as transcription factor and consequently stimulating activity biogenesis. We established the involvement of this in regulation vitro myoblast differentiation.We generated mice overexpressing p43 under control human α-skeletal actin promoter. agreement with characterization promoter, northern-blot western-blot experiments confirmed that after birth was specifically overexpressed...
In previous studies, we characterized a new hormonal pathway involving mitochondrial T3 receptor (p43) acting as transcription factor. in vitro and vivo have shown that p43 increases biogenesis. addition, overexpression skeletal muscle stimulates respiration induces shift metabolic contractile features of fibers which became more oxidative. Here studied the influence mice during aging. We report initially increased mass. However, after early rise DNA occurring at 2 months age transgenic...
In vertebrates, skeletal muscle myofibers display different contractile and metabolic properties associated with mitochondrial content activity. We have previously identified a triiodothyronine receptor (p43) regulating transcription biogenesis. When overexpressed in muscle, it increases DNA content, stimulates respiration, induces shift the features of fibers toward slower more oxidative phenotype. Here we show that p43 depletion mice decreases replication respiratory chain activity...
One of the most conserved features all cancers is a profound reprogramming cellular metabolism, favoring biosynthetic processes and limiting catalytic processes. With acquired knowledge some these important changes, we have designed combination therapy in order to force cancer cells use particular metabolic pathway that ultimately results accumulation toxic products. This innovative approach consists blocking lipid synthesis, at same time cell, through inhibition AMP-activated kinase,...
Thyroid hormone is a major determinant of energy expenditure and key regulator mitochondrial activity. We have previously identified triiodothyronine receptor (p43) that acts as transcription factor the organelle genome, which leads, in vitro vivo, to stimulation biogenesis. Here we generated mice specifically lacking p43 address its physiological influence. found required for normal glucose homeostasis. The p43−/− had defect insulin secretion both vivo isolated pancreatic islets loss...