A5038763634- Cancer, Lipids, and Metabolism
- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Asthma and respiratory diseases
- Immune Response and Inflammation
- Estrogen and related hormone effects
- Cytokine Signaling Pathways and Interactions
- Protein purification and stability
- Neonatal Respiratory Health Research
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- Transgenic Plants and Applications
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Mast cells and histamine
- Protease and Inhibitor Mechanisms
- Plant-Microbe Interactions and Immunity
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genetics and Neurodevelopmental Disorders
- Receptor Mechanisms and Signaling
- HIV/AIDS drug development and treatment
- Fatty Acid Research and Health
- Protein Kinase Regulation and GTPase Signaling
Inserm
2013-2024
Institut de Recherche en Cancérologie de Montpellier
2015-2024
Université de Montpellier
2010-2024
Centre National de la Recherche Scientifique
2010
Institut Universitaire de Recherche Clinique
2010
Institut des Biomolécules Max Mousseron
2010
Centre Hospitalier Universitaire Henri-Mondor
2010
Gefluc Languedoc Roussillon
2009
Hôpital Arnaud de Villeneuve
1999
Plant nucleotide-binding and leucine-rich repeat domain proteins (NLRs) are immune sensors that recognize pathogen effectors. Here, we show molecular engineering of the integrated decoy (ID) an NLR can extend its recognition spectrum to a new effector. We relied for this on detailed knowledge Magnaporthe oryzae effectors AVR-PikD, AVR-Pia, AVR1-CO39 by, respectively, rice NLRs Pikp-1 RGA5. Both receptors detect their through physical binding HMA (Heavy Metal-Associated) IDs. By introducing...
Abstract Increased de novo fatty acid (FA) synthesis is one hallmark of tumor cells, including prostate cancer. We present here our most recent results showing that lipid composition in human cancer characterized by an increased ratio monounsaturated FA to saturated FA, compared with normal prostate, and evidence the overexpression lipogenic enzyme stearoyl-CoA desaturase 1 (SCD1) As a new therapeutic strategy, we show pharmacologic inhibition SCD1 activity impairs decreased proliferation...
Abstract Background Antidepressants are heavily prescribed drugs and have been shown to affect inflammatory signals. We examined whether these anti-inflammatory properties in animal models of septic shock allergic asthma. also analysed antidepressants act directly on peripheral cell types that participate the response diseases. Methods The desipramine fluoxetine were compared vivo glucocorticoid prednisolone, an drug reference. In a murine model lipopolysaccharides (LPS)-induced shock,...
Micro RNAs are small, non-coding, single-stranded that negatively regulate gene expression at the post-transcriptional level. Since miR-143 was found to be down-regulated in prostate cancer cells, we wanted analyze its human cancer, and test ability of miR-43 arrest cell growth vitro vivo.Expression analyzed cancers by quantitative PCR, situ hybridization. introduced cells vivo electroporation. Bioinformatics analysis luciferase-based assays were used determine targets. We show this study...
Hormonal regulation of gene activity is mediated by nuclear receptors acting as ligand-activated transcription factors. Intermediary factors interacting with their activation functions are required to mediate transcriptional stimulation. In search such receptor proteins, we have screened a human cDNA expression library and isolated protein that interacts in vitro transcriptionally active estrogen (ER). Sequence analysis reveals this the homolog mouse TIF1 (transcription intermediary factor...
Airway remodeling is a well-recognized feature in patients with chronic asthma. The accumulation the submucosa of fibrous proteins that are substrates matrix metalloproteinases (MMP), and demonstration increased levels MMP-9 bronchoalveolar lavage fluid, prompted us to determine whether there was an imbalance between MMPs tissue inhibitors metalloproteinase (TIMP) such patients. We investigated presence TIMPs other MMPs. TIMP were compared those all inflammatory cytokines. Adults stable...
One of the most conserved features all cancers is a profound reprogramming cellular metabolism, favoring biosynthetic processes and limiting catalytic processes. With acquired knowledge some these important changes, we have designed combination therapy in order to force cancer cells use particular metabolic pathway that ultimately results accumulation toxic products. This innovative approach consists blocking lipid synthesis, at same time cell, through inhibition AMP-activated kinase,...
Significance The 20S proteasome is a key actor of the control protein levels and integrity in cells. To perform its multiple functions, it works with series regulators, among which nuclear complex called PA28γ. In particular, PA28γ participates regulation cell proliferation dynamics. We describe here characterization protein, PIP30/FAM192A, binds tightly to favors interaction while inhibiting association coilin, central component Cajal bodies. Thus, PIP30/FAM192A critically controls...
Despite decades of clinical and preclinical investigations, we still poorly grasp our innate immune response to human adenoviruses (HAdVs) their vectors. In this study, explored the impact lactoferrin on three HAdV types that are being used as vectors for vaccines. Lactoferrin is a secreted globular glycoprotein influences direct indirect against range pathogens following breach in tissue homeostasis. The mechanism by which complexes increases uptake induce maturation phagocytes unknown. We...
Histamine H1-receptor antagonists are used to relieve the symptoms of an immediate allergic reaction. They have additional anti-inflammatory effects that could result from inhibition transcription factors activator protein-1 (AP-1) and nuclear factor-kappa B (NF-kappaB). The implication in these is controversial. Diphenhydramine a first-generation antagonist while mizolastine desloratadine second-generation compounds. Mizolastine also inhibitor 5-lipoxygenase (5-LO), enzyme has been involved...
Antibody-drug conjugates, such as brentuximab vedotin (BTXv), are an innovative category of monoclonal antibodies. BTXv is bioconjugated via the chemical reduction cysteine residues involved in disulfide bonds. Species containing zero, two, four, six, or eight molecules per antibody coexist stock solution. We investigated influence drug loading on binding to FcRn, a major determinant pharmacokinetics humans. developed hydrophobic interaction chromatography (HIC) method for separating...
Recombinant antibody single-chain variable fragments (scFv) are difficult to purify homogeneously from a protein complex mixture. The most effective, specific and fastest method of purification is an affinity chromatography on Protein L (PpL) matrix. This multi-domain bacterial surface that able interact with conformational patterns kappa light chains. It mainly recognizes amino acid residues located at the VL FR1 some in constant (CL) domain. Not all chains recognized, however, lack CL can...
By virtual screening using a fragment-based drug design (FBDD) approach, 33 fragments were selected within small pockets around interaction hot spots on the Sec7 surface of nucleotide exchange factor Arno, and then their ability to interfere with Arno-catalyzed G-protein Arf1 was evaluated. use SPR, NMR, fluorescence assays, direct binding three identified Arno domain demonstrated promiscuous aggregate behavior Then mode one fragment more active analogue solved by X-ray crystallography. This...
Club cell secretory protein (CCSP) knockout mice exhibit increased airway neutrophilia, as found in chronic obstructive pulmonary disease (COPD). We therefore investigated whether treating COPD epithelia with recombinant human CCSP (rhCCSP) could dampen exaggerated neutrophilia.Control, smoker and air-liquid interface (ALI) cultures exposed to cigarette smoke extract (CSE) were treated without rhCCSP. The chemotactic properties of the supernatants assessed using Dunn chambers. Neutrophil...
SUMMARY Plant nucleotide-binding and leucine-rich repeat domain proteins (NLRs) are immune sensors that specifically recognize pathogen effectors induce responses. Designing artificial NLRs with new effector recognition specificities is a promising prospect for sustainable, knowledge-driven crop protection. However, such strategies hampered by the complexity of NLR function. Here, we tested whether molecular engineering integrated decoy (ID) an could extend its spectrum to effector. To this...
Bone morphogenetic proteins (BMPs) regulate diverse cellular responses during embryogenesis and in adulthood including cell differentiation, proliferation, death various tissues. In the adult pituitary, BMPs participate control of hormone secretion suggesting a potential endocrine/paracrine role for BMPs, but some mechanisms are unclear. Here, using bioactivity test based on embryonic cells (C3H10T1/2) transfected with BMP-responsive element, we sought to determine whether pituitary secrete...
Summary Background Glucocorticoids exert their anti‐inflammatory effects mainly through transrepression of the transcription factors activator protein‐1 (AP‐1) and nuclear factor‐kappa B (NF‐κB). Certain adverse glucocorticoids are mediated gene transactivation. Fluticasone propionate (FP) mometasone furoate (MF) most recently developed topical for treatment airway disorders. Their relative capacities to repress AP‐1 NF‐κB activities not known comparison transactivation potencies has given...