A5062385342- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- Fungal and yeast genetics research
- Cellular transport and secretion
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Photosynthetic Processes and Mechanisms
- Protein Kinase Regulation and GTPase Signaling
- Pancreatic function and diabetes
- 14-3-3 protein interactions
- Genetics and Neurodevelopmental Disorders
- Endoplasmic Reticulum Stress and Disease
- Polyamine Metabolism and Applications
- Cancer, Hypoxia, and Metabolism
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Signaling Pathways in Disease
- Protein Tyrosine Phosphatases
- Reproductive Biology and Fertility
- Plant nutrient uptake and metabolism
- Advanced NMR Techniques and Applications
- Liver physiology and pathology
Centre National de la Recherche Scientifique
2004-2024
Université de Montpellier
2007-2024
Centre de Recherche en Biologie cellulaire de Montpellier
1997-2021
Medical Research Council
1999-2000
National Institute for Medical Research
1999
Inserm
1991-1996
University of Birmingham
1993
Institut de Recherche en Infectiologie de Montpellier
1992
Observatoire Océanologique de Banyuls-sur-Mer
1991
ABSTRACT Dbf2 is a multifunctional protein kinase in Saccharomyces cerevisiae that functions transcription, the stress response and as part of network genes exit from mitosis. By analogy with fission yeast it seemed likely these mitotic would be involved cytokinesis. As preliminary investigation this we have used tagged GFP to examine intracellular localisation living cells. found on centrosomes/ spindle pole bodies (SPBs) also at bud neck where forms double ring. The cell cycle regulated....
We have produced human cyclin A in Escherichia coli and investigated how it generates H1 kistone kinase activity when added to cyclin-free extracts prepared from parthenogenetically activated Xenopus eggs. Cyclin was found form a major complex with cdc2, bind cdk2/Eg1 only poorly. No lag phase detected between the time histone frog extracts, even presence of 2 mM vanadate, which blocks cdc25 activity. Essentially identical results were obtained using starfish oocytes. conclude that formation...
In eukaryotes an abnormal spindle activates a conserved checkpoint consisting of the MAD and BUB genes that results in mitotic arrest at metaphase. Recently, we others identified novel Bub2-dependent branch to this blocks exit. This cell-cycle depends upon inhibition G-protein Tem1 appears be regulated by Bfa1/Bub2, two-component GTPase-activating protein, exchange factor Lte1. Here, find Bub2 Bfa1 physically associate across entire cell cycle bind during mitosis early G1. is multiply...
Inhibition of okadaic acid-sensitive phosphatases released the cyclin degradation pathway from its inhibited state in extracts prepared unfertilized Xenopus eggs arrested at second meiotic metaphase. It also switched on protease activity a permanent fashion interphase activated eggs. Even after cdc2 kinase inactivation, microinjection acid-treated pushed G2-arrested recipient oocytes into M phase, suggesting that phosphatase inhibitor stabilizes an unidentified factor which shares common...
Significance The 20S proteasome is a key actor of the control protein levels and integrity in cells. To perform its multiple functions, it works with series regulators, among which nuclear complex called PA28γ. In particular, PA28γ participates regulation cell proliferation dynamics. We describe here characterization protein, PIP30/FAM192A, binds tightly to favors interaction while inhibiting association coilin, central component Cajal bodies. Thus, PIP30/FAM192A critically controls...
Polyglutamylation is a post-translational modification initially discovered on tubulin. It has been implicated in multiple microtubule functions, including neuronal differentiation, axonemal beating and stability of the centrioles, shown to modulate interaction between tubulin associated proteins. The enzymes catalysing this are not yet known. Starting with partially purified fraction mouse brain polyglutamylase, monoclonal antibodies were raised used further purify enzyme by...
The completion of cytokinesis requires abscission the midbody, a microtubule-rich cytoplasmic bridge that connects daughter cells before their final separation. Although it has been established both midbody structure and membrane fusion are essential for abscission, biochemical machinery cellular processes remain ill-defined. Here we report human Mob1A Mob1B proteins involved in regulation intercellular bridge. Mob family is group highly conserved eukaryotes, described as binding partners...
The formation of cdk-cyclin complexes has been investigated at the molecular level and quantified using spectroscopic approaches. In absence phosphorylation, cdk2, cdc2, cdk7 form highly stable with their "natural" cyclin partners dissociation constants in nanomolar range. contrast, nonphosphorylated cdc2-cyclin H, cdk2-cyclin cdk7-cyclin A present a 25-fold lower stability. On basis both structure complex on our kinetic results, we suggest that interaction any cdk involves same hydrophobic...
Purified cyclin B-cdc2 kinase has been shown previously to trigger degradation in interphase frog extracts by initiating a cascade of reactions that includes ubiquitinylation and ends with proteolysis. However, A-cdc2 was not assayed these early experiments. Here we have full-length recombinant human A failed induce when it added free B, although formed an active complex Xenopus cdc2. highly purified containing truncated starfish cdc2 also switch on the pathway. In contrast, both B readily...
Truncated cyclin A and B lacking the N-terminal domain comprising 'destruction box' escape from proteolysis arrest cells at metaphase. Mutation of a conserved arginine residue destruction makes resistant to proteolysis. Here we show that mutation same also proteolysis, in either two situations which degradation pathway is turned on: (i) Xenopus extracts activated eggs where has been permanently on by adding recombinant undegradable box substituted alanine; (ii) metaphase II-arrested oocytes...