A5025876236- Microtubule and mitosis dynamics
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Cardiac electrophysiology and arrhythmias
- Cancer-related Molecular Pathways
- Sperm and Testicular Function
- Lipid metabolism and disorders
- Osteoarthritis Treatment and Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Geological and Geochemical Analysis
- Ion channel regulation and function
- Reproductive Physiology in Livestock
- Developmental Biology and Gene Regulation
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Radiopharmaceutical Chemistry and Applications
- HIV/AIDS drug development and treatment
- Genomics and Chromatin Dynamics
- Protein Kinase Regulation and GTPase Signaling
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
Centre National de la Recherche Scientifique
2008-2024
Université de Montpellier
1994-2024
Centre de Recherche en Biologie cellulaire de Montpellier
2007-2020
McGill University
1997-2020
American Dental Education Association
2020
Charles River Laboratories (Netherlands)
2020
John Wiley & Sons (United States)
2020
Université de Sherbrooke
1986-2018
Jewish General Hospital
1997-2017
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2017
ABSTRACT By differential screening of a Xenopus laevis egg cDNA library, we have isolated 2,111 bp which corresponds to maternal mRNA specifically deadenylated after fertilisation. This cDNA, called Eg2, encodes 407 amino acid protein kinase. The pEg2 sequence shows significant identity with members new kinase sub-family includes Aurora from Drosophila and Ipl1 (increase in ploidy-1) budding yeast, enzymes involved centrosome migration chromosome segregation, respectively. A single 46 kDa...
Collective cell migration (CCM) is essential for organism development, wound healing, and metastatic transition, the primary cause of cancer-related death, it involves cell–cell adhesion molecules cadherin family. Increased P-cadherin expression levels are correlated with tumor aggressiveness in carcinoma aggressive sarcoma; however, how promotes malignancy remains unknown. Here, using integrated biology biophysical approaches, we determined that specifically induces polarization CCM through...
Abstract Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living are bivalves. Yet, the origin of hemocytes, involved innate immunity, unknown bivalves and current knowledge about mollusk adult somatic scarce. Here we identify population precursor their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like...
Research Article| December 01, 1990 Direct dating of stromatolitic carbonates from the Schmidtsdrif Formation (Transvaal Dolomite), South Africa, with implications on age Ventersdorp Supergroup Bor-ming Jahn; Jahn 1Centre Armoricain d'Etude Structurale des Socles-Centre National de la Recherche Scientifique, Université Rennes, 35042 Rennes Cédex, France Search for other works by this author on: GSW Google Scholar J. Bertrand-Sarfati; Bertrand-Sarfati 2Centre Géologique et Géophysique,...
Background— Arrhythmias associated with QT prolongation on the ECG often lead to sudden unexpected death in epilepsy. The mechanism causing a of interval during epilepsy remains unknown. Based observations showing an upregulation neuronal sodium channels brain epilepsy, we tested hypothesis that similar phenomenon occurs heart and contributes by altering cardiac current properties (I Na ). Methods Results— We used patch clamp technique assess effects action potential I rat ventricular...
Upon maturation in the bone marrow, polyploid megakaryocytes elongate very long and thin cytoplasmic branches called proplatelets. Proplatelets enter sinusoids blood vessels which platelets are ultimately released. Microtubule dynamics, bundling, sliding, coiling, drive these dramatic morphological changes whose regulation remains poorly understood. properties defined by tubulin isotype composition post-translational modification patterns. It unknown whether microtubule modifications occur...
Coordination of the different cytoskeleton networks in cell is central importance for morphogenesis, organelle transport, and motility. The Rho family proteins are well characterized their effects on actin cytoskeleton, but increasing evidence indicates that they may also control microtubule (MT) dynamics. Here, we demonstrate a novel Cdc42/Rac effector, X-p21-activated kinase (PAK)5, colocalizes binds to both MT its subcellular localization regulated during cycle progression. In transfected...
The adenovirus DNA-binding protein (DBP) is an abundant multifunctional located primarily in the nuclei of infected cells. To define sequences involved nuclear transport DBP, a series point and small deletion mutants were constructed via oligonucleotide-directed mutagenesis. Two short stretches basic amino acids amino-terminal domain (amino 42 to 46 84 89) identified. Their importance, however, depended on context which DBP was expressed. Disruption either site prevented localization after...
Ran is an essential GTPase that controls nucleocytoplasmic transport, mitosis, and nuclear envelope formation. These functions are regulated by interaction of with different partners, formation a Ran-GTP gradient emanating from chromatin. Here, we identify novel level regulation. We show substrate for p21-activated kinase 4 (PAK4) its phosphorylation on serine-135 increases during mitosis. The endogenous phosphorylated active PAK4 dynamically associate components the microtubule spindle...
// Mohamed Jemaà 1 , Gwenola Manic 2 Gwendaline Lledo Delphine Lissa 3, 4, 5, 6 Christelle Reynes 7 Nathalie Morin Frédéric Chibon 8, 9 Antonella Sistigu Maria Castedo Ilio Vitale 2, 10, * Guido Kroemer 11, 12, 13, Ariane Abrieu 1, CRBM, CNRS UMR5237, Université de Montpellier, France Regina Elena National Cancer Institute, Rome, Italy 3 Paris-Sud/Paris XI, Le Kremlin-Bicêtre, 4 INSERM, UMRS1138, Paris, 5 Equipe 11 Labelisée par la Ligue Nationale Contre le Cancer, Centre Recherche des...
Interacting domains in human immunodeficiency virus type 1 (HIV-1) Gag precursor (Pr55gag) expressed recombinant baculovirus-infected cells were investigated by three different methods: (i) trans rescue and coencapsidation of C-terminal deletion (amber) mutants chimeras into retrovirus-like particles complementation experiments with HIV-1 wild-type (WT) Pr55gag, (ii) Gag-Gag interactions vitro ligand affinity blotting assays, (iii) quantitative immunoelectron microscopy particles, using a...
ABSTRACT Unfertilized frog eggs arrest at the second meiotic metaphase, due to cytostatic activity of c-mos proto-oncogene (CSF). MAP kinase has been proposed mediate CSF in suppressing cyclin degradation. Using an vitro assay generate activity, and recombinant CL 100 phosphatase inactivate kinase, we confirm that blocks degradation through activation. We further show for suppress degradation, it must be activated before B-cdc2 effectively promoted Thus does not inactivate, but rather...
In this study, Dicentrarchus labrax encephalitis virus (DlEV), which causes sea bass encephalitis, was propagated in cell culture, thus allowing study of its lytic cycle. DlEV infection mammalian and fish cells induced different patterns expression capsid proteins, were assembled as virus-like particles, accumulating the cytoplasm either diffuse masses or vesicles, shown by electron microscopy. These particles correspond to virions, their ability induce secondary infection. Fish proved be...