A5042374393- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Cancer-related Molecular Pathways
- Blood properties and coagulation
- Cell death mechanisms and regulation
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Ion channel regulation and function
- Cancer Genomics and Diagnostics
- Caveolin-1 and cellular processes
- Epigenetics and DNA Methylation
- Neuroblastoma Research and Treatments
- Plant responses to water stress
- Cancer Research and Treatments
- Ion Channels and Receptors
- Venomous Animal Envenomation and Studies
- Sarcoma Diagnosis and Treatment
- Hippo pathway signaling and YAP/TAZ
- Folate and B Vitamins Research
- Cancer Cells and Metastasis
- Neonatal Health and Biochemistry
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Marine Invertebrate Physiology and Ecology
- Chromosomal and Genetic Variations
Tunis El Manar University
2023-2025
Lund University
2017-2024
Centre de Recherche en Biologie cellulaire de Montpellier
2013-2024
Faculté de médecine de Tunis
2023
Digital Research Centre of Sfax
2023
Centre of Biotechnology of Sfax
2014-2022
University of Sfax
2014-2022
Medicon Village
2020-2021
University of Tübingen
2016-2018
Centre National de la Recherche Scientifique
2013-2017
Macroautophagy is known to participate in the quality control and turnover of cytoplasmic organelles, yet there little evidence that macroautophagy targets nuclei mammalian cells. Here, we investigated whether autophagy may target micronuclei, which arise as a result deficient bipolar chromosome segregation cells exposed cell cycle perturbations. After removal several distinct blockers (nocodazole, cytochalasin D, hydroxyurea or SP600125), manifested an increase frequency micronuclei...
SummaryPatients with non-small cell lung cancer (NSCLC) are routinely treated cytotoxic agents such as cisplatin. Through a genome-wide siRNA-based screen, we identified vitamin B6 metabolism central regulator of cisplatin responses in vitro and vivo. By aggravating bioenergetic catastrophe that involves the depletion intracellular glutathione, exacerbates cisplatin-mediated DNA damage, thus sensitizing large panel lines to apoptosis. Moreover, sensitizes cells apoptosis induction by...
Abstract Long-lived animals show a non-observable age-related decline in immune defense, which is provided by blood cells that derive from self-renewing stem cells. The oldest living are bivalves. Yet, the origin of hemocytes, involved innate immunity, unknown bivalves and current knowledge about mollusk adult somatic scarce. Here we identify population precursor their differentiation into hemocytes. Oyster gill contains an as yet unreported irregularly folded structure (IFS) with stem-like...
Significance One hypothetical pathway leading to oncogenic transformation involves a transient phase of tetraploidization, followed by asymmetric cell divisions, aneuploidy, and genomic instability. By means pharmacological screen, we identified resveratrol salicylate as compounds that kill tetraploid cells more efficiently than their parental diploid counterparts. Resveratrol reduced the frequency arising from primary epithelial cultures exposed mitotic inhibitors. In mouse model intestinal...
Tetraploidy is a condition in which the entire set of chromosomes doubles, most often due to errors during cell division. can lead genomic instability and significant consequences, particular metastasis treatment failure tumours, including radiotherapy. The development new strategies sensitise these cells great importance. In our study, we investigated vitro combination chemical with kinase inhibitor SP600125 irradiation on diploid versus metastatic tetraploid RKO colon cancer clones. We...
The bioactive steroid sapogenin diosgenin is considered for a wide variety of applications including treatment malignancy. substance counteracts tumor growth in part by stimulating apoptosis cells. Similar to nucleated cells, erythrocytes may enter suicidal death or eryptosis, which characterized cell shrinkage and membrane scrambling with phosphatidylserine translocation the erythrocyte surface. Signaling involved stimulation eryptosis includes increase cytosolic Ca2+ activity ([Ca2+]i),...
Background . In folk medicine, extra virgin olive oil (EVOO) is used as a remedy for variety of diseases. This study investigates the in vivo antinociceptive, anti-inflammatory, and anti-cancer effects EVOO on mice rats. Materials Methods this experimental study, using acetic acid-induced writhing formalin tests mice, analgesic effect was evaluated. Acetylsalicylic acid morphine were standard drugs, respectively. The anti-inflammatory activity investigated by means carrageenan-induced paw...
// Mohamed Jemaà 1 , Gwenola Manic 2 Gwendaline Lledo Delphine Lissa 3, 4, 5, 6 Christelle Reynes 7 Nathalie Morin Frédéric Chibon 8, 9 Antonella Sistigu Maria Castedo Ilio Vitale 2, 10, * Guido Kroemer 11, 12, 13, Ariane Abrieu 1, CRBM, CNRS UMR5237, Université de Montpellier, France Regina Elena National Cancer Institute, Rome, Italy 3 Paris-Sud/Paris XI, Le Kremlin-Bicêtre, 4 INSERM, UMRS1138, Paris, 5 Equipe 11 Labelisée par la Ligue Nationale Contre le Cancer, Centre Recherche des...
The natural anthraquinone derivative emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a component of several Chinese medicinal herbal preparations utilized for more than 2000 years. substance has been used against diverse disorders including malignancy, inflammation and microbial infection. effective in part by triggering suicidal death or apoptosis. Similar to apoptosis nucleated cells erythrocytes may enter erythrocyte eryptosis, characterized cell shrinkage membrane scrambling with...
The CDC25B inhibitor NSC-95397 triggers apoptosis of tumor cells and is thus considered for the treatment malignancy. substance effective in part by modification gene expression. Similar to nucleated erythrocytes may undergo eryptosis, suicidal erythrocyte death characterized cell shrinkage membrane scrambling with phosphatidylserine translocation surface. Eryptosis be triggered increase cytosolic Ca2+ activity ([Ca2+]i), oxidative stress, ceramide, as well activation protein kinases....
Tetraploid cells may constitute a metastable intermediate between normal euploidy and cancer-associated aneuploidy. are relatively more resistant against DNA damaging agents genetically unstable, due to their tendency towards multipolar, asymmetric division. Therefore, it is important develop strategies for the selective removal of tetraploid cells. Here, we show that targeting mitotic kinesin Eg5 (also known as spindle protein, KSP) by small interfering RNA (siRNA) or pharmacological...
Mitotic catastrophe can be defined as a cell death mode that occurs during or shortly after prolonged/aberrant mitosis, and show apoptotic necrotic features. However, conventional procedures for the detection of apoptosis necrosis, including biochemical bulk assays cytofluorometric techniques, cannot discriminate among pre-mitotic, mitotic post-mitotic death, hence are inappropriate to monitor catastrophe. To address this issue, we generated isogenic human colon carcinoma lines differ in...
Reversine is a small synthetic molecule that inhibits multiple mitotic kinases, including MPS1 as well Aurora kinase A and B (AURKA AURKB). Here, we investigated the effects of reversine on p53-deficient vs p53-proficient cancer cells. We found low doses (~0.5 µM) reversine, which selectively inhibit hence impair spindle assembly checkpoint, kill human TP53 (-/-) colon carcinoma cells less efficiently than their wild-type counterparts. In sharp contrast, high (~5 induced hyperploidization...
Background/Aims: The phytochemical polyphenol rottlerin is a potent activator of diverse Ca2+ -sensitive K+ channels. Those channels play decisive role in the execution eryptosis, suicidal death erythrocytes, which characterized by cell shrinkage and membrane scrambling with phosphatidylserine translocation to erythrocyte surface. Signaling involved stimulation eryptosis includes increase cytosolic activity ([Ca2+]i) ceramide. present study explored, whether induces and, if so, test for...
Abstract Neuroblastoma is the most common paediatric cancer type. Patients diagnosed with high-risk neuroblastoma have poor prognosis and occasionally tumours relapse. As a result, novel treatment strategies are needed for relapse refractory patients. Here, we found that high expression of Mps1 kinase (mitotic Monopolar Spindle 1) was associated relapse-free patient outcomes overall survival. Silencing inhibition in or PDX-derived cells promoted cell apoptosis via caspase-dependent...
Abstract Chorea-Acanthocytosis (ChAc), a neurodegenerative disorder, results from loss-of-function-mutations of chorein-encoding gene VPS13A. In tumour cells chorein up-regulates ORAI1, Ca 2+ -channel accomplishing store operated -entry (SOCE) upon stimulation by STIM1. Furthermore SOCE could be up-regulated lithium. The present study explored whether impacts on neuron apoptosis. Cortical neurons were differentiated induced pluripotent stem generated fibroblasts ChAc patients and healthy...
Background: The widely expressed protein chorein fosters activation of the phosphoinositide 3 kinase (PI3K) pathway thus supporting cell survival. Loss function mutations encoding gene VPS13A (vacuolar sorting-associated 13A) causes chorea-acanthocytosis (ChAc), a neurodegenerative disorder paralleled by deformations erythrocytes. In mice, genetic knockout leads to enhanced neuronal apoptosis. PI3K dependent signalling upregulates Orai1, pore forming channel accomplishing store operated Ca2+...
Background/Aims: The bis-indole alkaloid Fascaplysin is effective against malignancy, an effect at least partially due to stimulation of tumor cell apoptosis. Similar apoptosis nucleated cells, erythrocytes could enter suicidal erythrocyte death or eryptosis, characterized by shrinkage and membrane scrambling with phosphatidylserine translocation the surface. Triggers eryptosis include increase cytosolic Ca2+ activity ([Ca2+]i), oxidative stress ceramide. present study explored, whether...